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. 1976 Jun;30(6):935–949.

A lipid chemotactic factor from anaerobic coryneform bacteria including Corynebacterium parvum with activity for macrophages and monocytes.

R J Russell, R J McInroy, P C Wilkinson, R G White
PMCID: PMC1445065  PMID: 1027716

Abstract

A lipid with chemoattractant (chemotactic) activity for mouse and guinea-pig macrophages and for human blood monocytes is released by anaerobic coryneform bacteria (including Corynebacterium parvum). The active lipid is associated with fibrillar structures which lie on the outside of the bacterial cell and are released spontaneously during growth. The lipid can also be extracted easily by a number of methods. The fibrils are loosely associated with a capsule-like structure composed largely of polysaccharide. Purification of the active lipid was achieved by chloroform-methanol extraction of the whole organisms yielding a chloroform-soluble fraction attracting mononuclear phagocytes at concentrations around 10 microgram/ml. The infra-red spectrum of this material showed lipid but no peptide or sugar. Thin-layer chromatography yielded twelve spots of which three had chemoattractant properties. The most active of these gave staining reactions consistent with the presence of phospholipid, the other two probably contained free fatty acids and triglycerides. Thin-layer electrophoresis also yielded an active phosphorus-containing spot. Saturated fatty acids of chain lengths found in the anaerobic coryne forms had weak monocyte-attractant activity. As the active material was progressively purified, its activity as a monocyte attractant weakened.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. ETEMADI A. H. ISOLEMENT DES ACIDES ISOPENTAD'ECANOUIQUE ET ISOHEPTAD'ECANOUIQUE DES LIPIDES DE CORYNEBACTERIUM PARVUM. Bull Soc Chim Biol (Paris) 1963;45:1423–1432. [PubMed] [Google Scholar]
  3. Fauve R. M., Hevin B. Immunostimulation with bacterial phospholipid extracts. Proc Natl Acad Sci U S A. 1974 Feb;71(2):573–577. doi: 10.1073/pnas.71.2.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gordon J., White R. G. Surface peptido-glycolipid filaments on Mycobacterium leprae. Clin Exp Immunol. 1971 Oct;9(4):539–547. [PMC free article] [PubMed] [Google Scholar]
  5. Johnson J. L., Cummins C. S. Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica. J Bacteriol. 1972 Mar;109(3):1047–1066. doi: 10.1128/jb.109.3.1047-1066.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. NEVEU T., BRANELLEC A., BIOZZI G. PROPRI'ET'ES ADJUVANTES DE CORYNEBACTERIUM PARVUM SUR LA PRODUCTION D'ANTICORPS ET SUR L'INDUCTION DE L'HYPERSENSIBILIT'E RETARD'EE ENVERS LES PROT'EINES CONJUGU'EES. Ann Inst Pasteur (Paris) 1964 May;106:771–777. [PubMed] [Google Scholar]
  7. O'Neill G. J., Henderson D. C., White R. G. The role of anaerobic coryneforms on specific and non-specific immunological reactions. I. Effect on particle clearance and humoral and cell-mediated immunological responses. Immunology. 1973 Jun;24(6):977–995. [PMC free article] [PubMed] [Google Scholar]
  8. Turner S. R., Campbell J. A., Lynn W. S. Polymorphonulcear leukocyte chemotaxis toward oxidized lipid components of cell membranes. J Exp Med. 1975 Jun 1;141(6):1437–1441. doi: 10.1084/jem.141.6.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Vaskovsky V. E., Kostetsky E. Y. Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res. 1968 May;9(3):396–396. [PubMed] [Google Scholar]
  10. Wilkinson P. C., O'Neill G. J., Wapshaw K. G. Role of anaerobic coryneforms in specific and non-specific immunological reactions. II. Production of a chemotactic factor specific for macrophages. Immunology. 1973 Jun;24(6):997–1006. [PMC free article] [PubMed] [Google Scholar]
  11. Woodruff M. F., Boak J. L. Inhibitory effect of injection of Corynebacterium parvum on the growth of tumour transplants in isogenic hosts. Br J Cancer. 1966 Jun;20(2):345–355. doi: 10.1038/bjc.1966.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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