Abstract
The value of sera containing immuno-conglutinin or conglutinin in demonstrating complement-binding by human blood group iso-antibodies was investigated. Provided that a suitable complement was used, positive results were obtained with all human sera which had been shown by other methods to contain complement-binding antibodies.
When human red cells were sensitized with human complement-binding blood group antibody and then treated with horse serum as a source of complement, the strongest reactions were obtained with rabbit immuno-conglutinin; the reactions with bovine serum (containing conglutinin) were distinctly weaker, and the reactions with human sera containing immuno-conglutinin were weaker still. When rabbit complement was used instead of horse complement, stronger reactions were obtained, but it was difficult to avoid `false positive' reactions. When human complement was used, good reactions were obtained with rabbit immuno-conglutinin, but the reactions with bovine conglutinin and human immuno-conglutinin were completely negative.
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Selected References
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