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. 1966 Nov;11(5):475–488.

Antigen localization in lymphopenic states

II. Further studies on whole body X-irradiation

G M Williams
PMCID: PMC1423798  PMID: 5926068

Abstract

The gross and microscopic distribution of 125I polymerized flagellin from Salmonella adelaide was studied in adult rats at various times following 800 r whole body X-irradiation. Injections of radioactive antigen were made in both hind footpads, and the popliteal lymph nodes were excised for autoradiographic study 1 day later. This dose of irradiation caused a progressive decline in the ability of lymphoid follicles of popliteal nodes to capture and retain antigen. Irradiation had no detectable effect upon antigen uptake by whole lymph nodes or upon the number of grains overlying the phagocytic cells of the medullary sinuses of popliteal nodes.

Various substances capable of restoring follicular antigen uptake in the irradiated rat were studied by means of injecting the test substance into one hind footpad 1 hour prior to the injection of antigen into both feet. The distribution of antigen in each popliteal node was compared, each animal thus acting as its own control. It was found that 0.01 ml of specific anti-flagellar immune serum, or 0.25 ml of normal adult rat serum significantly improved follicular antigen uptake when tested ten days after irradiation. Foetal calf serum, homologous lymphocytes, and the media from pooled concentrated lymphocyte cultures were without demonstrable effects when given by regional injection. Shielding of the popliteal nodes at the time of irradiation improved follicular antigen uptake, whereas shielding of the femoral bone marrow and appendix was ineffective. No agent found capable of improving follicular antigen capture in the irradiated rat significantly altered footpad retention of antigen, whole organ counts of the popliteal nodes, or antigen localization in the phagocytic cells of the lymph node medulla.

The results favour the interpretation that the follicular antigen trapping mechanism is extremely sensitive to changes in levels of opsonins; that substances present in normal adult rat serum act as `follicular opsonins'; that these substances decline exponentially following irradiation; and that these substances are secreted by small lymphocytes or their progeny.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADA G. L., NOSSAL G. J., PYE J., ABBOT A. ANTIGENS IN IMMUNITY. I. PREPARATION AND PROPERTIES OF FLAGELLAR ANTIGENS FROM SALMONELLA ADELAIDE. Aust J Exp Biol Med Sci. 1964 Jun;42:267–282. [PubMed] [Google Scholar]
  2. ARCHER O. K., SUTHERLAND D. E., GOOD R. A. APPENDIX OF THE RABBIT: A HOMOLOGUE OF THE BURSA IN THE CHICKEN? Nature. 1963 Oct 26;200:337–339. doi: 10.1038/200337a0. [DOI] [PubMed] [Google Scholar]
  3. DIXON F. J., McCONAHEY P. J. Enhancement of antibody formation by whole body x-radiation. J Exp Med. 1963 May 1;117:833–848. doi: 10.1084/jem.117.5.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HALL J. G., MORRIS B. EFFECT OF X-IRRADIATION OF THE POPLITEAL LYMPH-NODE ON ITS OUTPUT OF LYMPHOCYTES AND IMMUNOLOGICAL RESPONSIVENESS. Lancet. 1964 May 16;1(7342):1077–1080. doi: 10.1016/s0140-6736(64)91274-7. [DOI] [PubMed] [Google Scholar]
  5. JACOBSON L. O., SIMMONS E. L., MARKS E. K., ROBSON M. J., BETHARD W. F., GASTON E. O. The role of the spleen in radiation injury and recovery. J Lab Clin Med. 1950 May;35(5):746–770. [PubMed] [Google Scholar]
  6. KAHN R. L., KIM S. H., CURTIS A. C., SIMONS C. S. X irradiation and toxin neutralization by antitoxin. J Infect Dis. 1960 Nov-Dec;107:325–331. doi: 10.1093/infdis/107.3.325. [DOI] [PubMed] [Google Scholar]
  7. KORNFELD L., HAMMOND C. W., MILLER C. P. The effect of irradiation on natural bactericidins of mice. J Immunol. 1960 Jan;84:77–81. [PubMed] [Google Scholar]
  8. Mitchell J., Abbot A. Ultrastructure of the antigen-retaining reticulum of lymph node follicles as shown by high-resolution autoradiography. Nature. 1965 Oct 30;208(5009):500–502. doi: 10.1038/208500b0. [DOI] [PubMed] [Google Scholar]
  9. Nossal G. J., Ada G. L., Austin C. M., Pye J. Antigens in immunity. 8. Localization of 125-I-labelled antigens in the secondary response. Immunology. 1965 Oct;9(4):349–357. [PMC free article] [PubMed] [Google Scholar]
  10. SUSSDORF D. H. Repopulation of the spleen of x-irradiated rabbits by tritium-labeled lymphoid cells of the shielded appendix. J Infect Dis. 1960 Jul-Aug;107:108–114. doi: 10.1093/infdis/107.1.108. [DOI] [PubMed] [Google Scholar]
  11. TALMAGE D. W. Effect of ionizing radiation on resistance and infection. Annu Rev Microbiol. 1955;9:335–346. doi: 10.1146/annurev.mi.09.100155.002003. [DOI] [PubMed] [Google Scholar]
  12. Williams G. M. Antigen localization in lymphopenic states. I. Localization pattern following chronic thoracic duct drainage. Immunology. 1966 Nov;11(5):467–474. [PMC free article] [PubMed] [Google Scholar]

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