Skip to main content
PMC home page
British Journal of Clinical Pharmacology logoLink to British Journal of Clinical Pharmacology
. 1976 Feb;3(1):135–143. doi: 10.1111/j.1365-2125.1976.tb00580.x

A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry.

B L Hillcoat, M Kawai, P B McCulloch, J Rosenfeld, C K Williams
PMCID: PMC1428807  PMID: 973935

Abstract

1 A gas chromatographic-mass spectrometric method was developed for determining 5-fluorouracil in plasma, using methylated thymine as an internal standard. 2 5-fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5-fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.

Full text

PDF
139

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brochmann-Hanssen E., Oke T. O. Gas chromatography of barbiturates, phenolic alkaloids, and xanthine bases: flash-heater methylation by means of trimethylanilinium hydroxide. J Pharm Sci. 1969 Mar;58(3):370–371. doi: 10.1002/jps.2600580322. [DOI] [PubMed] [Google Scholar]
  2. Cohen J. L., Brennan P. B. GLC assay for 5-fluorouracil in biological fluids. J Pharm Sci. 1973 Apr;62(4):572–575. doi: 10.1002/jps.2600620405. [DOI] [PubMed] [Google Scholar]
  3. Cohen J. L., Irwin L. E., Marshall G. J., Darvey H., Bateman J. R. Clinical pharmacology of oral and intravenous 5-fluorouracil (NSC-19893). Cancer Chemother Rep. 1974 Sep-Oct;58(5 Pt 1):723–731. [PubMed] [Google Scholar]
  4. Finj C., Sadée W. Determination of 5-fluorouracil (NSC-19893) plasma levels in rats and man by isotope dilution-mass fragmentography. Cancer Chemother Rep. 1975 Mar-Apr;59(2 Pt 1):279–286. [PubMed] [Google Scholar]
  5. HEIDELBERGER C., ANSFIELD F. J. EXPERIMENTAL AND CLINICAL USE OF FLUORINATED PYRIMIDINES IN CANCER CHEMOTHERAPY. Cancer Res. 1963 Sep;23:1226–1243. [PubMed] [Google Scholar]
  6. KING T. P., CRAIG L. C. Countercurrent distribution. Methods Biochem Anal. 1962;10:201–228. doi: 10.1002/9780470110270.ch7. [DOI] [PubMed] [Google Scholar]
  7. Pantarotto C., Martini A., Belvedere G., Bossi A., Donelli M. G., Frigerio A. Application of gas chromatography-chemical ionization mass fragmentography in the evaluation of bases and nucleoside analogues used in cancer chemotherapy. J Chromatogr. 1974 Nov 6;99(0):519–527. doi: 10.1016/s0021-9673(00)90881-1. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Clinical Pharmacology are provided here courtesy of British Pharmacological Society

RESOURCES