Abstract
Asian strains of Pasteurella multocida Type I were extracted with saline to remove superficial material and then with phenol-water to extract the lipopolysaccharides. This product was recovered from the aqueous phase by centrifuging at 105,000 g for 2 hours. The component sugars are galactose, glucose and glucosamine and a heptose sugar, possibly D-glycero-L-mannoheptose. The preparation was toxic for rabbits and was absorbed on human erythrocytes.
An Australian strain of P. multocida, which by conventional mouse protection and haemagglutination tests was identified as Type I yielded a lipopolysaccharide with different properties. The disease of cattle, haemorrhagic septicaemia, which is caused by P. multocida Type I in Asia is unknown in Australia.
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Selected References
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