Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1966 Jun;10(6):517–526.

Serological properties of γA antibodies to Escherichia coli present in human colostrum

M Adinolfi, A A Glynn, Margaret Lindsay, Celia M Milne
PMCID: PMC1423745  PMID: 5330427

Abstract

Antibodies against Esch. coli WF 96 and WF 61 present in human colostrum and serum were fractionated by DEAE-cellulose chromatography. Using the haemagglutination test it was found that the antibodies present in colostrum were recovered in the fraction containing the bulk of γA-globulin, whereas the antibodies present in serum were recovered in the fraction containing the bulk of γM-globulin. In the presence of human or guinea-pig complement the antibodies present in colostrum did not lyse red cells coated with bacterial polysaccharides whereas the antibodies present in serum were lytic.

When the properties of γA and γM antibodies were studied using a bacteriolytic system, it was observed that γA-globulin lysed bacteria only in the presence of both complement and lysozyme; in this respect γAbacterial antibodies behaved differently from γM antibodies which were bacteriolytic in the presence of complement alone, without lysozyme.

The effect of treating γA and γM antibodies with 2-mercaptoethanol at neutral pH and of heating at 56° was investigated.

Full text

PDF
521

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASKONAS B. A., CAMPBELL P. N., HUMPHREY J. H., WORK T. S. The source of antibody globulin in rabbit milk and goat colostrum. Biochem J. 1954 Apr;56(4):597–601. [PMC free article] [PubMed] [Google Scholar]
  2. Adinolfi M. Some serological characteristics of normal incomplete cold antibody. Immunology. 1965 Jul;9(1):31–42. [PMC free article] [PubMed] [Google Scholar]
  3. CHANDAN R. C., SHAHANI K. M., HOLLY R. G. LYSOZYME CONTENT OF HUMAN MILK. Nature. 1964 Oct 3;204:76–77. doi: 10.1038/204076a0. [DOI] [PubMed] [Google Scholar]
  4. CHODIRKER W. B., TOMASI T. B., Jr GAMMA-GLOBULINS: QUANTITATIVE RELATIONSHIPS IN HUMAN SERUM AND NONVASCULAR FLUIDS. Science. 1963 Nov 22;142(3595):1080–1081. doi: 10.1126/science.142.3595.1080. [DOI] [PubMed] [Google Scholar]
  5. FERRARI R., MATRACIA S. Interaction between lysozyme and a phospholipid of neoplastic tissue (oncolipin). Nature. 1961 Dec 23;192:1187–1187. doi: 10.1038/1921187a0. [DOI] [PubMed] [Google Scholar]
  6. FLANAGAN P., LIONETTI F. Lysozyme distribution in blood. Blood. 1955 May;10(5):497–501. [PubMed] [Google Scholar]
  7. GLYNN A. A., PARKMAN R. STUDIES WITH AN ANTIBODY TO RAT LYSOZYME. Immunology. 1964 Nov;7:724–729. [PMC free article] [PubMed] [Google Scholar]
  8. Glynn A. A., Milne C. M. Lysozyme and immune bacteriolysis. Nature. 1965 Sep 18;207(5003):1309–1310. doi: 10.1038/2071309a0. [DOI] [PubMed] [Google Scholar]
  9. HODES H. I., CORIELL L. L. POLIOMYELITIS ANTIBODIES IN HUMAN COLOSTRUM AND MILK. J Pediatr. 1964 Aug;65:319–320. doi: 10.1016/s0022-3476(64)80540-0. [DOI] [PubMed] [Google Scholar]
  10. LANDY M., MICHAEL J. G., WHITBY J. L. Bactericidal method for the measurement in normal serum of antibody to gramnegative bacteria. J Bacteriol. 1962 Mar;83:631–640. doi: 10.1128/jb.83.3.631-640.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MUSCHEL L. H., CAREY W. F., BARON L. S. Formation of bacterial protoplasts by serum components. J Immunol. 1959 Jan;82(1):38–42. [PubMed] [Google Scholar]
  12. NETER E. Bacterial hemagglutination and hemolysis. Bacteriol Rev. 1956 Sep;20(3):166–188. doi: 10.1128/br.20.3.166-188.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PIKE R. M., SCHULZE M. L. THE RELATIVE HEAT STABILITY OF ANTIBODIES IN CHROMATOGRAPHIC FRACTIONS OF RABBIT ANTISERA TO VARIOUS ANTIGENS. J Immunol. 1965 Jan;94:31–36. [PubMed] [Google Scholar]
  14. POLLEY M. J., MOLLISON P. L., SOOTHILL J. F. The role of 19S gamma-globulin blood-group antibodies in the antiglobulin reaction. Br J Haematol. 1962 Apr;8:149–162. doi: 10.1111/j.1365-2141.1962.tb06507.x. [DOI] [PubMed] [Google Scholar]
  15. WARDLAW A. C. The complement-dependent bacteriolytic activity of normal human serum. I. The effect of pH and ionic strength and the role of lysozyme. J Exp Med. 1962 Jun 1;115:1231–1249. doi: 10.1084/jem.115.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES