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. 1962 Sep;5(5):549–556.

Antigenic Analysis of Rheumatoid Factor

D S Rowe
PMCID: PMC1424167  PMID: 13982809

Abstract

Papain digestion of human γ globulin yields two main antigenic components, of slow (S) and fast (F) electrophoretic mobility. Recent work has shown that β2M globulin (γ1 macroglobulin) contains S but not F antigenic groupings. It also contains specific groupings which may be called X. Rabbit antisera have been prepared which are specific for S, F and X.

Sheep red cells, `sensitized' with rabbit anti-sheep cell serum as for the Rose-Waaler test, were incubated with rheumatoid arthritis sera. Under appropriate conditions these cells failed to agglutinate when resuspended in saline, but could be agglutinated by anti-γ globulin (S+F), anti-S and anti-X, but not by anti-F. Cells incubated with normal serum failed to agglutinate. Thus rheumatoid arthritis sera contain a protein possessing the antigenic characteristics of β2M globulin which specifically coats sensitized sheep cells. This provides further evidence of the antibody nature of rheumatoid factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EDELMAN G. M., HEREMANS J. F., HEREMANS M. T., KUNKEL H. G. Immunological studies of human gamma-globulin. Relation of the precipitin lines of whole gamma-globulin to those of the fragments produced by papain. J Exp Med. 1960 Jul 1;112:203–223. doi: 10.1084/jem.112.1.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FRANKLIN E. C., HOLMAN H. R., MULLER-EBERHARD H. J., KUNKEL H. G. An unusual protein component of high molecular weight in the serum of certain patients with rheumatoid arthritis. J Exp Med. 1957 May 1;105(5):425–438. doi: 10.1084/jem.105.5.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FRANKLIN E. C., KUNKEL H. G. Immunologic differences between the 19 S and 7 S components of normal human gamma-globulin. J Immunol. 1957 Jan;78(1):11–18. [PubMed] [Google Scholar]
  4. FRANKLIN E. C., KUNKEL H. G., MULLER-EBERHARD H. J., HOLMAN H. R. Relation of high molecular weight proteins to the serological reactions in rheumatoid arthritis. Ann Rheum Dis. 1957 Sep;16(3):315–321. doi: 10.1136/ard.16.3.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRANKLIN E. C. Structural units of human 7S gamma globulin. J Clin Invest. 1960 Dec;39:1933–1941. doi: 10.1172/JCI104218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HELLER G., JACOBSON A. S., KOLODNY M. H., KAMMERER W. H. The hemagglutination test for rheumatoid arthritis. II. The influence of human plasma fraction II (gamma globulin) on the reaction. J Immunol. 1954 Jan;72(1):66–78. [PubMed] [Google Scholar]
  7. JAMES K., FELIX-DAVIES D., STANWORTH D. R. Studies on the isolation of rheumatoid factor. Ann Rheum Dis. 1961 Dec;20:369–385. doi: 10.1136/ard.20.4.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KUNKEL H. G., FRANKLIN E. C., MULLER-EBERHARD H. J. Studies on the isolation and characterization of the "rheumatoid factor". J Clin Invest. 1959 Feb;38(2):424–434. doi: 10.1172/JCI103817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MILGROM F., WITEBSKY E. Studies on the rheumatoid and related serum factors. I. Autoimmunization of rabbits with gamma globulin. JAMA. 1960 Sep 3;174:56–63. doi: 10.1001/jama.1960.63030010013013. [DOI] [PubMed] [Google Scholar]

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