Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1974 Mar;26(3):465–476.

The induction of delayed hypersensitivity by macrophage-associated antigen

The role of macrophage cytophilic antibody

M Zembala, W Ptak, Maria Hanczakowska
PMCID: PMC1423219  PMID: 4854882

Abstract

The regulation of the immune responses by antibody administered passively on peritoneal exudate cells (PEC) was studied in guinea-pigs. The immunogenicity of sheep red blood cells (SRBC) associated with PEC for the induction of delayed hypersensitivity (DH) to soluble erythrocyte antigen was enhanced when PEC were incubated with anti-SRBC antibody. In contrast, the antibody response to SRBC was depressed. This phenomenon was only observed with specific antibody and was partially blocked when PEC covered with anti-SRBC antibody were incubated with rabbit anti-guinea-pig globulin serum. Comparable amounts of anti-SRBC antibody injected separately had no enhancing effect. Anti-SRBC sera from which the cytophilic antibody activity had been removed were inactive. PEC-associated SRBC were usually more immunogenic than the same amount of `free' SRBC for the induction of delayed hypersensitivity and always more immunogenic for antibody production.

These observations suggested that macrophage cytophilic antibody might be involved in the regulation of the immune response and play a role in the preferential induction of delayed hypersensitivity in the studied system.

Full text

PDF
469

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asherson G. L., Allison A. C., Zembala M. Production of delayed hypersensitivity by antigen associated with peritoneal exudate cells and the effect of pretreatment with Freund's complete adjuvant. Immunology. 1972 Mar;22(3):465–473. [PMC free article] [PubMed] [Google Scholar]
  2. BLOCH H., NORDIN A. A. Production of tuberculin sensitivity. Nature. 1960 Jul 30;187:434–435. doi: 10.1038/187434a0. [DOI] [PubMed] [Google Scholar]
  3. BOYDEN S. V. CYTOPHILIC ANTIBODY IN GUINEA-PIGS WITH DELAYED-TYPE HYPERSENSITIVITY. Immunology. 1964 Jul;7:474–483. [PMC free article] [PubMed] [Google Scholar]
  4. Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  5. Cruchaud A., Unanue E. R. Fate and immunogenicity of antigens endocytosed by macrophages: a study using foreign red cells and immunoglobulin G. J Immunol. 1971 Nov;107(5):1329–1340. [PubMed] [Google Scholar]
  6. Davey M. J., Asherson G. L., Stone S. H. Selective and specific inhibition of 24 hour skin reactions in the guinea-pig. 3. Depression of cytophilic and haemolytic antibodies by pretreatment with antigen and the effect of irradiation. Immunology. 1971 Apr;20(4):513–522. [PMC free article] [PubMed] [Google Scholar]
  7. Hoffmann M. Peritoneal macrophages in the immune response to SRBC in vitro. Immunology. 1970 Jun;18(6):791–797. [PMC free article] [PubMed] [Google Scholar]
  8. Howard J. G., Benacerraf B. Properties of macrophage receptors for cytophilic antibodies. Br J Exp Pathol. 1966 Apr;47(2):193–200. [PMC free article] [PubMed] [Google Scholar]
  9. Kunin S., Shearer G. M., Globerson A., Feldman M. Immunogenic function of macrophages: in vitro production of antibodies to a hapten-carrier conjugate. Cell Immunol. 1972 Oct;5(2):288–295. doi: 10.1016/0008-8749(72)90054-8. [DOI] [PubMed] [Google Scholar]
  10. Liew F. Y., Parish C. R. Regulation of the immune response by antibody. I. Suppression of antibody formation and concomitant enhancement of cell-mediated immunity by passive antibody. Cell Immunol. 1972 May;4(1):66–85. doi: 10.1016/0008-8749(72)90006-8. [DOI] [PubMed] [Google Scholar]
  11. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  12. Mitchison N. A. The immunogenic capacity of antigen taken up by peritoneal exudate cells. Immunology. 1969 Jan;16(1):1–14. [PMC free article] [PubMed] [Google Scholar]
  13. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  14. PAPPENHEIMER A. M., SALVIN S. B., UHR J. W. Delayed hypersensitivity. II. Induction of hypersensitivity in guinea pigs by means of antigen-antibody complexes. J Exp Med. 1957 Jan 1;105(1):11–24. doi: 10.1084/jem.105.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pearson M. N., Raffel S. Macrophage-digested antigen as inducer of delayed hypersensitivity. J Exp Med. 1971 Mar 1;133(3):494–505. doi: 10.1084/jem.133.3.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Seeger R. C., Oppenheim J. J. Macrophage-bound antigens. I. Induction of delayed hypersensitivity and priming for production of serum antibodies in guinea pigs. J Immunol. 1972 Aug;109(2):244–254. [PubMed] [Google Scholar]
  17. Seeger R. C., Oppenheim J. J. Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes. J Exp Med. 1970 Jul 1;132(1):44–65. doi: 10.1084/jem.132.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shortman K., Palmer J. The requirement for macrophages in the in vitro immune response. Cell Immunol. 1971 Oct;2(5):399–410. doi: 10.1016/0008-8749(71)90051-7. [DOI] [PubMed] [Google Scholar]
  19. Unanue E. R., Cerottini J. C., Bedford M. Persistence of antigen on the surface of macrophages. Nature. 1969 Jun 21;222(5199):1193–1195. doi: 10.1038/2221193a0. [DOI] [PubMed] [Google Scholar]
  20. Wagner H., Feldmann M., Boyle W., Schrader J. W. Cell-mediated immune response in vitro. 3. The requirement for macrophages in cytotoxic reactions against cell-bound and subcellular alloantigens. J Exp Med. 1972 Aug 1;136(2):331–343. doi: 10.1084/jem.136.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Waterston R. H. Soluble antigen from sheep erythrocytes: preparation and antigenic properties. Immunology. 1970 Mar;18(3):431–435. [PMC free article] [PubMed] [Google Scholar]
  22. Zembala M., Asherson G. L. Contact sensitivity in the mouse. V. The role of macrophage cytophilic antibody in passive transfer and the effect of trypsin and anti-gamma globulin serum. Cell Immunol. 1970 Sep;1(3):276–289. doi: 10.1016/0008-8749(70)90049-3. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES