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. 1993 Jul;79(3):412–417.

Generation of CD8+ suppressor T cells by protoscoleces of Echinococcus multilocularis in vitro.

T Kizaki 1, M Ishige 1, W Bingyan 1, N K Day 1, R A Good 1, K Onoé 1
PMCID: PMC1421979  PMID: 8104883

Abstract

Addition of protoscoleces (PSC) of Echinococcus multiocularis suppressed proliferative responses in spleen cells stimulated with concanavalin A (Con A) on day 3 of culture. The suppression was not observed in the spleen cell population depleted of CD8+ cells but observed in the population depleted of CD4+ cells, suggesting the involvement of the CD8+ T cells in the apparent suppression. Indeed, flow cytometry analysis revealed that the proportion of CD8+ cells markedly increased in the Con A cultures containing PSC. Furthermore, when spleen cells were co-cultured with PSC alone, marked increase in the proportion of CD8+ cells as well as B220+ cells was observed. Addition of these increasing CD8+ cells suppressed the proliferative responses of fresh spleen cells stimulated with Con A. These findings suggest that the low responsiveness of spleen cells to Con A on day 3 of culture in the presence of PSC is attributable to an active suppressor mechanism including CD8+ T-suppressor cells generated by stimulation with PSC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali-Khan Z. Cellular changes in the lymphoreticular tissues of C57L/J mice infected with Echinococcus multilocularis cysts. Immunology. 1978 May;34(5):831–839. [PMC free article] [PubMed] [Google Scholar]
  2. Ali-Khan Z. Echinococcus multilocularis: cell-mediated immune response in early and chronic alveolar murine hydatidosis. Exp Parasitol. 1978 Dec;46(2):157–165. doi: 10.1016/0014-4894(78)90128-5. [DOI] [PubMed] [Google Scholar]
  3. Baron R. W., Tanner C. E. The effect of immunosuppression on secondary Echinococcus multilocularis infections in mice. Int J Parasitol. 1976 Feb;6(1):37–42. doi: 10.1016/0020-7519(76)90008-4. [DOI] [PubMed] [Google Scholar]
  4. Bloom B. R. Games parasites play: how parasites evade immune surveillance. Nature. 1979 May 3;279(5708):21–26. doi: 10.1038/279021a0. [DOI] [PubMed] [Google Scholar]
  5. Clegg J. A., Smithers S. R. Death of schistosome cercariae during penetration of the skin. II. Penetration of mammalian skin by Schistosoma mansoni. Parasitology. 1968 Feb;58(1):111–128. doi: 10.1017/s0031182000073479. [DOI] [PubMed] [Google Scholar]
  6. Colley D. G. Immune responses to a soluble schistosomal egg antigen preparation during chronic primary infection with Schistosoma mansoni. J Immunol. 1975 Jul;115(1):150–156. [PubMed] [Google Scholar]
  7. Cox D. A., Dixon J. B., Marshall-Clarke S. Transformation induced by Echinococcus granulosus protoscoleces in unprimed murine spleen cells: identity and MHC restriction of participating cell types. Immunology. 1986 Mar;57(3):461–466. [PMC free article] [PubMed] [Google Scholar]
  8. Davies A. J., Hall J. G., Targett G. A., Murray M. The biological significance of the immune response with special reference to parasites and cancer. J Parasitol. 1980 Oct;66(5):705–721. [PubMed] [Google Scholar]
  9. Eardley D. D., Jayawardena A. N. Suppressor cells in mice infected with Trypanosoma brucei. J Immunol. 1977 Sep;119(3):1029–1033. [PubMed] [Google Scholar]
  10. Kizaki T., Ishige M., Bingyan W., Kumagai M., Day N. K., Good R. A., Onoé K. Interleukin-1-dependent mitogenic responses induced by protoscoleces of Echinococcus multilocularis in murine lymphocytes. J Leukoc Biol. 1993 Mar;53(3):233–239. doi: 10.1002/jlb.53.3.233. [DOI] [PubMed] [Google Scholar]
  11. Kizaki T., Ishige M., Kobayashi S., Bingyan W., Kumagai M., Day N. K., Good R. A., Onoé K. Suppression of T-cell proliferation by CD8+ T cells induced in the presence of protoscolices of Echinococcus multilocularis in vitro. Infect Immun. 1993 Feb;61(2):525–533. doi: 10.1128/iai.61.2.525-533.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kizaki T., Kobayashi S., Ogasawara K., Day N. K., Good R. A., Onoé K. Immune suppression induced by protoscoleces of Echinococcus multilocularis in mice. Evidence for the presence of CD8dull suppressor cells in spleens of mice intraperitoneally infected with E. multilocularis. J Immunol. 1991 Sep 1;147(5):1659–1666. [PubMed] [Google Scholar]
  13. Kroeze W. K., Tanner C. E. Echinococcus multilocularis: susceptibility and responses to infection in inbred mice. Int J Parasitol. 1987 Apr;17(4):873–883. doi: 10.1016/0020-7519(87)90003-8. [DOI] [PubMed] [Google Scholar]
  14. Onoé K., Iwabuchi K., Katsume C., Gotohda T., Arase A., Hatakeyama S., Mishima M., Good R. A., Ogasawara K. A study on graft-versus-host reaction (GVHR) by Simonsen's splenomegaly assay. Cells and antigen systems involved in induction of GVHR. Acta Pathol Jpn. 1989 Feb;39(2):101–110. doi: 10.1111/j.1440-1827.1989.tb01487.x. [DOI] [PubMed] [Google Scholar]
  15. Reinherz E. L., Penta A. C., Hussey R. E., Schlossman S. F. A rapid method for separating functionally intact human T lymphocytes with monoclonal antibodies. Clin Immunol Immunopathol. 1981 Nov;21(2):257–266. doi: 10.1016/0090-1229(81)90214-2. [DOI] [PubMed] [Google Scholar]
  16. Rickard M. D., Williams J. F. Hydatidosis/cysticercosis: immune mechanisms and immunization against infection. Adv Parasitol. 1982;21:229–296. doi: 10.1016/s0065-308x(08)60277-8. [DOI] [PubMed] [Google Scholar]
  17. Riley E. M., Dixon J. B., Jenkins P., Ross G. Echinococcus granulosus infection in mice: host responses during primary and secondary infection. Parasitology. 1986 Apr;92(Pt 2):391–403. doi: 10.1017/s0031182000064155. [DOI] [PubMed] [Google Scholar]
  18. Wilson J. F., Rausch R. L. Alveolar hydatid disease. A review of clinical features of 33 indigenous cases of Echinococcus multilocularis infection in Alaskan Eskimos. Am J Trop Med Hyg. 1980 Nov;29(6):1340–1355. [PubMed] [Google Scholar]

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