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. 1973 Dec;25(6):967–980.

Glomerular basement membrane antigens of Masugi nephritis

R P Misra
PMCID: PMC1422989  PMID: 4357226

Abstract

Glomerular basement membrane (GBM) isolated from rat, dog and human kidneys were treated with enzymes to obtain soluble membrane preparations. Gel filtration on calibrated columns of soluble membrane preparations produced several macromolecules which were identified as glycoproteins and glycopeptides, depending on their molecular sizes. A sialoglycoprotein of 50,000 Daltons is shown to be the nephrotoxigenic antigen of the GBM. Lipids were shown to be a non-essential component of the nephrotoxigenic antigen. Collagen and several other glycopeptides were found to be non-nephrotoxigenic antigens of the GBM. Use of pronase or combinations of certain enzymes (for example, collagenase and pronase, pepsin and trypsin) to obtain the soluble components of the membrane led to an alteration of the nephrotoxigenic antigen molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CRUICKSHANK B., HILL A. G. The histochemical identification of a connective-tissue antigen in the rat. J Pathol Bacteriol. 1953 Jul;66(1):283–289. doi: 10.1002/path.1700660132. [DOI] [PubMed] [Google Scholar]
  2. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  3. GOODMAN M., GREENSPON S. A., KRAKOWER C. A. The antigenic composition of the various anatomic structures of the canine kidney. J Immunol. 1955 Aug;75(2):96–104. [PubMed] [Google Scholar]
  4. Huang F., Kalant N. Isolation and characterization of antigenic components of rat glomerular basement membrane. Can J Biochem. 1968 Dec;46(12):1523–1532. doi: 10.1139/o68-226. [DOI] [PubMed] [Google Scholar]
  5. JACOBS S. Determination of nitrogen in proteins by means of idanetrione hydrate. Nature. 1959 Jan 24;183(4656):262–262. doi: 10.1038/183262a0. [DOI] [PubMed] [Google Scholar]
  6. KRAKOWER C. A., GREENSPON S. A. Localization of the nephrotoxic antigen within the isolated renal glomerulus. AMA Arch Pathol. 1951 Jun;51(6):629–639. [PubMed] [Google Scholar]
  7. KRAKOWER C. A., GREENSPON S. A. The localization of the nephrotoxic antigen(s) in extraglomerular tissues; observations including a measure of its concentration in certain locales. AMA Arch Pathol. 1958 Oct;66(4):364–383. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Misra R. P., Berman L. B. Glomerular basement membrane: insights from molecular models. Am J Med. 1969 Sep;47(3):337–339. doi: 10.1016/0002-9343(69)90217-4. [DOI] [PubMed] [Google Scholar]
  10. Misra R. P., Berman L. B. Studies on glomerular basement membrane. 1. Isolation and chemical analysis of normal glomerular basement membrane. Proc Soc Exp Biol Med. 1966 Jul;122(3):705–710. doi: 10.3181/00379727-122-31232. [DOI] [PubMed] [Google Scholar]
  11. Misra R. P., Berman L. B. Studies on glomerular basement membrane. 3. Effects of steroid on membrane chemistry and its protein permeability. Lab Invest. 1972 Jun;26(6):666–670. [PubMed] [Google Scholar]
  12. Misra R. P., Berman L. B. Studies on glomerular basement membrane. II. Isolation and chemical analysis of diseased glomerular basement membrane. Lab Invest. 1968 Feb;18(2):131–138. [PubMed] [Google Scholar]
  13. Misra R. P. Isolation of glomeruli from mammalian kidneys by graded sieving. Am J Clin Pathol. 1972 Aug;58(2):135–139. doi: 10.1093/ajcp/58.2.135. [DOI] [PubMed] [Google Scholar]
  14. Misra R. P. Structure of the glomerular basement membrane. Pathobiol Annu. 1971;1:325–351. [PubMed] [Google Scholar]
  15. Nagasawa T., Shibata S. Immunofluorescence studies on the anatomic distribution of the soluble antigen responsible for the production of nephrotoxic serum nephritis. J Immunol. 1969 Oct;103(4):736–740. [PubMed] [Google Scholar]
  16. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. II. Prog Allergy. 1962;6:30–154. doi: 10.1159/000313795. [DOI] [PubMed] [Google Scholar]
  17. PREER J. R., Jr A quantitative study of a technique of double diffusion in agar. J Immunol. 1956 Jul;77(1):52–60. [PubMed] [Google Scholar]
  18. ROTHBARD S., WATSON R. F. Antigenicity of rat collagen. Demonstration of antibody to rat collagen in the renal glomeruli of rats by fluorescence microscopy. J Exp Med. 1961 Jun 1;113:1041–1051. doi: 10.1084/jem.113.6.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rothbard S., Watson R. F. Comparison of reactions of antibodies to rat collagen and to rat kidney in the basement membranes of rat renal glomeruli. J Exp Med. 1969 Jun 1;129(6):1145–1161. doi: 10.1084/jem.129.6.1145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rothbard S., Watson R. F. Demonstration by immunofluorescence of the fixation of perfused antibody to human collagen in human kidney. J Exp Med. 1967 Apr 1;125(4):595–606. doi: 10.1084/jem.125.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. STEBLAY R. W., LEPPER M. H. Some immunologic properties of human and dog glomerular basement membrane. I. Isolation of human glomerular basement membrane; similar or identical complement-fixing antigens in human and dog glomerular basement membrane preparations. J Immunol. 1961 Dec;87:627–635. [PubMed] [Google Scholar]
  22. STEBLAY R. W., LEPPER M. H. Some immunologic properties of human and dog glomerular basement membrane. II. Nephritis produced in dogs by rabbit antihuman glomerular basement membrane sera. J Immunol. 1961 Dec;87:636–646. [PubMed] [Google Scholar]
  23. STEBLAY R. W. Localization in human kidney of antibodies formed in sheep against human placenta. J Immunol. 1962 Apr;88:434–442. [PubMed] [Google Scholar]
  24. Shibata S., Miyakawa Y., Naruse T., Nagasawa T., Takuma T. A glycoprotein that induces nephrotoxic antibody: its isolation and purification from rat glomerular basement membrane. J Immunol. 1969 Mar;102(3):593–601. [PubMed] [Google Scholar]
  25. Shibata S., Naruse T., Nagasawa T., Takuma T., Miyakawa Y. Purification by starch block electrophoresis of renal antigen that induces nephrotoxic antibody. J Immunol. 1967 Aug;99(2):454–464. [PubMed] [Google Scholar]
  26. Skoza L., Mohos S. C. Nephritogenic antigenicity of chromatographic fractions of trypsin and pronase glomerular digests. Proc Soc Exp Biol Med. 1969 Sep;131(4):1189–1193. doi: 10.3181/00379727-131-34067. [DOI] [PubMed] [Google Scholar]
  27. Thoenes G. H., Hammer D. K. Immunchemische Untersuchungen am Nephrotoxischen Antigen der Glomerulären Basalmembran. Clin Chim Acta. 1967 Nov;18(2):253–265. doi: 10.1016/0009-8981(67)90166-0. [DOI] [PubMed] [Google Scholar]
  28. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

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