Abstract
1 Clinically normal human abdominal skin was irradiated with either three times its minimal erythema dose (MED) of ultraviolet B (u.v.B) or six MEDs of ultraviolet C (u.v.C) radiation. In both instances erythema was maximal at 24 h. 2 Exudate was recovered by a suction bulla technique from normal and irradiated skin at 24 h after irradiation. 3 Arachidonic acid, prostaglandins E2 and FSalpha, as measured by GC--MS, were significantly elevated at 24 h. Radioimmunoassay also showed increased PGF2alpha-like concentrations. 4 Oral indomethacin only partially reduced the erythema resulting from both types of radiation but totally suppressed the elevation of PGE 2 and F2alpha concentrations. 5 Topical indomethacin also suppressed u.v.B-induced increases in prostaglandins E2 and F2alpha. Unexpectedly, the vehicle alone produced a similar suppressive effect on prostaglandins although erythema appeared unaltered. 6 Most of the arachidonic acid metabolized by indomethacin-sensitive pathways is not converted to prostaglandins E2 and F2alpha in human skin.
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Selected References
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- Black A. K., Greaves M. W., Hensby C. N., Plummer N. A., Eady R. A. A new method for recovery of exudates from normal and inflamed human skin. Clin Exp Dermatol. 1977 Sep;2(3):209–216. doi: 10.1111/j.1365-2230.1977.tb02560.x. [DOI] [PubMed] [Google Scholar]
- Black A. K., Greaves M. W., Hensby C. N., Plummer N. A. Increased prostaglandins E2 and F2alpha in human skin at 6 and 24 h after ultraviolet B irradiation (290- 320 nm). Br J Clin Pharmacol. 1978 May;5(5):431–436. doi: 10.1111/j.1365-2125.1978.tb01650.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Camp R. D., Greaves M. W., Hensby C. N., Plummer N. A., Warin A. P. Irradiation of human skin by short wavelength ultraviolet radiation (100--290 nm) (u.v.C): increased concentrations of arachidonic acid and prostaglandines E2 and F2alpha. Br J Clin Pharmacol. 1978 Aug;6(2):145–148. doi: 10.1111/j.1365-2125.1978.tb00839.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dighe K. K., Emslie H. A., Henderson L. K., Simon L. The development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods. Br J Pharmacol. 1975 Dec;55(4):503–514. doi: 10.1111/j.1476-5381.1975.tb07425.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eaglstein W. H., Marsico A. R. Dichotomy in response to indomethacin in UV-C and UV-B induced ultraviolet light inflammation. J Invest Dermatol. 1975 Aug;65(2):238–240. doi: 10.1111/1523-1747.ep12598255. [DOI] [PubMed] [Google Scholar]
- Greenberg R. A., Eaglestein W. H., Turnier H., Houdek P. V. Orally given indomethacin and blood flow response to UVL. Arch Dermatol. 1975 Mar;111(3):328–330. [PubMed] [Google Scholar]
- Gruber C. M., Jr, Ridolfo A. S., Nickander R., Mikulaschek W. M. Delay of erythema of human skin by anti-inflammatory drugs after ultraviolet irradiation. Clin Pharmacol Ther. 1972 Jan-Feb;13(1):109–113. doi: 10.1002/cpt1972131109. [DOI] [PubMed] [Google Scholar]
- Miller W. S., Ruderman F. R., Smith J. G., Jr Aspirin and ultraviolet light-induced erythema in man. Arch Dermatol. 1967 Apr;95(4):357–358. [PubMed] [Google Scholar]
- Ramsay C. A., Challoner A. V. Vascular changes in human skin after ultraviolet irradiation. Br J Dermatol. 1976 May;94(5):487–493. doi: 10.1111/j.1365-2133.1976.tb05135.x. [DOI] [PubMed] [Google Scholar]
- Snyder D. S., Eaglstein W. H. Topical indomethacin and sunburn. Br J Dermatol. 1974 Jan;90(1):91–93. [PubMed] [Google Scholar]
- Sondergaard J., Greaves M. W. Pharmacological studies in inflammation due to exposure to ultraviolet radiation. J Pathol. 1970 Jun;101(2):93–97. doi: 10.1002/path.1711010204. [DOI] [PubMed] [Google Scholar]
- Thompson C. J., Los M., Horton E. W. The separation, identification and estimation of prostaglandins in nanogram quantities by combined gas chromatography-mass spectrometry. Life Sci I. 1970 Sep 1;9(17):983–988. doi: 10.1016/0024-3205(70)90121-9. [DOI] [PubMed] [Google Scholar]