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. 1988 Jun;29(6):809–815. doi: 10.1136/gut.29.6.809

Nitrogen losses from the human small bowel: obligatory losses and the effect of physical form of food.

A Chacko 1, J H Cummings 1
PMCID: PMC1433749  PMID: 2838402

Abstract

The amount and form of nitrogen lost from the human small intestine and the dietary factors which influence it have been studied in six ileostomists. Over a six day period the subjects were fed a series of diets including low nitrogen (LND) 0.17 g N/day, LND + soya beans (5.87 g N/day) and a high fibre diet (HFD) (10.6 g N/day). The soya beans were fed either whole or pureed to test the effect of physical form of food. Total N, protein, amino acids, urea, and ammonia were measured in ileostomy effluent which was collected throughout the study. Total N excretion was LND 0.91 (0.04) (SE) g/day; LND + whole soya beans (WSB) 2.26 (0.15) g/day; LND + pureed soya beans (PSB) 1.42 (0.12) g/day (WSB v PSB, p less than 0.001); and HFD 2.17 (0.11) g/day (HFD v PSB, p less than 0.001, HFD v WSB, NS). N losses as urea, ammonia, and free amino acids were less than 10-15% of total N, the remainder being protein (48-51%) and (by difference) peptides (20-30%). Eighty to 85% of effluent N was in the insoluble (pellet) fraction except on the low N diet where it was 66%. The physical form of food clearly influenced N digestibility in the soya beans whilst changes in dietary fibre seem not to have a significant effect.

