Abstract
The sera of CBA mice lethally irradiated and restored with rat bone marrow or foetal liver have been examined by immunoelectrophoresis at various times after irradiation. Such sera contain several specific rat proteins including γ globulin, at least two α2 globulins, one or two β1 globulins and one β2 globulin. A large number of specific mouse proteins are also present, but no mouse γ globulins have been detected.
The rat constituents appear progressively and persist for long periods; this implies that they are synthesized by rat cells.
There is a close correlation between the presence of rat γ globulin in the serum and the presence of dividing rat cells in the bone marrow and spleen.
The fact that γ globulin is solely of rat specificity does not necessarily rule out the possibility of immune activity by the host against the graft.
Several of the normal serum proteins, in addition to the γ globulins, originate from the injected cells, and are therefore presumably not synthesized by the liver.
In chimaeras grafted with rat skin, the number of specific rat serum proteins is often larger than the number in chimaeras which have not been grafted.
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- CORP M. J. Whole body x-irradiation of experimental animals; a short summary of the methods used at the M.R.C. Radiobiological Research Unit, A.E.R.E., Harwell, between 1949-1956. Phys Med Biol. 1957 Apr;1(4):370–379. doi: 10.1088/0031-9155/1/4/306. [DOI] [PubMed] [Google Scholar]
- FORD C. E., HAMERTON J. L., BARNES D. W., LOUTIT J. F. Cytological identification of radiation-chimaeras. Nature. 1956 Mar 10;177(4506):452–454. doi: 10.1038/177452a0. [DOI] [PubMed] [Google Scholar]
- FORD C. E., ILBERY P. L., LOUTIT J. F. Further cytological observations on radiation chimeras. J Cell Physiol Suppl. 1957 Dec;50(Suppl 1):109–121. doi: 10.1002/jcp.1030500408. [DOI] [PubMed] [Google Scholar]
- GENGOZIAN N., CARTER R., PETERSON W. J. Antibody synthesis by transplanted heterologous chimera spleen cells in irradiated recipients. J Immunol. 1961 Aug;87:209–217. [PubMed] [Google Scholar]
- GORER P. A. Some reactions of H-2 antibodies in vitro and in vivo. Ann N Y Acad Sci. 1958 Oct 7;73(3):707–721. doi: 10.1111/j.1749-6632.1959.tb40848.x. [DOI] [PubMed] [Google Scholar]
- GRABAR P., COURCON J. Etude des sérums de cheval, lapin, rat et souris par l'analyse immunoélectrophorétique. Bull Soc Chim Biol (Paris) 1958;40(12):1993–2003. [PubMed] [Google Scholar]
- GRABAR P., COURCON J., ILBERG P. L., LOUTIT J. F., MERRILL J. P. Etude immuno-électrophorétique du sérum de souris irradiées par des doses létales de rayons X et protégées par des cellules de la moelle osseuse de rats. C R Hebd Seances Acad Sci. 1957 Sep 2;245(10):950–952. [PubMed] [Google Scholar]
- GRABAR P., WILLIAMS C. A., Jr Méthode immuno-électrophorétique d'analyse de mélanges de substances antigéniques. Biochim Biophys Acta. 1955 May;17(1):67–74. doi: 10.1016/0006-3002(55)90320-6. [DOI] [PubMed] [Google Scholar]
- MICKLEM H. S. Survival of rat skin grafts on lethally irradiated CBA mice restored with CBA foetal liver. Transplant Bull. 1962 Jan;29:7–9. [PubMed] [Google Scholar]
- POPP R. A., SMITH L. H. Electrophoresis of proteins of irradiated mouse chimeras. J Natl Cancer Inst. 1959 Sep;23:395–409. [PubMed] [Google Scholar]
- ROSE N., PEETOOM F., RUDDY S., MICHELI A., GRABAR P. [Immunochemical study of hemolystates of human red cells. 1. Principle antigenic constituents]. Ann Inst Pasteur (Paris) 1960 Jan;98:70–89. [PubMed] [Google Scholar]