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. 1984 Mar;45(3):503–507. doi: 10.1016/S0006-3495(84)84186-7

5-A Fourier map of gramicidin A phased by deuterium-hydrogen solvent difference neutron diffraction.

R E Koeppe 2nd, B P Schoenborn
PMCID: PMC1434879  PMID: 6201200

Abstract

Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The gramicidin dimer appears to be a 32-A-long cylinder oriented parallel to the c-axis in these crystals.

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Selected References

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  1. Bamberg E., Apell H. J., Alpes H. Structure of the gramicidin A channel: discrimination between the piL,D and the beta helix by electrical measurements with lipid bilayer membranes. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2402–2406. doi: 10.1073/pnas.74.6.2402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Crawford J. L., Kolpak F. J., Wang A. H., Quigley G. J., van Boom J. H., van der Marel G., Rich A. The tetramer d(CpGpCpG) crystallizes as a left-handed double helix. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4016–4020. doi: 10.1073/pnas.77.7.4016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Finkelstein A., Andersen O. S. The gramicidin A channel: a review of its permeability characteristics with special reference to the single-file aspect of transport. J Membr Biol. 1981 Apr 30;59(3):155–171. doi: 10.1007/BF01875422. [DOI] [PubMed] [Google Scholar]
  4. Koeppe R. E., 2nd, Berg J. M., Hodgson K. O., Stryer L. Gramicidin A crystals contain two cation binding sites per channel. Nature. 1979 Jun 21;279(5715):723–725. doi: 10.1038/279723a0. [DOI] [PubMed] [Google Scholar]
  5. Koeppe R. E., 2nd, Hodgson K. O., Stryer L. Helical channels in crystals of gramicidin A and of a cesium--gramicidin A complex: an x-ray diffraction study. J Mol Biol. 1978 May 5;121(1):41–54. doi: 10.1016/0022-2836(78)90261-9. [DOI] [PubMed] [Google Scholar]
  6. Sarkar N., Langley D., Paulus H. Biological function of gramicidin: selective inhibition of RNA polymerase. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1478–1482. doi: 10.1073/pnas.74.4.1478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schoenborn B. P. Neutron scattering for the analysis of membranes. Biochim Biophys Acta. 1976 Apr 13;457(1):41–55. doi: 10.1016/0304-4157(76)90013-7. [DOI] [PubMed] [Google Scholar]
  8. Urry D. W., Long M. M., Jacobs M., Harris R. D. Conformation and molecular mechanisms of carriers and channels. Ann N Y Acad Sci. 1975 Dec 30;264:203–220. doi: 10.1111/j.1749-6632.1975.tb31484.x. [DOI] [PubMed] [Google Scholar]
  9. Urry D. W., Prasad K. U., Trapane T. L. Location of monovalent cation binding sites in the gramicidin channel. Proc Natl Acad Sci U S A. 1982 Jan;79(2):390–394. doi: 10.1073/pnas.79.2.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Urry D. W. The gramicidin A transmembrane channel: a proposed pi(L,D) helix. Proc Natl Acad Sci U S A. 1971 Mar;68(3):672–676. doi: 10.1073/pnas.68.3.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wallace B. A., Veatch W. R., Blout E. R. Conformation of gramicidin A in phospholipid vesicles: circular dichroism studies of effects of ion binding, chemical modification, and lipid structure. Biochemistry. 1981 Sep 29;20(20):5754–5760. doi: 10.1021/bi00523a018. [DOI] [PubMed] [Google Scholar]
  12. Weinstein S., Wallace B. A., Morrow J. S., Veatch W. R. Conformation of the gramicidin A transmembrane channel: A 13C nuclear magnetic resonance study of 13C-enriched gramicidin in phosphatidylcholine vesicles. J Mol Biol. 1980 Oct 15;143(1):1–19. doi: 10.1016/0022-2836(80)90121-7. [DOI] [PubMed] [Google Scholar]

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