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. 1985 Mar;47(3):381–385. doi: 10.1016/S0006-3495(85)83929-1

Bound trace element content of bovine retinal disk membranes as determined by particle-induced x-ray emission.

L D McCormick
PMCID: PMC1435226  PMID: 3978208

Abstract

Particle-induced x-ray emission (PIXE) was used to determine the trace element content of bovine retinal disk membranes. PIXE is a multielemental analytical technique capable of the simultaneous detection and quantization of all elements from sodium and above in atomic number. The multielemental capability of PIXE allows the analysis time per element to be very low if a number of elements are detected in each sample. In addition, the multielemental capability of PIXE can be used to determine elemental content with respect to an internal reference. Here the content of detected trace element per rhodopsin was determined without recourse to an external rhodopsin assay. This was accomplished by using the sulfur content of rhodopsin as an internal reference. Detected trace element contents per rhodopsin were 1.58 +/- 0.049 Ca, 0.081 +/- 0.024 Fe, 0.393 +/- 0.200 Cu, and 0.150 +/- 0.031 Zn. Upper limits were placed on the amount of manganese, molybdenum, and nickel per rhodopsin as 0.019, 0.019, and 0.006, respectively. Two proteins known to be present in disk membranes, retinol dehydrogenase and a large protein, approximately 238,000 mol wt, are considered as potential metallo-proteins. No correlation was observed between the content of any detected element and bleaching levels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaner W. S., Churchich J. E. The membrane bound retinol dehydrogenase from bovine rod outer segments. Biochem Biophys Res Commun. 1980 Jun 16;94(3):820–826. doi: 10.1016/0006-291x(80)91308-x. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Hagins W. A. The visual process: Excitatory mechanisms in the primary receptor cells. Annu Rev Biophys Bioeng. 1972;1:131–158. doi: 10.1146/annurev.bb.01.060172.001023. [DOI] [PubMed] [Google Scholar]
  4. Hargrave P. A., McDowell J. H., Curtis D. R., Wang J. K., Juszczak E., Fong S. L., Rao J. K., Argos P. The structure of bovine rhodopsin. Biophys Struct Mech. 1983;9(4):235–244. doi: 10.1007/BF00535659. [DOI] [PubMed] [Google Scholar]
  5. Hess H. H. The high calcium content of retinal pigmented epithelium. Exp Eye Res. 1975 Nov;21(5):471–479. doi: 10.1016/0014-4835(75)90128-1. [DOI] [PubMed] [Google Scholar]
  6. Liebman P. A. Rod disk calcium movement and transduction: a poorly illuminated story. Ann N Y Acad Sci. 1978 Apr 28;307:642–644. doi: 10.1111/j.1749-6632.1978.tb41987.x. [DOI] [PubMed] [Google Scholar]
  7. Papermaster D. S., Converse C. A., Zorn M. Biosynthetic and immunochemical characterization of large protein in frog and cattle rod outer segment membranes. Exp Eye Res. 1976 Aug;23(2):105–115. doi: 10.1016/0014-4835(76)90194-9. [DOI] [PubMed] [Google Scholar]
  8. Pober J. S., Bitensky M. W. Light-regulated enzymes of vertebrate retinal rods. Adv Cyclic Nucleotide Res. 1979;11:265–301. [PubMed] [Google Scholar]
  9. Pourcho R. G. Distribution of [35S]taurine in mouse retina after intravitreal and intravascular injection. Exp Eye Res. 1977 Aug;25(2):119–127. doi: 10.1016/0014-4835(77)90124-5. [DOI] [PubMed] [Google Scholar]
  10. Schnetkamp P. P. Calcium translocation and storage of isolated intact cattle rod outer segments in darkness. Biochim Biophys Acta. 1979 Jul 5;554(2):441–459. doi: 10.1016/0005-2736(79)90383-3. [DOI] [PubMed] [Google Scholar]
  11. Smith H. G., Jr, Stubbs G. W., Litman B. J. The isolation and purification of osmotically intact discs from retinal rod outer segments. Exp Eye Res. 1975 Mar;20(3):211–217. doi: 10.1016/0014-4835(75)90134-7. [DOI] [PubMed] [Google Scholar]
  12. Szuts E. Z., Cone R. A. Calcium content of frog rod outer segments and discs. Biochim Biophys Acta. 1977 Jul 14;468(2):194–208. doi: 10.1016/0005-2736(77)90114-6. [DOI] [PubMed] [Google Scholar]
  13. Tam S. W., Wilber K. E., Wagner F. W. Light sensitive zinc content of protein fractions from bovine rod outer segments. Biochem Biophys Res Commun. 1976 Sep 7;72(1):302–309. doi: 10.1016/0006-291x(76)90994-3. [DOI] [PubMed] [Google Scholar]

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