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. 1976 Apr;30(4):559–564.

Role of membrane structures in B-lymphocyte activation. The effect of binding anti-immunoglobulin, aggregated IgG and immune complexes.

R Ramasamy
PMCID: PMC1445019  PMID: 1083830

Abstract

It is shown that the binding of bivalent anti-immunoglobulin preparations to the surface immunoglobulin of murine B lymphocytes as well as the binding of aggregates of IgG or immune complexes does not lead to detectable increase in cell division in microcultures. Treating lymphocytes with immune complexes and aggregated IgG does not abolish the subsequent mitogenic response induced by LPS. The background mitosis observed in culture is inhibited with anti-immunoglobulin antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Bullock W. W., Melchers F. Inhibition of mitogenic stimulation of mouse lymphocytes by anti-mouse immunoglobulin antibodies. I. Mode of action. Eur J Immunol. 1974 Nov;4(11):715–722. doi: 10.1002/eji.1830041103. [DOI] [PubMed] [Google Scholar]
  2. Basten A., Miller J. F., Abraham R. Relationship between Fc receptors, antigen-binding sites on T and B cells, and H-2 complex-associated determinants. J Exp Med. 1975 Mar 1;141(3):547–560. doi: 10.1084/jem.141.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coutinho A. The theory of the 'one nonspecific signal' model for b cell activation. Transplant Rev. 1975;23:49–65. doi: 10.1111/j.1600-065x.1975.tb00148.x. [DOI] [PubMed] [Google Scholar]
  4. Dickler H. B., Sachs D. H. Evidence for identity or close association of the Fc receptor of B lymphocytes and alloantigens determined by the Ir region of the H-2 complex. J Exp Med. 1974 Sep 1;140(3):779–796. doi: 10.1084/jem.140.3.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elson C. J., Singh J., Taylor R. B. The effect of capping by anti-immunoglobulin antibody on the expression of cell surface immunoglobulin and on lymphocyte activation. Scand J Immunol. 1973;2(2):143–149. doi: 10.1111/j.1365-3083.1973.tb02025.x. [DOI] [PubMed] [Google Scholar]
  6. Janossy G., Greaves M. Functional analysis of murine and human B lymphocyte subsets. Transplant Rev. 1975;24:177–236. doi: 10.1111/j.1600-065x.1975.tb00169.x. [DOI] [PubMed] [Google Scholar]
  7. Kishimoto T., Ishizaka K. Regulation of antibody response in vitro. IX. Induction of secondary anti-hapten IgG antibody response by anti-immunoglobulin and enhancing soluble factor. J Immunol. 1975 Feb;114(2 Pt 1):585–591. [PubMed] [Google Scholar]
  8. Marchalonis J. J., Cone R. E. Biochemical and biological characteristics of lymphocyte surface immunoglobulin. Transplant Rev. 1973;14:3–49. doi: 10.1111/j.1600-065x.1973.tb00101.x. [DOI] [PubMed] [Google Scholar]
  9. Munro A. J., Taussig M. J. Two genes in the major histocompatibility complex control immune response. Nature. 1975 Jul 10;256(5513):103–106. doi: 10.1038/256103a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Möller G., Coutinho A. Role of C'3 and Fc receptors in B-lymphocyte activation. J Exp Med. 1975 Mar 1;141(3):647–663. doi: 10.1084/jem.141.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Möller G. Induction of DNA synthesis in normal human lymphocyte cultures by antigen-antibody complexes. Clin Exp Immunol. 1969 Jan;4(1):65–82. [PMC free article] [PubMed] [Google Scholar]
  12. Ramasamy R., Munro A., Milstein C. Possible role for the Fc receptor on B lymphocytes. Nature. 1974 Jun 7;249(457):573–574. doi: 10.1038/249573a0. [DOI] [PubMed] [Google Scholar]
  13. Sell S. Development of restrictions in the expression of immunoglobulin specificities by lymphoid cells. Transplant Rev. 1970;5:19–44. doi: 10.1111/j.1600-065x.1970.tb00355.x. [DOI] [PubMed] [Google Scholar]
  14. Sell S., Rowe D. S., Gell P. G. Studies on rabbit lymphocytes in vitro. 3. Proteins, RNA, and DNA synthesis by lymphocyte cultures after stimulation with phytohaemagglutinin, with staphylococcal filtrate, with antiallotype serum, and with heterologous antiserum to rabbit whole serum. J Exp Med. 1965 Oct 1;122(4):823–839. doi: 10.1084/jem.122.4.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vitetta E. S., Uhr J. W. Synthesis, transport, dynamics and fate of cell surface Ig and alloantigens in murine lymphocytes. Transplant Rev. 1973;14:50–75. doi: 10.1111/j.1600-065x.1973.tb00102.x. [DOI] [PubMed] [Google Scholar]
  16. Weber W. T. Avian B lymphocyte subpopulations: origins and functional capacities. Transplant Rev. 1975;24:113–158. doi: 10.1111/j.1600-065x.1975.tb00167.x. [DOI] [PubMed] [Google Scholar]

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