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. 1973 May;24(5):901–910.

Isolation and characterization of a rabbit adrenal autoantigen

E R Centeno, S Shulman
PMCID: PMC1422812  PMID: 4197597

Abstract

The antigenic components of adrenal extract were fractionated by chromatography on DEAE-cellulose. Using a stepwise elution procedure, it was found that a componenet of major interest, termed Pk3b, was eluted by a buffer of pH 6.70 and 0.09 M in phosphate-chloride. This antigenic preparation reacted with several rabbit antisera that had been prepared by isoimmunization with rabbit adrenal homogenate, in contrast to several other fractions, which reacted with only one (or two) of the antisera. Pk3b also showed a high degree of homogeneity, as shown by immunodiffusion, paper electrophoresis, and analytical ultracentrifugation. By the latter technique, an extrapolated sedimentation coefficient of 4.45S was determined. The further comparison of this antigen in immunodiffusion, along with an antiserum and the adrenal extract from the antiserum-providing animal, confirmed again that this is an autoantigen, and that it is one of five autoantigens of the adrenal gland.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CENTENO E., SHULMAN S., MILGROM F., WITEBSKY E. IMMUNOLOGICAL STUDIES ON ADRENAL GLANDS. VI. THE MULTIPLE NATURE OF THE RABBIT ADRENAL AUTOANTIGENS. Immunology. 1965 Feb;8:160–169. [PMC free article] [PubMed] [Google Scholar]
  2. Chao L. P., Einstein E. R. Isolation and characterization of an active fragment from enzymatic degradation of encephalitogenic protein. J Biol Chem. 1968 Nov 25;243(22):6050–6055. [PubMed] [Google Scholar]
  3. Eylar E. H., Hashim G. A. Allergic encephalomyelitis: the structure of encephalitogenic determinant. Proc Natl Acad Sci U S A. 1968 Oct;61(2):644–650. doi: 10.1073/pnas.61.2.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ghayasuddin M., Shulman S. Studies on thyroid proteins. VII. Immunochemical properties of some enzymatic fragments of rabbit thyroglobulin. Immunology. 1970 Aug;19(2):225–237. [PMC free article] [PubMed] [Google Scholar]
  5. KIES M. W., THOMPSON E. B., ALVORD E. C., Jr THE RELATIONSHIP OF MYELIN PROTEINS TO EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS. Ann N Y Acad Sci. 1965 Mar 31;122:148–160. doi: 10.1111/j.1749-6632.1965.tb20199.x. [DOI] [PubMed] [Google Scholar]
  6. Karitzky D., Burtin P. Isolement de l'autoantigène responsable de la formation d'autoanticorps chez les malades atteints de cancer gastrique. Eur J Biochem. 1967 Jun;1(4):411–418. doi: 10.1111/j.1432-1033.1967.tb00089.x. [DOI] [PubMed] [Google Scholar]
  7. Kibler R. F., Shapira R., McKneally S., Jenkins J., Selden P., Chou F. Encephalitogenic protein: structure. Science. 1969 May 2;164(3879):577–580. doi: 10.1126/science.164.3879.577. [DOI] [PubMed] [Google Scholar]
  8. Loisillier F., Burtin P., Grabar P. Isolement et caractérisation de l'auto-antigène responsable de la formation d'auto-anticorps chez les malades atteints de lésions mammaires (cancéruses ou non) Ann Inst Pasteur (Paris) 1968 Nov;115(5):829–840. [PubMed] [Google Scholar]
  9. Roitt I. M., Torrigiani G., Doniach D. Immunochemical studies on the thyroglobulin autoantibody system in human thyroiditis. Immunology. 1968 Nov;15(5):681–696. [PMC free article] [PubMed] [Google Scholar]
  10. SALVATORE G., SALVATORE M., CAHNMANN H. J., ROBBINS J. SEPARATION OF THYROIDAL IODOPROTEINS AND PURIFICATION OF THYROGLOBULIN BY GEL FILTRATION AND DENSITY GRADIENT CENTRIFUGATION. J Biol Chem. 1964 Oct;239:3267–3274. [PubMed] [Google Scholar]
  11. SHULMAN S., ARMENIA J. P. STUDIES ON THYROID PROTEINS. I. THE COMPONENTS OF HOG THYROID TISSUE, AND THE PREPARATION OF PURIFIED THYROGLOBULIN, BY COLUMN CHROMATOGRAPHY. J Biol Chem. 1963 Aug;238:2723–2731. [PubMed] [Google Scholar]
  12. Shulman S., Centeno E., Milgrom F., Witebsky E. Immunological studies on adrenal glands. V. Chemical studies on the rabbit adrenal-specific autoantigen. Immunology. 1965 Jun;8(6):531–538. [PMC free article] [PubMed] [Google Scholar]
  13. Shulman S., Mates G., Bronson P. Studies on thyroid proteins. II. Purification of human thyroid proteins by gel filtration and the isolation of 19-S, 7-S, and 4-S components. Biochim Biophys Acta. 1967 Oct 23;147(2):208–221. [PubMed] [Google Scholar]
  14. UI N., TARUTANI O., KONDO Y., TAMURA H. Chromatographic fractionation of hog thyroglobulin. Nature. 1961 Sep 16;191:1199–1201. doi: 10.1038/1911199a0. [DOI] [PubMed] [Google Scholar]
  15. Voisin G. A., Toullet F. Etude sur l'orchite aspermatogénétique autoimmune et les autoantigènes de spermatozoides chez le cobaye. Ann Inst Pasteur (Paris) 1968 Jun;114(6):727–755. [PubMed] [Google Scholar]
  16. WITEBSKY E., MILGROM F. Immunological studies on adrenal glands. II. Immunization with adrenals of the same species. Immunology. 1962 Jan;5:67–78. [PMC free article] [PubMed] [Google Scholar]

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