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. 1966 Apr;10(4):309–319.

Isolation of purified H and L polypeptide chains from guinea-pig γ2-immunoglobulin after mild reduction

M E Lamm, V Nussenzweig, B Benacerraf
PMCID: PMC1423667  PMID: 4160443

Abstract

A method is described whereby biologically active H and L chains, free from each other by immunochemical criteria, can be prepared from guinea-pig γ2-immunoglobulin. The procedure makes use of mild reduction and alkylation in the absence of a denaturing agent, followed by gel filtration in the presence of 4 M guanidine·HCl.

Specific binding of antigen by the method of equilibrium dialysis was found only when specific H chains were mixed with L chains, with more activity resulting when the L chains were also specific. Specific binding by antibody H chains alone was not found.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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