Abstract
An antigen that can be derived from rabbit kidney can be identified by use of suitable rabbit antisera as being non-tissue specific in addition to being an autoantigen, since it had been found to be distributed in kidney, heart, adrenal and liver. The purification of this autoantigen has been accomplished by successive use of four different methods. These were the salting out with ammonium sulphate, gel filtration on Sephadex G-200, dialysis against distilled water, and chromatography on DEAE-cellulose. The heterogeneity of the products derived from the procedure at each step was analysed by means of analytical electrophoresis and ultracentrifugation, and also by gel diffusion precipitation.
The final product was a homogeneous component, according to the criteria that were used. It had a sedimentation coefficient of 4.1S, suggesting a mol. wt of approximately 60,000. This autoantigen is of similar molecular size to several others that are, in contrast, adrenal specific or kidney specific.
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- BAXTER J. H., GOODMAN H. C. Nephrotoxic serum nephritis in rats. I. Distribution and specificity of the antigen responsible for the production of nephrotoxic antibodies. J Exp Med. 1956 Oct 1;104(4):467–485. doi: 10.1084/jem.104.4.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burgio G. R., Severi F., Vaccaro R., Rossoni R. Antibodies reacting with heart tissue in the course of rheumatic fever in children. Schweiz Med Wochenschr. 1966 Apr 2;96(13):431–435. [PubMed] [Google Scholar]
- CENTENO E., SHULMAN S., MILGROM F., WITEBSKY E. IMMUNOLOGICAL STUDIES ON ADRENAL GLANDS. VI. THE MULTIPLE NATURE OF THE RABBIT ADRENAL AUTOANTIGENS. Immunology. 1965 Feb;8:160–169. [PMC free article] [PubMed] [Google Scholar]
- Centeno E. R., Shulman S. Isolation and characterization of a rabbit adrenal autoantigen. Immunology. 1973 May;24(5):901–910. [PMC free article] [PubMed] [Google Scholar]
- Centeno E. R., Shulman S. Physicochemical characterization and isolation of rabbit kidney-specific autoantigens. Immunology. 1973 May;24(5):911–924. [PMC free article] [PubMed] [Google Scholar]
- Centeno E., Shullman S., Milgrom F., Witebsky E., Skelton F. Studies on organ specificity. XVII. Autoantigens of rabbit kidney. J Immunol. 1966 Mar;96(3):530–536. [PubMed] [Google Scholar]
- HILL A. G. S., CRUICKSHANK B., CROSSLAND A. A study of antigenic components of kidney tissue. Br J Exp Pathol. 1953 Feb;34(1):27–34. [PMC free article] [PubMed] [Google Scholar]
- Halbert S. P., Holm S. E., Thompson A. Cardiac autoantibodies. I. Immunodiffusion analysis of multiple responses evoked homologously and heterologously. J Exp Med. 1968 Mar 1;127(3):613–631. doi: 10.1084/jem.127.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KRAKOWER C. A., GREENSPON S. A. The localization of the nephrotoxic antigen(s) in extraglomerular tissues; observations including a measure of its concentration in certain locales. AMA Arch Pathol. 1958 Oct;66(4):364–383. [PubMed] [Google Scholar]
- Katz D. H., Unanue E. R., Dixon F. J. Nephritogenic properties of cross-reacting kidney-fixing antibodies to heart, spleen and muscle. J Immunol. 1967 Feb;98(2):260–268. [PubMed] [Google Scholar]
- Kushner I., Kaplan M. H. Antigenic analysis of extracts of human heart tissue: cardiac antigens with limited distribution in other organs. J Immunol. 1967 Sep;99(3):526–533. [PubMed] [Google Scholar]
- MILGROM F., CENTENO E., SHULMAN S., WITEBSKY E. AUTOANTIBODIES RESULTING FROM IMMUNIZATION WITH KIDNEY. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1009–1013. doi: 10.3181/00379727-116-29434. [DOI] [PubMed] [Google Scholar]
- Nagasawa T., Shibata S. Production of glomerulonephritis by antisera against the soluble antigen (tryptic digested ultrasupernatant substance) from various rat organs. Jpn J Exp Med. 1969 Apr;39(2):143–151. [PubMed] [Google Scholar]