Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1963 Sep;6(5):484–498.

A Factor Capable of Increasing Vascular Permeability Present in Lymph Node Cells

A Possible Mediator of the Delayed Reaction

D A Willoughby, Barbara Boughton, H O Schild
PMCID: PMC1423277  PMID: 14069726

Abstract

A soluble extract from guinea-pig lymph node cells (LPF) has been found to increase vascular permeability in the skin of the rat. The active substance has been differentiated from histamine, 5-hydroxytryptamine, bradykinin, substance P, kallikrein and the globulin permeability factors from rat and guinea-pig serum by means of parallel quantitative assays. LPF was present in both sensitized and non-sensitized guinea-pig lymph node cells and in lymph node cells from rats and mice. LPF also increased vascular permeability in the skin of guinea-pigs, mice and rabbits. The possible importance of this factor in the mechanism of the delayed reactions is discussed.

Full text

PDF
490

Images in this article

487FIG. 1
on p.487
489FIG. 3
on p.489

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOUGHTON B., SCHILD H. O. Transfer of delayed hypersensitivity by lymph-node cells in testis autosensitization. Immunology. 1962 Jul;5:522–532. [PMC free article] [PubMed] [Google Scholar]
  2. BOURA A., MONGAR J. L., SCHILD H. O. Improved automatic apparatus for pharmacological assays on isolated preparations. Br J Pharmacol Chemother. 1954 Mar;9(1):24–30. doi: 10.1111/j.1476-5381.1954.tb00811.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CROWLE A. J. Tuberculin skin reactions in mice hypersensitized by vaccination with living avirulent tubercle bacilli. Am Rev Respir Dis. 1960 Jun;81:893–903. doi: 10.1164/arrd.1960.81.6.893. [DOI] [PubMed] [Google Scholar]
  4. FISHER J. P., COOKE R. A. Experimental toxic and allergic contact dermatitis. I. A chemical study of histamine content. J Allergy. 1958 Sep;29(5):396–410. doi: 10.1016/0021-8707(58)90082-0. [DOI] [PubMed] [Google Scholar]
  5. FLAX M. H., WAKSMAN B. H. Delayed cutaneous reactions in the rat. J Immunol. 1962 Oct;89:496–504. [PubMed] [Google Scholar]
  6. HOLDSTOCK D. J., MATHIAS A. P., SCHACHTER M. A comparative study of kinin, kallidin, and bradykinin. Br J Pharmacol Chemother. 1957 Jun;12(2):149–158. doi: 10.1111/j.1476-5381.1957.tb00113.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. INDERBITZIN T. The mechanisms involved in histamine release and histamine increase in allergic skin reactions. Int Arch Allergy Appl Immunol. 1961;18:85–99. doi: 10.1159/000229154. [DOI] [PubMed] [Google Scholar]
  8. JOHANOVSKY J. Production of pyrogenic substances by extracts of delayed hypersensitive cells mixed with antigen in vitro. Nature. 1961 Apr 22;190:355–356. doi: 10.1038/190355a0. [DOI] [PubMed] [Google Scholar]
  9. MANNICK J. A., EGDAHL R. H. Ribonucleic acid in "transformation" of lymphoid cells. Science. 1962 Sep 21;137(3534):976–977. doi: 10.1126/science.137.3534.976. [DOI] [PubMed] [Google Scholar]
  10. METAXAS M. N., METAXAS-BUEHLER M. Studies on the cellular transfer of tuberculin sensitivity in the guinea pig. J Immunol. 1955 Nov;75(5):333–347. [PubMed] [Google Scholar]
  11. MONGAR J. L., SCHILD H. O. Inhibition of the anaphylactic reaction. J Physiol. 1957 Feb 15;135(2):301–319. doi: 10.1113/jphysiol.1957.sp005712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NAJARIAN J. S., FELDMAN J. D. Passive transfer of tuberculin sensitivity by tritiated thymidine-labeled lymphoid cells. J Exp Med. 1961 Nov 1;114:779–790. doi: 10.1084/jem.114.5.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SCHAYER R. W., GANLEY O. H. Adaptive increase in mammalian histidine decarboxylase activity in response to nonspecific stress. Am J Physiol. 1959 Sep;197:721–724. doi: 10.1152/ajplegacy.1959.197.3.721. [DOI] [PubMed] [Google Scholar]
  14. TURK J. L. The passive transfer of delayed hypersensitivity in guinea pigs by the transfusion of isotopically-labelled lymphoid cells. Immunology. 1962 Jul;5:478–488. [PMC free article] [PubMed] [Google Scholar]
  15. WILHELM D. L., MILES A. A., MACKAY M. E. Enzyme-like globulins from serum reproducing the vascular phenomena of inflammation. II. Isolation and properties of the permeability factor and its inhibitor. Br J Exp Pathol. 1955 Feb;36(1):82–104. [PMC free article] [PubMed] [Google Scholar]
  16. WILLOUGHBY D. A., BOUGHTON B., SPECTOR W. G., SCHILD H. O. A vascular permeability factor extracted from normal and sensitized guinea-pig lymph node cells. Life Sci. 1962 Jul;1:347–352. doi: 10.1016/0024-3205(62)90131-5. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES