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. 1993 May;79(1):146–151.

Interleukin-9 potentiates the interleukin-4-induced IgE and IgG1 release from murine B lymphocytes.

C Petit-Frere 1, B Dugas 1, P Braquet 1, J M Mencia-Huerta 1
PMCID: PMC1422055  PMID: 8509135

Abstract

Interleukin-9 (IL-9) is a mouse T-cell-derived cytokine that supports the growth of mucosal type mast cells suggesting its role in the regulation of type I hypersensitivity reactions. Therefore the possible effect of IL-9 on the in vitro regulation of IL-4-induced IgE and IgG1 releases in the mouse was investigated. In this report, we present evidence that IL-9 potentiated IL-4-induced IgE and IgG1 releases from lipopolysaccharide (LPS)-primed murine B lymphocytes, whereas IL-9 alone was ineffective. The potentiating effect of IL-9 is specific for IgE and IgG1 since no effect on IgM production was observed. This potentiating effect was neither related to an enhanced cell viability, nor to an alteration of the IL-4-induced expression of class II antigens by murine B cells. Besides the fact that IL-9 increased the number of IgG1-secreting cells, this cytokine might also enhance immunoglobulin release on a cell basis. Taken together, these data suggest that IL-9 plays an in vitro regulatory role in antibody synthesis, probably via a direct action on murine B lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akira S., Hirano T., Taga T., Kishimoto T. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 1990 Aug;4(11):2860–2867. [PubMed] [Google Scholar]
  2. Coffman R. L., Ohara J., Bond M. W., Carty J., Zlotnik A., Paul W. E. B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells. J Immunol. 1986 Jun 15;136(12):4538–4541. [PubMed] [Google Scholar]
  3. Conrad D. H., Keegan A. D., Kalli K. R., Van Dusen R., Rao M., Levine A. D. Superinduction of low affinity IgE receptors on murine B lymphocytes by lipopolysaccharide and IL-4. J Immunol. 1988 Aug 15;141(4):1091–1097. [PubMed] [Google Scholar]
  4. Conrad D. H., Waldschmidt T. J., Lee W. T., Rao M., Keegan A. D., Noelle R. J., Lynch R. G., Kehry M. R. Effect of B cell stimulatory factor-1 (interleukin 4) on Fc epsilon and Fc gamma receptor expression on murine B lymphocytes and B cell lines. J Immunol. 1987 Oct 1;139(7):2290–2296. [PubMed] [Google Scholar]
  5. Druez C., Coulie P., Uyttenhove C., Van Snick J. Functional and biochemical characterization of mouse P40/IL-9 receptors. J Immunol. 1990 Oct 15;145(8):2494–2499. [PubMed] [Google Scholar]
  6. Finkelman F. D., Katona I. M., Urban J. F., Jr, Holmes J., Ohara J., Tung A. S., Sample J. V., Paul W. E. IL-4 is required to generate and sustain in vivo IgE responses. J Immunol. 1988 Oct 1;141(7):2335–2341. [PubMed] [Google Scholar]
  7. Finkelman F. D., Katona I. M., Urban J. F., Jr, Holmes J., Ohara J., Tung A. S., Sample J. V., Paul W. E. IL-4 is required to generate and sustain in vivo IgE responses. J Immunol. 1988 Oct 1;141(7):2335–2341. [PubMed] [Google Scholar]
  8. Finkelman F. D., Snapper C. M., Mountz J. D., Katona I. M. Polyclonal activation of the murine immune system by a goat antibody to mouse IgD. IX. Induction of a polyclonal IgE response. J Immunol. 1987 May 1;138(9):2826–2830. [PubMed] [Google Scholar]
  9. Hültner L., Druez C., Moeller J., Uyttenhove C., Schmitt E., Rüde E., Dörmer P., Van Snick J. Mast cell growth-enhancing activity (MEA) is structurally related and functionally identical to the novel mouse T cell growth factor P40/TCGFIII (interleukin 9). Eur J Immunol. 1990 Jun;20(6):1413–1416. doi: 10.1002/eji.1830200632. [DOI] [PubMed] [Google Scholar]
  10. Hültner L., Moeller J. Mast cell growth-enhancing activity (MEA) stimulates interleukin 6 production in a mouse bone marrow-derived mast cell line and a malignant subline. Exp Hematol. 1990 Sep;18(8):873–877. [PubMed] [Google Scholar]
  11. Layton J. E., Vitetta E. S., Uhr J. W., Krammer P. H. Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s). J Exp Med. 1984 Dec 1;160(6):1850–1863. doi: 10.1084/jem.160.6.1850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lue C., Kiyono H., McGhee J. R., Fujihashi K., Kishimoto T., Hirano T., Mestecky J. Recombinant human interleukin 6 (rhIL-6) promotes the terminal differentiation of in vivo-activated human B cells into antibody-secreting cells. Cell Immunol. 1991 Feb;132(2):423–432. doi: 10.1016/0008-8749(91)90039-e. [DOI] [PubMed] [Google Scholar]
  13. Moeller J., Hültner L., Schmitt E., Breuer M., Dörmer P. Purification of MEA, a mast cell growth-enhancing activity, to apparent homogeneity and its partial amino acid sequencing. J Immunol. 1990 Jun 1;144(11):4231–4234. [PubMed] [Google Scholar]
  14. Moon H. B., Severinson E., Heusser C., Johansson S. G., Möller G., Persson U. Regulation of IgG1 and IgE synthesis by interleukin 4 in mouse B cells. Scand J Immunol. 1989 Sep;30(3):355–361. doi: 10.1111/j.1365-3083.1989.tb01221.x. [DOI] [PubMed] [Google Scholar]
  15. Sarfati M., Delespesse G. Possible role of human lymphocyte receptor for IgE (CD23) or its soluble fragments in the in vitro synthesis of human IgE. J Immunol. 1988 Oct 1;141(7):2195–2199. [PubMed] [Google Scholar]
  16. Savelkoul H. F., Termeulen J., Coffman R. L., Van der Linde-Preesman R. A. Frequency analysis of functional Ig C epsilon gene expression in the presence and absence of interleukin 4 in lipopolysaccharide-reactive murine B cells from high and low IgE responder strains. Eur J Immunol. 1988 Aug;18(8):1209–1215. doi: 10.1002/eji.1830180810. [DOI] [PubMed] [Google Scholar]
  17. Sedgwick J. D., Holt P. G. A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J Immunol Methods. 1983 Feb 25;57(1-3):301–309. doi: 10.1016/0022-1759(83)90091-1. [DOI] [PubMed] [Google Scholar]
  18. Snapper C. M., Finkelman F. D., Paul W. E. Regulation of IgG1 and IgE production by interleukin 4. Immunol Rev. 1988 Feb;102:51–75. doi: 10.1111/j.1600-065x.1988.tb00741.x. [DOI] [PubMed] [Google Scholar]
  19. Thiele D. L., Kurosaka M., Lipsky P. E. Phenotype of the accessory cell necessary for mitogen-stimulated T and B cell responses in human peripheral blood: delineation by its sensitivity to the lysosomotropic agent, L-leucine methyl ester. J Immunol. 1983 Nov;131(5):2282–2290. [PubMed] [Google Scholar]
  20. Uyttenhove C., Simpson R. J., Van Snick J. Functional and structural characterization of P40, a mouse glycoprotein with T-cell growth factor activity. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6934–6938. doi: 10.1073/pnas.85.18.6934. [DOI] [PMC free article] [PubMed] [Google Scholar]

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