Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1974 Feb;26(2):233–246.

Antibody production in mice

VI. Effect of anti-carrier antibody on cellular co-operation in the primary anti-hapten antibody response

K Takatsu, T Hamaoka, M Kitagawa
PMCID: PMC1423074  PMID: 4546728

Abstract

We studied the effect of passively administered anti-carrier and anti-hapten antibodies on the primary anti-hapten antibody response to hapten—carrier conjugates in mice. Bacterial α-amylase (BαA), Taka-amylase A (TAA) and keyhole limpet haemocyanin (KLH) were used as carrier molecules, and 2,4-dinitrophenyl (DNP) group was used as a haptenic determinant.

Three groups of mice were injected intravenously with anti-carrier antiserum, anti-hapten antiserum and normal serum as the control, respectively, immediately after the immunization with the hapten—carrier conjugate.

The primary anti-carrier antibody response was markedly suppressed by the passively administered anti-carrier antibody but not anti-hapten antibody. However, the primary anti-hapten antibody response was suppressed not only by passively administered anti-hapten antibody but also by the injection of anti-carrier antibody in an early period after the immunization.

When anti-carrier antibody was given twice 0 and 7 days after immunization, the primary anti-hapten antibody response was markedly suppressed and the suppressive effect was observed even at a later period. In contrast, anti-hapten antibody given by the same schedule as above suppressed only the primary anti-hapten antibody response, but not the anti-carrier antibody response.

Passively administered anti-carrier antibody did not suppress carrier-specific helper cell development, but still suppressed the development of B memory cells and antibody formation against carrier determinants. The antigen dose required for the development of B-cell memory was much higher than that necessary for the stimulation of T cells.

Passively administered anti-carrier antibody clearly inhibited the cellular cooperation between carrier-committed helper cells and hapten-specific B cells and the augmented primary anti-hapten antibody response induced by carrier-primed T cells was clearly abolished. Furthermore, mice preimmunized with carrier showed significantly lower anti-hapten antibody response following the hapten—carrier challenge. Moreover, development of hapten-specific memory cells was also suppressed.

Thus, even in the non-specific antibody-induced suppression by anti-carrier antibody, negative feedback effect of a B-cell product with anti-carrier specificity exclusively regulates the B-cell line development and differentiation.

Full text

PDF
236

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EISEN H. N., KERN M., NEWTON W. T., HELMREICH E. A study of the distribution of 2,4-dinitrobenzene sensitizers between isolated lymph node cells and extracellular medium in relation to induction of contact skin sensitivity. J Exp Med. 1959 Aug 1;110(2):187–206. doi: 10.1084/jem.110.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Feldmann M., Diener E. Antibody-mediated suppression of the immune response in vitro. I. Evidence for a central effect. J Exp Med. 1970 Feb;131(2):247–274. doi: 10.1084/jem.131.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Graf M. W., Uhr J. W. Regulation of antibody formation by serum antibody. I. Removal of specific antibody by means of immunoadsorption. J Exp Med. 1969 Nov 1;130(5):1175–1186. doi: 10.1084/jem.130.5.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hamaoka T., Kitagawa M. Antibody production in mice. II. The mechanism of antigenic stimulation in the secondary immune response. Immunology. 1971 Feb;20(2):191–203. [PMC free article] [PubMed] [Google Scholar]
  5. Hamaoka T., Kitagawa M., Matsuoka Y., Yamamura Y. Antibody production in mice. I. The analysis of immunological memory. Immunology. 1969 Jul;17(1):55–69. [PMC free article] [PubMed] [Google Scholar]
  6. Hamaoka T., Takatsu K., Kitagawa M. Antibody production in mice. V. The suppressive effect of anti-carrier antibodies on cellular cooperation in the induction of secondary anti-hapten antibody responses. Immunology. 1973 Mar;24(3):409–424. [PMC free article] [PubMed] [Google Scholar]
  7. Hamaoka T., Takatsu K., Masaki H., Matsuoka Y., Kitagawa M. Antibody production in mice. 3. The suppressive effect of antibody on the initiation of secondary immune response. Immunology. 1971 Jun;20(6):871–881. [PMC free article] [PubMed] [Google Scholar]
  8. Henney C. S., Ishizaka K. Studies on the immunogenicity of antigen-antibody precipitates. II. The suppressive effect of anti-carrier and anti-hapten antibodies on the immunogenicity of dinitrophenylated human gamma G globulin. J Immunol. 1970 Jun;104(6):1540–1549. [PubMed] [Google Scholar]
  9. Kappler J. W., Hoffmann M., Dutton R. W. Regulation of the immune response. I. Differential effect of passively administered antibody on the thymus-derived and bone marrow-derived lymphocytes. J Exp Med. 1971 Sep 1;134(3 Pt 1):577–587. doi: 10.1084/jem.134.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MOLLER G., WIGZELL H. ANTIBODY SYNTHESIS AT THE CELLULAR LEVEL. ANTIBODY-INDUCED SUPPRESSION OF 19S AND 7S ANTIBODY RESPONSE. J Exp Med. 1965 Jun 1;121:969–989. doi: 10.1084/jem.121.6.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McBride R. A., Schierman L. W. Passive antibody and the immune response. Factors which determine enhancement and suppression. J Exp Med. 1971 Oct 1;134(4):833–845. doi: 10.1084/jem.134.4.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pincus C. S., Lamm M. E., Nussenzweig V. Regulation of the immune response: suppressive and enhancing effects of passively administered antibody. J Exp Med. 1971 May 1;133(5):987–1003. doi: 10.1084/jem.133.5.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. ROWLEY D. A., FITCH F. W. HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT. J Exp Med. 1964 Dec 1;120:987–1005. doi: 10.1084/jem.120.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. UHR J. W., BAUMANN J. B. Antibody formation. I. The suppression of antibody formation by passively administered antibody. J Exp Med. 1961 May 1;113:935–957. doi: 10.1084/jem.113.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES