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. 1993 Nov;80(3):343–351.

Distribution of Fc gamma receptors on trophoblast during human placental development: an immunohistochemical and immunoblotting study.

S D Wainwright 1, C H Holmes 1
PMCID: PMC1422222  PMID: 8288310

Abstract

Expression of Fc gamma receptors on human placental trophoblast was investigated by immunostaining and immunoblotting using a panel of Fc gamma receptor monoclonal antibodies (mAb). Fc gamma receptors typical of other cell types were not detected on syncytiotrophoblast in term placentae when transplacental IgG transport was maximal. Unexpectedly, however, and by contrast with term, all Fc gamma receptor III mAb tested bound to first trimester placental syncytiotrophoblast by immunostaining. Reactivity was relatively restricted and varied between specimens. Fc gamma receptor III products of 41,000-45,000 and 49,000-52,000 MW were consistently detected on first trimester trophoblast membranes by immunoblotting and levels of these products were greatly reduced following treatment with phosphatidylinositol-specific phospholipase C, suggesting that the early trophoblast Fc gamma receptor III is glycosyl-phosphatidylinositol (GPI) linked. The mAb Leu-11b behaved differently to other anti-Fc gamma receptor III mAb examined. By immunostaining, Leu-11b bound to syncytiotrophoblast at term and detected both syncytiotrophoblast and underlying cytotrophoblast in the first trimester. In addition to the GPI-anchored Fc gamma receptor III in first trimester, Leu-11b also detected a 74,000 MW component on both first trimester and term trophoblast membranes by immunoblotting. Thus trophoblast appears to express a GPI-anchored Fc gamma receptor III in first trimester but not term placentae. With the exception of the 74,000 MW Leu-11b-defined product whose function is unclear, currently available Fc gamma receptor mAb appear to be incapable of detecting the protein involved in IgG transport during the later stages of gestation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. L. Isolation of the receptor for IgG from a human monocyte cell line (U937) and from human peripheral blood monocytes. J Exp Med. 1982 Dec 1;156(6):1794–1806. doi: 10.1084/jem.156.6.1794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Down J. A., Kawakami M., Klein M. H., Dorrington K. J. Proteins associated with activity of Fc receptors on isolated human placental syncytiotrophoblast microvillous plasma membranes. Placenta. 1989 May-Jun;10(3):227–246. doi: 10.1016/0143-4004(89)90025-8. [DOI] [PubMed] [Google Scholar]
  3. Fletcher A., Bryant J. A., Gardner B., Judson P. A., Spring F. A., Parsons S. F., Mallinson G., Anstee D. J. New monoclonal antibodies in CD59: use for the analysis of peripheral blood cells from paroxysmal nocturnal haemoglobinuria (PNH) patients and for the quantitation of CD59 on normal and decay accelerating factor (DAF)-deficient erythrocytes. Immunology. 1992 Mar;75(3):507–512. [PMC free article] [PubMed] [Google Scholar]
  4. Fukuda M., Fukuda M. N., Hakomori S. Developmental change and genetic defect in the carbohydrate structure of band 3 glycoprotein of human erythrocyte membrane. J Biol Chem. 1979 May 25;254(10):3700–3703. [PubMed] [Google Scholar]
  5. Holmes C. H., Simpson K. L., Wainwright S. D., Tate C. G., Houlihan J. M., Sawyer I. H., Rogers I. P., Spring F. A., Anstee D. J., Tanner M. J. Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy. J Immunol. 1990 Apr 15;144(8):3099–3105. [PubMed] [Google Scholar]
  6. Huizinga T. W., Kleijer M., Tetteroo P. A., Roos D., von dem Borne A. E. Biallelic neutrophil Na-antigen system is associated with a polymorphism on the phospho-inositol-linked Fc gamma receptor III (CD16). Blood. 1990 Jan 1;75(1):213–217. [PubMed] [Google Scholar]
  7. Huizinga T. W., Roos D., von dem Borne A. E. Fc-gamma receptors: mediators, targets and markers of disease. Baillieres Clin Haematol. 1991 Dec;4(4):889–902. doi: 10.1016/s0950-3536(06)80035-1. [DOI] [PubMed] [Google Scholar]
  8. Huizinga T. W., van der Schoot C. E., Jost C., Klaassen R., Kleijer M., von dem Borne A. E., Roos D., Tetteroo P. A. The PI-linked receptor FcRIII is released on stimulation of neutrophils. Nature. 1988 Jun 16;333(6174):667–669. doi: 10.1038/333667a0. [DOI] [PubMed] [Google Scholar]
