Abstract
This investigation was aimed at comparing a new method for measuring faecal fat excretion, carried out with a semi-automated instrument by using near infrared analysis (NIRA), with the traditional titrimetric (Van de Kamer) and gravimetric (Sobel) methods. Near infrared analysis faecal fat was assayed on the three day stool collection from 118 patients (68 chronic pancreatitis, 19 organic diseases of the gastrointestinal tract, 19 alcoholic liver disease, 12 functional gastrointestinal disorders). A strict linear correlation was found between NIRA and both the titrimetric (r = 0.928, p less than 0.0001) and the gravimetric (r = 0.971, p less than 0.0001) methods. On homogenised faeces, a mean coefficient of variation of 2.1 (SD 1.71)% was found. Before homogenisation (where a mean coefficient of variation of 7% was found) accurate results were obtained when the mean of five measurements was considered. In conclusion, the assay of faecal fat excretion by the near infrared reflessometry appears a simple, rapid and reliable method for measuring steatorrhoea.
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Selected References
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- Fales F. W. Evaluation of a spectrophotometric method for determination of total fecal lipid. Clin Chem. 1971 Nov;17(11):1103–1108. [PubMed] [Google Scholar]
- Maschio C., Tomei E., Leonardis B., Cardinale E., Seri S. Valutazione della digestione e dell'assorbimento dei nutrienti nel sano ed in alcune condizioni di malassorbimento, mediante l'esame delle feci con un sistema automatizzato. G Clin Med. 1984 Feb;65(2):133–147. [PubMed] [Google Scholar]
- Newcomer A. D., Hofmann A. F., DiMagno E. P., Thomas P. J., Carlson G. L. Triolein breath test: a sensitive and specific test for fat malabsorption. Gastroenterology. 1979 Jan;76(1):6–13. [PubMed] [Google Scholar]
- Schneider M. U., Demling L., Jones S. A., Barker P. J., Domschke S., Heptner G., Domschke W. NMR spectrometry. A new method for total stool fat quantification in chronic pancreatitis. Dig Dis Sci. 1987 May;32(5):494–499. doi: 10.1007/BF01296032. [DOI] [PubMed] [Google Scholar]
- Thorsgaard Pedersen N., Nyboe Andersen B., Marqversen J. Estimation of 14C-triolein assimilation as a test of lipid assimilation. Breath test or measurement of serum radioactivity? Scand J Gastroenterol. 1982 Mar;17(2):309–316. doi: 10.3109/00365528209182059. [DOI] [PubMed] [Google Scholar]