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. 1992 Dec;77(4):597–603.

Diversity of anti-HLA-DR antibodies elicited by distinct anti-idiotypic monoclonal antibodies recognizing idiotopes co-expressed by the immunizing monoclonal antibody.

S M Mariani 1, E A Armandola 1, S Ferrone 1
PMCID: PMC1421656  PMID: 1283602

Abstract

Analysis at the clonal level of the idiotypic network has identified differences in fine specificity between antigen-binding anti-anti-idiotypic (anti-anti-id) monoclonal antibody (mAb) and the original mAb as well as among antigen-binding anti-anti-idiotypic (anti-id) mAb. However, the diversity of humoral immune responses elicited by anti-id mAb recognizing idiotopes co-expressed on the immunizing mAb has not been analysed. Since this information may contribute to our understanding of the role of anti-id antibodies in the generation of diversity in the course of an immune response, we have compared the fine specificity and idiotype profile of two subsets of anti-HLA-DR mAb generated with the anti-id mAb F5-444 and F5-830. The latter mAb recognize idiotopes co-expressed in the antigen-combining site of the immunizing anti-HLA-DR1,4,w14,w8,9 mAb AC1.59. These investigations showed that: (1) the two subsets of anti-HLA-DR mAb overlap only partially in their reactivity patterns with HLA-DR+ cells; (2) both subsets of anti-HLA-DR mAb recognize spatially close epitopes; (3) each subset of anti-HLA-DR mAb has unique reactivity patterns with soluble HLA-DRw16 and DRw17 antigens; and (4) each subset of anti-anti-id mAb displays a distinct idiotype profile. The subtle differences in the fine specificity and idiotype profile of the two subsets of anti-HLA-DR mAb suggest that anti-id antibodies may play a role in the generation of diversity in the course of a humoral immune response.

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Selected References

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  1. Carpenter A. B., Kahl L. E., Bartkowiak C. D., Duquesnoy R. J., Eisenbeis C. H., Jr Adult rheumatoid arthritis is associated with MC1, a new HLA-D encoded determinant. J Rheumatol. 1988 Mar;15(3):395–399. [PubMed] [Google Scholar]
  2. Crepaldi T., Richiardi P., Malavasi F., Carbonara A. O., Igarashi M., Ferrone S. The monoclonal antibody AC1.59 defines a new polymorphic determinant on HLA-DR molecules. Tissue Antigens. 1985 Jul;26(1):25–34. doi: 10.1111/j.1399-0039.1985.tb00931.x. [DOI] [PubMed] [Google Scholar]
  3. Curtoni E. S., Scudeller G., Mattiuz P. L., Savi M., Ceppellini R. Anti-HL-A antibody evolution in recipients of planned transfusions. Tissue Antigens. 1972;2(6):415–426. doi: 10.1111/j.1399-0039.1972.tb00062.x. [DOI] [PubMed] [Google Scholar]
  4. Ferrara G. B., Tosi R. M., Azzolina G., Carminati G., Kissmeyer-Nielsen F. The production of anti HL-A cytotoxic antisera through planned immunization by intravenous injection of small aliquots of whole blood. I. Immunization of unrelated recipients. Tissue Antigens. 1972;2(5):359–373. doi: 10.1111/j.1399-0039.1972.tb00055.x. [DOI] [PubMed] [Google Scholar]
  5. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Legrand L., Dausset J., Rapaport F. T. Serological and genetic studies of the HL-A system. Results of host immunization within haplo-identical donor-recipient combinations. Transfusion. 1971 Sep-Oct;11(5):233–250. doi: 10.1111/j.1537-2995.1971.tb04409.x. [DOI] [PubMed] [Google Scholar]
  9. Lepage V., Alcalay D., Douay C., Mallet C., Loiseau P., Degos L., Colombani M., Colombani J. Evidence for a new HLA class II determinant present on cells from HLA-DR1 and/or -DR4 individuals. Tissue Antigens. 1985 Feb;25(2):96–102. doi: 10.1111/j.1399-0039.1985.tb00421.x. [DOI] [PubMed] [Google Scholar]
  10. Mariani S. M., Armandola E. A., Ferrone S. Diversity in the fine specificity and idiotypic profile of mouse anti-HLA-DR monoclonal antibody elicited with the syngeneic anti-idiotypic monoclonal antibody F5-830. J Immunol. 1991 Aug 15;147(4):1322–1330. [PubMed] [Google Scholar]
  11. Percy A. J., Harn D. A. Monoclonal anti-idiotypic and anti-anti-idiotypic antibodies from mice immunized with a protective monoclonal antibody against Schistosoma mansoni. J Immunol. 1988 Apr 15;140(8):2760–2762. [PubMed] [Google Scholar]
  12. Perosa F., Ferrone S. Murine antiidiotypic monoclonal antibodies that bear the internal image of HLA-DR allospecificities. J Clin Invest. 1989 Sep;84(3):907–914. doi: 10.1172/JCI114252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Perosa F., Ferrone S. Syngeneic antiidiotypic monoclonal antibodies to the murine anti-HLA-DR,DP monoclonal antibody CR11-462. Hum Immunol. 1988 Dec;23(4):255–269. doi: 10.1016/0198-8859(88)90061-4. [DOI] [PubMed] [Google Scholar]
  14. Perosa F., Kageshita T., Ono R., Ferrone S. Serological methods to detect anti-idiotypic antibodies. Methods Enzymol. 1989;178:74–90. doi: 10.1016/0076-6879(89)78007-1. [DOI] [PubMed] [Google Scholar]
  15. Shearer M. H., Lanford R. L., Kennedy R. C. Monoclonal anti-idiotypic antibodies induce humoral immune responses specific for simian virus 40 large tumor antigen in mice. J Immunol. 1990 Aug 1;145(3):932–939. [PubMed] [Google Scholar]
  16. Shinji T., Ono R., Ferrone S. Fine specificity and idiotype diversity of a murine anti-HLA-DQw3 monoclonal antibody elicited with a syngeneic antiidiotypic monoclonal antibody. J Immunol. 1990 Jun 1;144(11):4291–4297. [PubMed] [Google Scholar]
  17. Temponi M., Kageshita T., Perosa F., Ono R., Okada H., Ferrone S. Purification of murine IgG monoclonal antibodies by precipitation with caprylic acid: comparison with other methods of purification. Hybridoma. 1989 Feb;8(1):85–95. doi: 10.1089/hyb.1989.8.85. [DOI] [PubMed] [Google Scholar]
  18. Turco M. C., De Felice M., Corbo L., Morrone G., Mertelsmann R., Ferrone S., Venuta S. Regulatory role of a monomorphic determinant of HLA Class I antigens in T cell proliferation. J Immunol. 1985 Oct;135(4):2268–2273. [PubMed] [Google Scholar]
  19. Viale G., Flamini G., Grassi F., Buffa R., Natali P. G., Pelagi M., Leoni F., Ménard S., Siccardi A. G. Idiotypic replica of an anti-human tumor-associated antigen monoclonal antibody. Analysis of monoclonal Ab1 and Ab3 fine specificity. J Immunol. 1989 Dec 15;143(12):4338–4344. [PubMed] [Google Scholar]

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