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. 1964 Jul;7(4):440–448.

In vitro production of chicken globulins and precipitating antibody*

R Patterson, Irena M Suszko, J J Pruzansky
PMCID: PMC1423440  PMID: 14193156

Abstract

Immunofluorescent and isotope coprecipitation techniques provided methods of studying in vitro production of antibody by chicken spleen cells. In some culture preparations precipitating antibody was produced in sufficient quantity to be measured by quantitative techniques. The in vitro production of globulins by chicken spleen cells has been demonstrated by paper and immunoelectrophoresis of tissue culture medium. The antibody in the globulin produced in the in vitro tissue culture preparations may be shown by an autoradiograph of the immunoelectrophoresis after the addition of antigen trace labelled with radioactive iodine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  2. ORLANS E. Fowl antibody: II. The composition of specific precipitates formed by antisera to serum albumin, haemoglobin and myoglobin and some properties of non-precipitating antibody. Immunology. 1962 Mar;5:306–321. [PMC free article] [PubMed] [Google Scholar]
  3. PATTERSON R., SUSZKO I. M. IN VITRO PRODUCTION OF ANTIBODY BY CHICKEN SPLEEN CELLS. II. IMMUNOFLUORESCENT STUDIES OF TISSUE CULTURE PREPARATIONS. J Immunol. 1963 Jun;90:836–842. [PubMed] [Google Scholar]
  4. PATTERSON R., SUSZKO I. M., PRUZANSKY J. J. IN VITRO PRODUCTION OF ANTIBODY BY CHICKEN SPLEEN CELLS. I. DETECTION OF ANTIBODY BY ISOTOPE TECHNIQUES. J Immunol. 1963 Jun;90:829–835. [PubMed] [Google Scholar]
  5. TALMAGE D. W., BAKER H. R., AKESON W. The separation and analysis of labelled antibodies. J Infect Dis. 1954 Mar-Apr;94(2):199–212. doi: 10.1093/infdis/94.2.199. [DOI] [PubMed] [Google Scholar]
  6. TALMAGE D. W., MAURER P. H. I131-Labelled antigen precipitation as a measure of quantity and quality of antibody. J Infect Dis. 1953 May-Jun;92(3):288–300. doi: 10.1093/infdis/92.3.288. [DOI] [PubMed] [Google Scholar]

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