  9. Johnson P. M., Brown P. J. Review article: Fc gamma receptors in the human placenta. Placenta. 1981 Oct-Dec;2(4):355–370. doi: 10.1016/s0143-4004(81)80031-8. [DOI] [PubMed] [Google Scholar]
  10. Kamada M., Liang Z., Koide S. S. Identification of IgG and Fc-binding proteins in human seminal plasma and sperm. Arch Androl. 1991 Jul-Aug;27(1):1–7. doi: 10.3109/01485019108987645. [DOI] [PubMed] [Google Scholar]
  11. Kristoffersen E. K., Ulvestad E., Vedeler C. A., Matre R. Fc gamma receptor heterogeneity in the human placenta. Scand J Immunol. 1990 Nov;32(5):561–564. doi: 10.1111/j.1365-3083.1990.tb03197.x. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lanier L. L., Ruitenberg J. J., Phillips J. H. Functional and biochemical analysis of CD16 antigen on natural killer cells and granulocytes. J Immunol. 1988 Nov 15;141(10):3478–3485. [PubMed] [Google Scholar]
  14. Leach L., Eaton B. M., Firth J. A., Contractor S. F. Immunocytochemical and labelled tracer approaches to uptake and intracellular routing of immunoglobulin-G (IgG) in the human placenta. Histochem J. 1991 Oct;23(10):444–449. doi: 10.1007/BF01041374. [DOI] [PubMed] [Google Scholar]
  15. Liang Z. G., Kamada M., Koide S. S. Properties of an immunoglobulin binding factor from human seminal plasma. Biochem Int. 1991 Aug;24(6):1003–1013. [PubMed] [Google Scholar]
  16. López de Castro J. A. Purification of human HLA-A and HLA-B class I histocompatibility antigens. Methods Enzymol. 1984;108:582–600. doi: 10.1016/s0076-6879(84)08119-2. [DOI] [PubMed] [Google Scholar]
  17. Makiya R., Thornell L. E., Stigbrand T. Placental alkaline phosphatase, a GPI-anchored protein, is clustered in clathrin-coated vesicles. Biochem Biophys Res Commun. 1992 Mar 16;183(2):803–808. doi: 10.1016/0006-291x(92)90554-x. [DOI] [PubMed] [Google Scholar]
  18. Matre R., Kleppe G., Tönder O. Isolation and characterization of Fc gamma receptors from human placenta. Acta Pathol Microbiol Scand C. 1981 Jun;89(3):209–213. doi: 10.1111/j.1699-0463.1981.tb02688.x. [DOI] [PubMed] [Google Scholar]
  19. Mikulska J., Boratyński J., Niezgódka M., Lisowski J. Human placental membrane receptor for IgG. Purification of the receptor and its subunit structure. Immunol Lett. 1982 Sep;5(3):137–143. doi: 10.1016/0165-2478(82)90098-0. [DOI] [PubMed] [Google Scholar]
  20. Pearse B. M. Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G. Proc Natl Acad Sci U S A. 1982 Jan;79(2):451–455. doi: 10.1073/pnas.79.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pesando J. M., Stucki M., Hoffman P. Altered expression of surface antigens with appearance of HLA class II molecules on a malignant human B-cell line. Hum Immunol. 1987 Aug;19(4):235–243. doi: 10.1016/0198-8859(87)90041-3. [DOI] [PubMed] [Google Scholar]
  22. Ravetch J. V., Kinet J. P. Fc receptors. Annu Rev Immunol. 1991;9:457–492. doi: 10.1146/annurev.iy.09.040191.002325. [DOI] [PubMed] [Google Scholar]
  23. Sedmak D. D., Davis D. H., Singh U., van de Winkel J. G., Anderson C. L. Expression of IgG Fc receptor antigens in placenta and on endothelial cells in humans. An immunohistochemical study. Am J Pathol. 1991 Jan;138(1):175–181. [PMC free article] [PubMed] [Google Scholar]
  24. Selvaraj P., Rosse W. F., Silber R., Springer T. A. The major Fc receptor in blood has a phosphatidylinositol anchor and is deficient in paroxysmal nocturnal haemoglobinuria. Nature. 1988 Jun 9;333(6173):565–567. doi: 10.1038/333565a0. [DOI] [PubMed] [Google Scholar]
  25. Smith N. C., Brush M. G., Luckett S. Preparation of human placental villous surface membrane. Nature. 1974 Nov 22;252(5481):302–303. doi: 10.1038/252302b0. [DOI] [PubMed] [Google Scholar]
  26. Stuart S. G., Simister N. E., Clarkson S. B., Kacinski B. M., Shapiro M., Mellman I. Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium. EMBO J. 1989 Dec 1;8(12):3657–3666. doi: 10.1002/j.1460-2075.1989.tb08540.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sunderland C. A., Davies J. O., Stirrat G. M. Immunohistology of normal and ovarian cancer tissue with a monoclonal antibody to placental alkaline phosphatase. Cancer Res. 1984 Oct;44(10):4496–4502. [PubMed] [Google Scholar]
  28. Tavaré J. M., Holmes C. H. Differential expression of the receptors for epidermal growth factor and insulin in the developing human placenta. Cell Signal. 1989;1(1):55–64. doi: 10.1016/0898-6568(89)90020-x. [DOI] [PubMed] [Google Scholar]

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