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. 1973 Jan;24(1):163–176.

Studies of porcine secretory IgA and its component chains in relation to intestinal absorption of colostral immunoglobulins by the neontal pig

P Porter
PMCID: PMC1422872  PMID: 4631061

Abstract

Porcine secretory component was identified after reductive dissociation of secretory IgA and isolated in the free form from sow milk.

Electrophoretically fast polypeptide chains were detected in the light chain fraction of reduced and alkylated porcine secretory IgA. These probably relate to J chain and were immunologically and electrophoretically distinct from secretory component. J chains were not present in the light chain fractions of reduced and alkylated porcine 7S IgG and 6.4S IgA.

Porcine colostral IgA occurs in a wide range of molecular sizes and IgA lacking secretory component having S20, W 6.4 and 9.3 was isolated from colostrum and serum of suckling piglets.

A haemagglutination inhibition assay for 10.6S secretory IgA using specific rabbit anti porcine secretory component demonstrated that only a very small proportion of the IgA derived from colostrum is 10.6S IgA with secretory component.

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Selected References

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  1. AUGUSTIN R., HAYWARD B. J. Human reagins to grass pollens and moulds: their purification and physico-chemical characterization. Immunology. 1960 Jan;3:45–73. [PMC free article] [PubMed] [Google Scholar]
  2. Adinolfi M., Glynn A. A., Lindsay M., Milne C. M. Serological properties of gamma-A antibodies to Escherichia coli present in human colostrum. Immunology. 1966 Jun;10(6):517–526. [PMC free article] [PubMed] [Google Scholar]
  3. BRAMBELL F. W., HEMMINGS W. A., OAKLEY C. L., PORTER R. R. The relative transmission of the fractions of papain hydrolyzed homologous gamma-globulin from the uterine cavity to the foetal circulation in the rabbit. Proc R Soc Lond B Biol Sci. 1960 Mar 1;151:478–482. doi: 10.1098/rspb.1960.0011. [DOI] [PubMed] [Google Scholar]
  4. Bourne F. J. IgA immunoglobulin from porcine serum. Biochem Biophys Res Commun. 1969 Jul 7;36(1):138–145. doi: 10.1016/0006-291x(69)90660-3. [DOI] [PubMed] [Google Scholar]
  5. Brambell F. W. The transmission of immune globulins from the mother to the foetal and newborn young. Proc Nutr Soc. 1969 Mar;28(1):35–41. [PubMed] [Google Scholar]
  6. Brandtzaeg P. Hidden antigenic determinant in secretory immunoglobulin A. Nature. 1968 Oct 19;220(5164):292–293. doi: 10.1038/220292a0. [DOI] [PubMed] [Google Scholar]
  7. Butler W. T., Rossen R. D., Waldmann T. A. The mechanism of appearance of immunoglobulin A in nasal secretions in man. J Clin Invest. 1967 Dec;46(12):1883–1893. doi: 10.1172/JCI105678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. CHODIRKER W. B., TOMASI T. B., Jr GAMMA-GLOBULINS: QUANTITATIVE RELATIONSHIPS IN HUMAN SERUM AND NONVASCULAR FLUIDS. Science. 1963 Nov 22;142(3595):1080–1081. doi: 10.1126/science.142.3595.1080. [DOI] [PubMed] [Google Scholar]
  9. Cebra J. J., Small P. A., Jr Polypeptide chain structure of rabbit immunoglobulins. 3. Secretory gamma-A-immunoglobulin from colostrum. Biochemistry. 1967 Feb;6(2):503–512. doi: 10.1021/bi00854a019. [DOI] [PubMed] [Google Scholar]
  10. Curtis J., Bourne F. J. Immunoglobulin quantitation in sow serum, colostrum and milk and the serum of young pigs. Biochim Biophys Acta. 1971 Apr 27;236(1):319–332. doi: 10.1016/0005-2795(71)90181-4. [DOI] [PubMed] [Google Scholar]
  11. Mach J. P., Pahud J. J., Isliker H. IgA with "secretory piece" in bovine colostrum and saliva. Nature. 1969 Aug 30;223(5209):952–955. doi: 10.1038/223952a0. [DOI] [PubMed] [Google Scholar]
  12. Noll H. Characterization of macromolecules by constant velocity sedimentation. Nature. 1967 Jul 22;215(5099):360–363. doi: 10.1038/215360a0. [DOI] [PubMed] [Google Scholar]
  13. ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]
  14. Pahud J. J., Mach J. P. Identification of secretory IgA, free secretory piece and serum IgA in the ovine and caprine species. Immunochemistry. 1970 Aug;7(8):679–686. doi: 10.1016/0019-2791(70)90174-6. [DOI] [PubMed] [Google Scholar]
  15. Perelmutter L., Khera K. A study on the detection of human reagins with rat peritoneal mast cells. Int Arch Allergy Appl Immunol. 1970;39(1):27–44. doi: 10.1159/000230331. [DOI] [PubMed] [Google Scholar]
  16. Porter P. Immunoglobulin IgA in bovine mammary secretions and serum of the neonatal calf. Biochim Biophys Acta. 1971 Jun 29;236(3):664–674. doi: 10.1016/0005-2795(71)90253-4. [DOI] [PubMed] [Google Scholar]
  17. Porter P., Noakes D. E., Allen W. D. Secretory IgA and antibodies to Escherichia coli in porcine colostrum and milk and their significance in the alimentary tract of the young pig. Immunology. 1970 Feb;18(2):245–257. [PMC free article] [PubMed] [Google Scholar]
  18. Porter P., Noakes D. E. Immunoglobulins in sow mammary secretions throughout lactation and their significance as anti-(Escherichia coli) antibodies to the young pig. Biochem J. 1969 Jun;113(2):6P–7P. doi: 10.1042/bj1130006pb. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Porter P. Porcine colostral IgA and IgM antibodies to Escherichia coli and their intestinal absorption by the neonatal piglet. Immunology. 1969 Oct;17(4):617–626. [PMC free article] [PubMed] [Google Scholar]
  20. Porter P. Transfer of immunoglobulins IgG, IgA and IgM to lacteal secretions in the parturient sow and their absorption by the neonatal piglet. Biochim Biophys Acta. 1969 Jul 1;181(2):381–392. doi: 10.1016/0005-2795(69)90271-2. [DOI] [PubMed] [Google Scholar]
  21. Richardson A. K., Kelleher P. C. The 11-S sow colostral immunoglobulin. Isolation and physicochemical properties. Biochim Biophys Acta. 1970 Jul 27;214(1):117–124. doi: 10.1016/0005-2795(70)90075-9. [DOI] [PubMed] [Google Scholar]
  22. Ricks J., Roberts M., Patterson R. Canine secretory immunoglobulins: identification of secretory component. J Immunol. 1970 Dec;105(6):1327–1333. [PubMed] [Google Scholar]
  23. Rossen R. D., Alford R. H., Butler W. T., Vannier W. E. The separation and characterization of proteins intrinsic to nasal secretion. J Immunol. 1966 Sep;97(3):369–378. [PubMed] [Google Scholar]
  24. TOMASI T. B., Jr, TAN E. M., SOLOMON A., PRENDERGAST R. A. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. doi: 10.1084/jem.121.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]

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