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. 1974 Jun;26(6):1093–1099.

Characterization of the blastogenic response of C58 spleen cells: age-dependent changes

J W M Lawton, W H Murphy
PMCID: PMC1423352  PMID: 4850984

Abstract

Optimum conditions were determined for the stimulation of DNA synthesis in C58 spleen cells by phytohaemagglutinin and allogeneic BALB spleen cells. Maximum stimulation occurred in 3 days in both cases. The rate of DNA synthesis was constant over a 4-hour labelling period with tritiated thymidine. DNA synthesis was significantly enhanced by 15 mM bicarbonate. The peak response of C58 spleen cells to PHA and allogeneic BALB spleen cells occurred when the mice were 3–7 months old; it decreased thereafter. This finding is discussed in relation to age-dependent immune polioencephalomyelitis in C58 mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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