Abstract
The fluorescence quantum yield of a polymer molecule to which an energy donor chromophore and an energy acceptor chromophore are attached depends on the distance between the donor and acceptor chromophores. If this distance fluctuates with time, the fluorescence intensity is expected to fluctuate as well, and the time course of the intensity fluctuations will be correlated with the time course of the changes in the interchromophore distance. The intensity fluctuations are experimentally measurable if the number of illuminated molecules is small. A theoretical treatment of such fluorescence intensity fluctuations is presented in terms of a parameter that describes the polymer chain dynamics. Computer simulations were performed to illustrate the dependence of the autocorrelation function of the intensity fluctuations on the polymer chain conformation, the interchromophore energy transfer properties, and the macromolecular dynamics. These simulations demonstrate that the intensity fluctuations due to nonradiative energy transfer between chromophores attached to polymer chains can be large enough to be experimentally useful in the study of intramolecular dynamics of macromolecules.
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Selected References
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- Andries C., Guedens W., Clauwaert J., Geerts H. Photon and fluorescence correlation spectroscopy and light scattering of eye-lens proteins at moderate concentrations. Biophys J. 1983 Sep;43(3):345–354. doi: 10.1016/S0006-3495(83)84358-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehrenberg M., Rigler R. Fluorescence correlation spectroscopy applied to rotational diffusion of macromolecules. Q Rev Biophys. 1976 Feb;9(1):69–81. doi: 10.1017/s003358350000216x. [DOI] [PubMed] [Google Scholar]
- Elson E. L., Webb W. W. Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations. Annu Rev Biophys Bioeng. 1975;4(00):311–334. doi: 10.1146/annurev.bb.04.060175.001523. [DOI] [PubMed] [Google Scholar]
- Grinvald A., Haas E., Steinberg I. Z. Evaluation of the distribution of distances between energy donors and acceptors by fluorescence decay. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2273–2277. doi: 10.1073/pnas.69.8.2273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haas E., Katchalski-Katzir E., Steinberg I. Z. Effect of the orientation of donor and acceptor on the probability of energy transfer involving electronic transitions of mixed polarization. Biochemistry. 1978 Nov 14;17(23):5064–5070. doi: 10.1021/bi00616a032. [DOI] [PubMed] [Google Scholar]
- Haas E., Wilchek M., Katchalski-Katzir E., Steinberg I. Z. Distribution of end-to-end distances of oligopeptides in solution as estimated by energy transfer. Proc Natl Acad Sci U S A. 1975 May;72(5):1807–1811. doi: 10.1073/pnas.72.5.1807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Magde D., Elson E. L., Webb W. W. Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers. 1974 Jan;13(1):29–61. doi: 10.1002/bip.1974.360130103. [DOI] [PubMed] [Google Scholar]
- Sorscher S. M., Bartholomew J. C., Klein M. P. The use of fluorescence correlations spectroscopy to probe chromatin in the cell nucleus. Biochim Biophys Acta. 1980 Nov 14;610(1):28–46. doi: 10.1016/0005-2787(80)90053-2. [DOI] [PubMed] [Google Scholar]
- Stryer L., Haugland R. P. Energy transfer: a spectroscopic ruler. Proc Natl Acad Sci U S A. 1967 Aug;58(2):719–726. doi: 10.1073/pnas.58.2.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Webb W. W. Applications of fluorescence correlation spectroscopy. Q Rev Biophys. 1976 Feb;9(1):49–68. doi: 10.1017/s0033583500002158. [DOI] [PubMed] [Google Scholar]