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Selected References

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  1. Adibi S. A., Mercer D. W. Protein digestion in human intestine as reflected in luminal, mucosal, and plasma amino acid concentrations after meals. J Clin Invest. 1973 Jul;52(7):1586–1594. doi: 10.1172/JCI107335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bohe M., Borgström A., Genell S., Ohlsson K. Determination of immunoreactive trypsin, pancreatic elastase and chymotrypsin in extracts of human feces and ileostomy drainage. Digestion. 1983;27(1):8–15. doi: 10.1159/000198913. [DOI] [PubMed] [Google Scholar]
  3. Brock F. M., Forsberg C. W., Buchanan-Smith J. G. Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors. Appl Environ Microbiol. 1982 Sep;44(3):561–569. doi: 10.1128/aem.44.3.561-569.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cassidy M. M., Lightfoot F. G., Grau L. E., Story J. A., Kritchevsky D., Vahouny G. V. Effect of chronic intake of dietary fibers on the ultrastructural topography of rat jejunum and colon: a scanning electron microscopy study. Am J Clin Nutr. 1981 Feb;34(2):218–228. doi: 10.1093/ajcn/34.2.218. [DOI] [PubMed] [Google Scholar]
  5. Chapman R. W., Sillery J. K., Graham M. M., Saunders D. R. Absorption of starch by healthy ileostomates: effect of transit time and of carbohydrate load. Am J Clin Nutr. 1985 Jun;41(6):1244–1248. doi: 10.1093/ajcn/41.6.1244. [DOI] [PubMed] [Google Scholar]
  6. Cummings J. H. Fermentation in the human large intestine: evidence and implications for health. Lancet. 1983 May 28;1(8335):1206–1209. doi: 10.1016/s0140-6736(83)92478-9. [DOI] [PubMed] [Google Scholar]
  7. Cummings J. H., Hill M. J., Bone E. S., Branch W. J., Jenkins D. J. The effect of meat protein and dietary fiber on colonic function and metabolism. II. Bacterial metabolites in feces and urine. Am J Clin Nutr. 1979 Oct;32(10):2094–2101. doi: 10.1093/ajcn/32.10.2094. [DOI] [PubMed] [Google Scholar]
  8. Cummings J. H., Southgate D. A., Branch W. J., Wiggins H. S., Houston H., Jenkins D. J., Jivraj T., Hill M. J. The digestion of pectin in the human gut and its effect on calcium absorption and large bowel function. Br J Nutr. 1979 May;41(3):477–485. doi: 10.1079/bjn19790062. [DOI] [PubMed] [Google Scholar]
  9. DAHLQVIST A. A method for the determination of amylase in intestinal content. Scand J Clin Lab Invest. 1962;14:145–151. doi: 10.3109/00365516209079686. [DOI] [PubMed] [Google Scholar]
  10. Dunaif G., Schneeman B. O. The effect of dietary fiber on human pancreatic enzyme activity in vitro. Am J Clin Nutr. 1981 Jun;34(6):1034–1035. doi: 10.1093/ajcn/34.6.1034. [DOI] [PubMed] [Google Scholar]
  11. Englyst H. N., Cummings J. H. Digestion of the carbohydrates of banana (Musa paradisiaca sapientum) in the human small intestine. Am J Clin Nutr. 1986 Jul;44(1):42–50. doi: 10.1093/ajcn/44.1.42. [DOI] [PubMed] [Google Scholar]
  12. Finegold S. M., Sutter V. L., Boyle J. D., Shimada K. The normal flora of ileostomy and transverse colostomy effluents. J Infect Dis. 1970 Nov;122(5):376–381. doi: 10.1093/infdis/122.5.376. [DOI] [PubMed] [Google Scholar]
  13. Gibson J. A., Park N. J., Sladen G. E., Dawson A. M. The role of the colon in urea metabolism in man. Clin Sci Mol Med. 1976 Jan;50(1):51–59. doi: 10.1042/cs0500051. [DOI] [PubMed] [Google Scholar]
  14. Gibson J. A., Sladen G. E., Dawson A. M. Protein absorption and ammonia production: the effects of dietary protein and removal of the colon. Br J Nutr. 1976 Jan;35(1):61–65. doi: 10.1079/bjn19760009. [DOI] [PubMed] [Google Scholar]
  15. Goldberg D. M., McAllister R. A., Roy A. D. Studies on human intestinal proteolysis. Scand J Gastroenterol. 1968;3(2):193–201. doi: 10.3109/00365526809180122. [DOI] [PubMed] [Google Scholar]
  16. Gorbach S. L., Nahas L., Weinstein L., Levitan R., Patterson J. F. Studies of intestinal microflora. IV. The microflora of ileostomy effluent: a unique microbial ecology. Gastroenterology. 1967 Dec;53(6):874–880. [PubMed] [Google Scholar]
  17. Holgate A. M., Read N. W. Relationship between small bowel transit time and absorption of a solid meal. Influence of metoclopramide, magnesium sulfate, and lactulose. Dig Dis Sci. 1983 Sep;28(9):812–819. doi: 10.1007/BF01296904. [DOI] [PubMed] [Google Scholar]
  18. Kramer P. The effect of varying sodium loads on the ileal excreta of human ileostomized subjects. J Clin Invest. 1966 Nov;45(11):1710–1718. doi: 10.1172/JCI105477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Macfarlane G. T., Cummings J. H., Allison C. Protein degradation by human intestinal bacteria. J Gen Microbiol. 1986 Jun;132(6):1647–1656. doi: 10.1099/00221287-132-6-1647. [DOI] [PubMed] [Google Scholar]
  21. McNeil N. I., Bingham S., Cole T. J., Grant A. M., Cummings J. H. Diet and health of people with an ileostomy. 2. Ileostomy function and nutritional state. Br J Nutr. 1982 May;47(3):407–415. doi: 10.1079/bjn19820052. [DOI] [PubMed] [Google Scholar]
  22. NUGUID T. P., BACON H. E., BOUTWELL J., Jr An investigation of the volume of output and chemical content of ileal discharges following total colectomy and ileostomy. Surg Gynecol Obstet. 1961 Dec;113:733–742. [PubMed] [Google Scholar]
  23. Page M. A., Galton D. J. Glucose metabolites in blood and adipose tissue of obese diabetic and non-diabetic subjects. Clin Sci Mol Med. 1975 Jul;49(1):27–32. doi: 10.1042/cs0490027. [DOI] [PubMed] [Google Scholar]
  24. Roy A. D., Campbell R., Goldberg D. M. Effect of diet on the trypsin and chymotrypsin output in the stools of patients with an ileostomy. Gastroenterology. 1967 Oct;53(4):584–589. [PubMed] [Google Scholar]
  25. SMIDDY F. G., GREGORY S. D., SMITH I. B., GOLIGHER J. C. Faecal loss of fluid, electrolytes, and nitrogen in colitis before and after ileostomy. Lancet. 1960 Jan 2;1(7114):14–19. doi: 10.1016/s0140-6736(60)92717-3. [DOI] [PubMed] [Google Scholar]
  26. Sandberg A. S., Andersson H., Hallgren B., Hasselblad K., Isaksson B., Hultén L. Experimental model for in vivo determination of dietary fibre and its effect on the absorption of nutrients in the small intestine. Br J Nutr. 1981 Mar;45(2):283–294. doi: 10.1079/bjn19810105. [DOI] [PubMed] [Google Scholar]
  27. Snow P., O'Dea K. Factors affecting the rate of hydrolysis of starch in food. Am J Clin Nutr. 1981 Dec;34(12):2721–2727. doi: 10.1093/ajcn/34.12.2721. [DOI] [PubMed] [Google Scholar]
  28. Southgate D. A., Durnin J. V. Calorie conversion factors. An experimental reassessment of the factors used in the calculation of the energy value of human diets. Br J Nutr. 1970 Jun;24(2):517–535. doi: 10.1079/bjn19700050. [DOI] [PubMed] [Google Scholar]
  29. Wong S., O'Dea K. Importance of physical form rather than viscosity in determining the rate of starch hydrolysis in legumes. Am J Clin Nutr. 1983 Jan;37(1):66–70. doi: 10.1093/ajcn/37.1.66. [DOI] [PubMed] [Google Scholar]

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