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. 1966 Sep;11(3):229–241.

The role of mycobacteria and silica in the immunological response of the guinea-pig

P C Wilkinson, R G White
PMCID: PMC1423776  PMID: 4162436

Abstract

The relationships between the development of a local epithelioid or macrophage granulomatous tissue reaction, of delayed-type hypersensitivity and of biosynthesis of γ2-immunoglobulin have been explored in guinea-pigs injected with antigen mixtures containing aluminium silicate (bentonite) particles in saline suspension or containing whole mycobacterial cells or their extracted peptidoglycolipids in water-in-oil emulsions.

Bentonite was found to be effective in inducing local granulomatous tissue proliferation and the formation of γ2-immunoglobulin. In the same animals, in a minority of circumstances, bentonite was capable of inducing delayed-type hypersensitivity to the accompanying protein antigen. Thus bentonite could reproduce, in these three respects, the specific immunological effects produced by mycobacteria or their extracted peptidoglycolipids. By the use of prior injection of the homologous antigen, partial blockage of the production of delayed-type hypersensitivity following subsequent injection of the antigen in Freund's complete adjuvant could be effected. At the same time changes occurred in granuloma formation and biosynthesis of γ2-immunoglobulin. The reasons for the apparent linkage of these events are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., White R. G., Wilkinson P. C. Production of gamma-1- and gamma-2-antiovalbumin by various lymphoid tissues of the guinea pig. Immunochemistry. 1965 Dec;2(4):329–336. doi: 10.1016/0019-2791(65)90033-9. [DOI] [PubMed] [Google Scholar]
  2. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOYDEN S. V. The effect of previous injections of tuberculoprotein on the development of tuberculin sensitivity following B.C.G. vaccination in guinea-pigs. Br J Exp Pathol. 1957 Dec;38(6):611–617. [PMC free article] [PubMed] [Google Scholar]
  4. BOZICEVICH J., BUNIM J. J., FREUND J., WARD S. B. Bentonite flocculation test for rheumatoid arthritis. Proc Soc Exp Biol Med. 1958 Jan;97(1):180–183. doi: 10.3181/00379727-97-23681. [DOI] [PubMed] [Google Scholar]
  5. FISCHEL E. E., KABAT E. A., STOERK H. C., BEZER A. E. The role of tubercle bacilli in adjuvant emulsions on antibody production to egg albumin. J Immunol. 1952 Dec;69(6):611–618. [PubMed] [Google Scholar]
  6. FISHMAN M., HAMMERSTROM R. A., BOND V. P. In vitro transfer of macrophage RNA to lymph node cells. Nature. 1963 May 11;198:549–551. doi: 10.1038/198549a0. [DOI] [PubMed] [Google Scholar]
  7. PERNIS B., PARONETTO F. Adjuvant effect of silica (tridymite) on antibody production. Proc Soc Exp Biol Med. 1962 Jun;110:390–392. doi: 10.3181/00379727-110-27527. [DOI] [PubMed] [Google Scholar]
  8. RHODES M. B., AZARI P. R., FEENEY R. E. Analysis, fractionation, and purification of egg white proteins with cellulose-cation exchanger. J Biol Chem. 1958 Jan;230(1):399–408. [PubMed] [Google Scholar]
  9. SUTER E., WHITE R. G. The response of the reticulo-endothelial system to the injection of the purified wax and the lipopolysaccharide of tubercle bacilli; a histologic and an immunologic study. Am Rev Tuberc. 1954 Nov;70(5):793–805. doi: 10.1164/art.1954.70.5.793. [DOI] [PubMed] [Google Scholar]
  10. Stewart-Tull D. E., Wilkinson P. C., White R. G. The affinity of a mycobacterial glycopeptide for guinea-pig gamma-globulin. Immunology. 1965 Aug;9(2):151–160. [PMC free article] [PubMed] [Google Scholar]
  11. WHITE R. G., COONS A. H., CONNOLLY J. M. Studies on antibody production. IV. The role of a wax fraction of Mycobacterium tuberculosis in adjuvant emulsions on the production of antibody to egg albumin. J Exp Med. 1955 Jul 1;102(1):83–104. doi: 10.1084/jem.102.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WHITE R. G., JENKINS G. C., WILKINSON P. C. The production of skin-sensitizing antibody in the guinea-pig. Int Arch Allergy Appl Immunol. 1963;22:156–165. doi: 10.1159/000229362. [DOI] [PubMed] [Google Scholar]
  13. WHITE R. G., JOLLES P., SAMOUR D., LEDERER E. CORRELATION OF ADJUVANT ACTIVITY AND CHEMICAL STRUCTURE OF WAX D FRACTIONS OF MYCOBACTERIA. Immunology. 1964 Mar;7:158–171. [PMC free article] [PubMed] [Google Scholar]
  14. WHITE R. G., MARSHALL A. H. The role of various chemical fractions of M. tuberculosis and other Mycobacteria in the production of allergic encephalomyelitis. Immunology. 1958 Apr;1(2):111–122. [PMC free article] [PubMed] [Google Scholar]

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