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. 1963 Jul;6(4):382–402.

Induction of Autoimmunity to Adrenal Gland

E V Barnett, D C Dumonde, L E Glynn
PMCID: PMC1423324  PMID: 14042904

Abstract

Rabbits that were immunized with homologous and heterologous adrenal homogenates in complete adjuvant responded with complement-fixing heat-stable autoantibody primarily directed against adrenal. Rabbits responded with autoantibody production against cytoplasm of adrenal cortex and of ovarian and testicular cells as detected by immunofluorescence. Antibody, with sedimentation similar to 7S γ globulin, was directed against a heat-labile antigen in rabbit, guinea-pig and rat adrenal. Antibody responsible for reactions with the antigens in the complement fixation and immunofluorescent tests was absorbed by an ultra-centrifugal sediment of adrenal or ovary.

Histological evidence of adrenalitis was present only in those rabbits immunized with heterologous adrenal.

Guinea-pigs immunized with heterologous adrenal homogenates developed more extensive adrenal infiltrates than did guinea-pigs immunized with homologous adrenal. Antibody from guinea-pigs immunized with heterologous adrenal reacted with autologous organ antigens as well as with rabbit and rat adrenal. Skin tests with homologous adrenal revealed no evidence for delayed-type hypersensitivity.

The detection of autoantigens in foetal rabbit adrenal and ovary suggests that rabbits should be tolerant to such antigens. The greater efficacy of immunization with heterologous adrenal in eliciting autoantibody and autoimmune adrenalitis may be a feature of experimental autoimmunity induced by those organ-specific antigens to which the animal is tolerant. The presence of autoantibody in the serum, bound γ globulin at the site of adrenal lesions and the absence of delayed-type hypersensitivity to homologous adrenal suggest that the humoral immune mechanisms play a role in experimental autoimmune adrenalitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON J. R., GOUDIE R. B., GRAY K. G., TIMBURY G. C. Auto-antibodies in Addison's disease. Lancet. 1957 Jun 1;272(6979):1123–1124. doi: 10.1016/s0140-6736(57)91687-2. [DOI] [PubMed] [Google Scholar]
  2. ASHERSON G. L., DUMONDE D. C. Characterization of auto-antibodies produced in the rabbit by the injection of rat liver. Br J Exp Pathol. 1962 Feb;43:12–20. [PMC free article] [PubMed] [Google Scholar]
  3. BLIZZARD R. M., CHANDLER R. W., KYLE M. A., HUNG W. Adrenal antibodies in Addison's disease. Lancet. 1962 Nov 3;2(7262):901–903. doi: 10.1016/s0140-6736(62)90681-5. [DOI] [PubMed] [Google Scholar]
  4. COLOVER J., GLYNN L. E. Experimental iso-immune adrenalitis. Immunology. 1958 Apr;1(2):172–178. [PMC free article] [PubMed] [Google Scholar]
  5. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DIXON F. J., MAURER P. H. Specificity of the secondary response to protein antigens. J Immunol. 1955 Jun;74(6):418–431. [PubMed] [Google Scholar]
  7. DIXON F. J., VAZQUEZ J. J., WEIGLE W. O., COCHRANE C. G. Pathogenesis of serum sickness. AMA Arch Pathol. 1958 Jan;65(1):18–28. [PubMed] [Google Scholar]
  8. DONNELLEY M. Studies in experimental immunology of influenza. VII. An improved complement-fixation technique. Aust J Exp Biol Med Sci. 1951 Mar;29(2):137–146. doi: 10.1038/icb.1951.16. [DOI] [PubMed] [Google Scholar]
  9. EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FREUND J., LIPTON M. M., THOMPSON G. E. Aspermatogenesis in the guinea pig induced by testicular tissue and adjuvants. J Exp Med. 1953 May;97(5):711–726. doi: 10.1084/jem.97.5.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FREUND J., THOMPSON G. E., LIPTON M. M. Aspermatogenesis, anaphylaxis, and cutaneous sensitization induced in the guinea pig by homologous testicular extract. J Exp Med. 1955 Jun 1;101(6):591–604. doi: 10.1084/jem.101.6.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GELL P. G., BENACERRAF B. Studies on hypersensitivity. IV. The relationship between contact and delayed sensitivity: a study of the specificity of cellular immune reactions. J Exp Med. 1961 Mar 1;113:571–585. doi: 10.1084/jem.113.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. GERY I., DAVIES A. M. Organ specificity of the heart. I. Animal immunization with heterologous heart. J Immunol. 1961 Oct;87:351–356. [PubMed] [Google Scholar]
  14. KAPLAN M. H., MEYESERIAN M. Immunologic studies of heart tissue. V. Antigens related to heart tissue revealed by cross-reaction of rabbit antisera to heterologous heart. J Immunol. 1962 Apr;88:450–461. [PubMed] [Google Scholar]
  15. MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
  16. MCMASTER P. R., LERNER E. M., 2nd, EXUM E. D. The relationship of delayed hypersensitivity and circulating antibody to experimental allergic thyroditis in inbred guinea pigs. J Exp Med. 1961 Apr 1;113:611–624. doi: 10.1084/jem.113.4.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MEAD R. K. Autoimmune Addison's disease. Report of a possible case. N Engl J Med. 1962 Mar 22;26:583–586. [PubMed] [Google Scholar]
  18. MIESCHER P., GORSTEIN F., BENACERRAF B., GELL P. G. Studies on the pathogenesis of experimental immune thyroiditis. Proc Soc Exp Biol Med. 1961 May;107:12–16. doi: 10.3181/00379727-107-26519. [DOI] [PubMed] [Google Scholar]
  19. MILGROM F., LUSZCZYNSKI T., DUBISKI S. Preparation of antiglobulin sera. Nature. 1956 Feb 18;177(4503):329–329. doi: 10.1038/177329a0. [DOI] [PubMed] [Google Scholar]
  20. MILGROM F., WITEBSKY E. Immunological studies on adrenal glands. I. Immunization with adrenals of foreign species. Immunology. 1962 Jan;5:46–66. [PMC free article] [PubMed] [Google Scholar]
  21. ROSE N. R., WITEBSKY E. Studies on organ specificity. VI. Cross-reactions of thyroid autoantibodies with thyroids of other species. J Immunol. 1959 Jul;83(1):34–40. [PubMed] [Google Scholar]
  22. SMITH C. W., MARSHALL J. D., Jr, EVELAND W. C. Use of contrasting fluorescent dye as counterstain in fixed tissue preparations. Proc Soc Exp Biol Med. 1959 Oct;102:179–181. doi: 10.3181/00379727-102-25182. [DOI] [PubMed] [Google Scholar]
  23. STEINER J. W., LANGER B., SCHATZ D. L. The local and systemic effects of Freund's adjuvant and its fractions. Arch Pathol. 1960 Oct;70:424–434. [PubMed] [Google Scholar]
  24. TEDESCHI L. G., PEABODY C. N. Cortical necrosis of the adrenal gland. Arch Pathol. 1962 Jan;73:6–12. [PubMed] [Google Scholar]
  25. WACKER W. B., DODD M. C. The immunologic response of rabbits to homologous and heterologous corneal and uveal tissue. J Immunol. 1961 Jun;86:690–703. [PubMed] [Google Scholar]
  26. WAKSMAN B. H., ADAMS R. D. Allergic neuritis: an experimental disease of rabbits induced by the injection of peripheral nervous tissue and adjuvants. J Exp Med. 1955 Aug 1;102(2):213–236. doi: 10.1084/jem.102.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WAKSMAN B. H., MORRISON L. R. Tuberculin type sensitivity to spinal cord antigen in rabbits with isoallergic encephalomyelitis. J Immunol. 1951 Apr;66(4):421–444. [PubMed] [Google Scholar]
  28. WEIGLE W. O. The immune response of rabbits tolerant to bovine serum albumin to the injection of other heterologous serum albumins. J Exp Med. 1961 Jul 1;114:111–125. doi: 10.1084/jem.114.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. WITEBSKY E., MILGROM F. Immunological studies on adrenal glands. II. Immunization with adrenals of the same species. Immunology. 1962 Jan;5:67–78. [PMC free article] [PubMed] [Google Scholar]

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