Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1966 Apr;10(4):383–397.

In vitro synthesis of γM-like globulin by various chick embryonic cells*

V A Marinkovich, M A Baluda
PMCID: PMC1423666  PMID: 4160446

Abstract

Mass cultures of cells derived from bursa of Fabricius, yolk sac and thigh muscle of 16-day chick embryos, and from the peripheral blood of adult chickens, and cloned chick embryonic muscle cells synthesize and release into the nutrient medium a protein which has the antigenic and physico-chemical characteristics of chicken γM-globulin.

Full text

PDF
397

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABDEL-SAMIE Y. M., BRODA E., KELLNER G. The autonomous production of individual serum proteins by tissue in cluture. Biochem J. 1960 May;75:209–215. doi: 10.1042/bj0750209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ABDEL-SAMIE Y., BRODA E., KELLNER G., ZISCHKA W. Production of serum albumin and of globulins by chick mesenchymal tissue and by HeLa tumour tissue in culture. Nature. 1959 Aug 1;184(Suppl 6):361–362. doi: 10.1038/184361a0. [DOI] [PubMed] [Google Scholar]
  3. ADA G. L., NOSSAL G. J., PYE J., ABBOT A. BEHAVIOUR OF ACTIVE BACTERIAL ANTIGENS DURING THE INDUCTION OF THE IMMUNE RESPONSE. I. PROPERTIES OF FLAGELLAR ANTIGENS FROM SALMONELLA. Nature. 1963 Sep 28;199:1257–1259. doi: 10.1038/1991257a0. [DOI] [PubMed] [Google Scholar]
  4. ASKONAS B. A., HUMPHREY J. H., PORTER R. R. On the origin of the multiple forms of rabbit gamma-globulin. Biochem J. 1956 Jul;63(3):412–419. doi: 10.1042/bj0630412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BALUDA M. A., GOETZ I. E. Morphological conversion of cell cultures by avian myeloblastosis virus. Virology. 1961 Oct;15:185–199. doi: 10.1016/0042-6822(61)90234-3. [DOI] [PubMed] [Google Scholar]
  6. BRAKKE M. K. Zonal separations by density-gradient centrifugation. Arch Biochem Biophys. 1953 Aug;45(2):275–290. doi: 10.1016/s0003-9861(53)80005-6. [DOI] [PubMed] [Google Scholar]
  7. BUDZYNSKI A. Z., BRODA E., KELLNER G., FRIMMEL J. S. An examination of HeLa cancer cells for production of gamma-globulin. Nature. 1962 Dec 1;196:892–893. doi: 10.1038/196892a0. [DOI] [PubMed] [Google Scholar]
  8. Bauer D. C., Stavitsky A. B. ON THE DIFFERENT MOLECULAR FORMS OF ANTIBODY SYNTHESIZED BY RABBITS DURING THE EARLY RESPONSE TO A SINGLE INJECTION OF PROTEIN AND CELLULAR ANTIGENS. Proc Natl Acad Sci U S A. 1961 Oct;47(10):1667–1680. doi: 10.1073/pnas.47.10.1667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Danielsson C. E. Seed globulins of the Gramineae and Leguminosae. Biochem J. 1949;44(4):387–400. doi: 10.1042/bj0440387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. EAGLE H., PIEZ K. A., FLEISCHMAN R., OYAMA V. I. Protein turnover in mammaliar cell cultures. J Biol Chem. 1959 Mar;234(3):592–597. [PubMed] [Google Scholar]
  11. FINKELSTEIN M. S., UHR J. W. SPECIFIC INHIBITION OF ANTIBODY FORMATION BY PASSIVELY ADMINISTERED 19S AND 7S ANTIBODY. Science. 1964 Oct 2;146(3640):67–69. doi: 10.1126/science.146.3640.67. [DOI] [PubMed] [Google Scholar]
  12. FLEISCHMAN J. B. THE SYNTHESIS OF GAMMA-GLOBULIN BY RABBIT LYMPH NODE CELLS. J Immunol. 1963 Aug;91:163–169. [PubMed] [Google Scholar]
  13. GRABAR P., WILLIAMS C. A., Jr Méthode immuno-électrophorétique d'analyse de mélanges de substances antigéniques. Biochim Biophys Acta. 1955 May;17(1):67–74. doi: 10.1016/0006-3002(55)90320-6. [DOI] [PubMed] [Google Scholar]
  14. HELMREICH E., KERN M., EISEN H. N. Observations on the mechanism of secretion of gamma-globulins by isolated lymph node cells. J Biol Chem. 1962 Jun;237:1925–1931. [PubMed] [Google Scholar]
  15. HELMREICH E., KERN M., EISEN H. N. The secretion of antibody by isolated lymph node cells. J Biol Chem. 1961 Feb;236:464–473. [PubMed] [Google Scholar]
  16. KESTON A. S., KATCHEN B. Incorporation of glycine-2-C14 into homologous antibody by rabbit tissue slices. J Immunol. 1956 Apr;76(4):253–258. [PubMed] [Google Scholar]
  17. KUNKEL H. G., SLATER R. J. Zone electrophoresis in a starch supporting medium. Proc Soc Exp Biol Med. 1952 May;80(1):42–44. doi: 10.3181/00379727-80-19516. [DOI] [PubMed] [Google Scholar]
  18. LOSPALLUTO J., MILLER W., Jr, DORWARD B., FINK C. W. The formation of macroglobulin antibodies. I. Studies on adult humans. J Clin Invest. 1962 Jul;41:1415–1421. doi: 10.1172/JCI104596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MCFARLANE H. A SIMPLE RAPID METHOD OF CONCENTRATING URINE FOR PROTEIN ELECTROPHORESIS. Clin Chim Acta. 1964 Apr;9:376–380. doi: 10.1016/0009-8981(64)90029-4. [DOI] [PubMed] [Google Scholar]
  20. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  21. STAVITSKY A. B. In vitro production of diphtheria antitoxin by tissues of immunized animals. III. Incorporation of amino acids into antibody; relationship to antibody synthesis and sensitivity relative to other methods. Br J Exp Pathol. 1958 Dec;39(6):661–673. [PMC free article] [PubMed] [Google Scholar]
  22. TALMAGE D. W., FRETER G. G., TALIAFERRO W. H. Two antibodies of related specificity but different hemolytic efficiency separated by centrifugation. J Infect Dis. 1956 May-Jun;98(3):300–305. doi: 10.1093/infdis/98.3.300. [DOI] [PubMed] [Google Scholar]
  23. UHR J. W., FINKELSTEIN S., FRANKLIN E. C. Antibody response to bacteriophage phi-X-174 in non-mammalian vertebrates. Proc Soc Exp Biol Med. 1962 Oct;111:13–15. doi: 10.3181/00379727-111-27691. [DOI] [PubMed] [Google Scholar]
  24. WALLENIUS G., TRAUTMAN R., KUNKEL H. G., FRANKLIN E. C. Ultracentrifugal studies of major non-lipide electrophoretic components of normal human serum. J Biol Chem. 1957 Mar;225(1):253–267. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES