Skip to main content
Immunology logoLink to Immunology
. 1976 Aug;31(2):249–255.

Helper T-cell kinetics and investigation of antigen receptor expression on early and memory T-helper cells.

R Mohr, U Krawinkel
PMCID: PMC1445114  PMID: 1085284

Abstract

The subcutaneous application of soluble antigen resulted in generation of helper T lymphocytes in the draining lymph nodes, tested 4 days after priming in the adoptive transfer system. Four days later a specific helper T-cell population was detected in the spleen. B-cell activity could be demonstrated 4-5 days after helper T-cell activity in both organs. We investigated the early helper T-cell population and memory T cells with respect to differences in the expression of antigen receptors. No such differences could be detected, either by anti-immunoglobulin-coated Degalan columns or by an antigen-specific rosetting method.

Full text

PDF
249

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashman R. F., Raff M. C. Direct demonstration of theta-positive antigen-binding cells, with antigen-induced movement of thymus-dependent cell receptors. J Exp Med. 1973 Jan 1;137(1):69–84. doi: 10.1084/jem.137.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach J. F., Dardenne M. Antigen recognition by T lymphocytes. Thymus and marrow dependence of spontaneous rosette forming cells in the mouse. Cell Immunol. 1972 Jan;3(1):1–10. doi: 10.1016/0008-8749(72)90220-1. [DOI] [PubMed] [Google Scholar]
  3. Basten A., Miller J. F., Warner N. L., Pye J. Specific inactivation of thymus-derived (T) and non-thymus-derived (B) lymphocytes by 125I-labelled antigen. Nat New Biol. 1971 May 26;231(21):104–106. doi: 10.1038/newbio231104a0. [DOI] [PubMed] [Google Scholar]
  4. Black S. J., Inchley C. J. Characteristics of immunological memory in mice. I. Separate early generation of cells mediating IgM and IgG memory to sheep erythrocytes. J Exp Med. 1974 Aug 1;140(2):333–348. doi: 10.1084/jem.140.2.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cunningham A. J., Sercarz E. E. The asynchronous development of immunological memory in helper (T) and precursor (B) cell lines. Eur J Immunol. 1971 Dec;1(6):413–421. doi: 10.1002/eji.1830010602. [DOI] [PubMed] [Google Scholar]
  6. Elliott B. E., Haskill J. S., Axelrad M. A. Thymus-derived rosettes are not "helper" cells. J Exp Med. 1973 Nov 1;138(5):1133–1143. doi: 10.1084/jem.138.5.1133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elliott B. E., Haskill J. S. Rosette-forming ability of thymus-derived lymphocytes in humoral and cell-mediated immunity. II. Helper cell activity. J Exp Med. 1975 Mar 1;141(3):600–607. doi: 10.1084/jem.141.3.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JANDL J. H., SIMMONS R. L. The agglutination and sensitization of red cells by metallic cations: interactions between multivalent metals and the red-cell membrane. Br J Haematol. 1957 Jan;3(1):19–38. doi: 10.1111/j.1365-2141.1957.tb05768.x. [DOI] [PubMed] [Google Scholar]
  9. Kappler J. W., Hoffmann M. Regulation of the immune response. 3. Kinetic differences between thymus- and bone marrow- derived lymphocytes in the proliferative response to heterologous erythrocytes. J Exp Med. 1973 Jun 1;137(6):1325–1337. doi: 10.1084/jem.137.6.1325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kettman J., Dutton R. W. Radioresistance of the enhancing effect of cells from carrier-immunized mice in an in vitro primary immune response. Proc Natl Acad Sci U S A. 1971 Apr;68(4):699–703. doi: 10.1073/pnas.68.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McConnell I., Munro A., Gurner B. W., Coombs R. R. Studies on actively allergized cells. I. The cyto-dynamics and morphology of rosete-forming lymph node cells in mice and inhibition of rosette-formation with antibody to mouse immunoglobulins. Int Arch Allergy Appl Immunol. 1969;35(3):209–227. [PubMed] [Google Scholar]
  12. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. I. Measurement of the effect with transferred cells and objections to the local environment hypothesis. Eur J Immunol. 1971 Jan;1(1):10–17. doi: 10.1002/eji.1830010103. [DOI] [PubMed] [Google Scholar]
  13. NOSSAL G. J., ADA G. L., AUSTIN C. M. ANTIGENS IN IMMUNITY. II. IMMUNOGENIC PROPERTIES OF FLAGELLA, POLYMERIZED FLAGELLIN AND FLAGELLIN IN THE PRIMARY RESPONSE. Aust J Exp Biol Med Sci. 1964 Jun;42:283–294. [PubMed] [Google Scholar]
  14. Rajewsky K., Brenig C. Paralysis to serum albumins in T and B lymphocytes in mice. Dose dependence, specificity and kinetics of escape. Eur J Immunol. 1974 Feb;4(2):120–125. doi: 10.1002/eji.1830040211. [DOI] [PubMed] [Google Scholar]
  15. Rajewsky K., Mohr R. Specificity and heterogeneity of helper T cells in the response to serum albumins in mice. Eur J Immunol. 1974 Feb;4(2):111–119. doi: 10.1002/eji.1830040210. [DOI] [PubMed] [Google Scholar]
  16. Roelants G. E., Askonas B. A. Cell cooperation in antibody induction. The susceptibility of helper cells to specific lethal radioactive antigen. Eur J Immunol. 1971 Jun;1(3):151–157. doi: 10.1002/eji.1830010302. [DOI] [PubMed] [Google Scholar]
  17. Roelants G. E., Rydén A., Hägg L. B., Loor F. Active synthesis of immunoglobulin receptors for antigen by T lymphocytes. Nature. 1974 Jan 11;247(5436):106–108. doi: 10.1038/247106a0. [DOI] [PubMed] [Google Scholar]
  18. Roelants G., Rydén A. Dose dependence of antigen binding to B and T lymphocytes. Nature. 1974 Jan 11;247(5436):104–106. doi: 10.1038/247104a0. [DOI] [PubMed] [Google Scholar]
  19. Sprent J., Miller J. F., Mitchell G. F. Antigen-induced selective recruitment of circulating lymphocytes. Cell Immunol. 1971 Apr;2(2):171–181. doi: 10.1016/0008-8749(71)90036-0. [DOI] [PubMed] [Google Scholar]
  20. Wigzell H., Sundqvist K. G., Yoshida T. O. Separation of cells according to surface antigens by the use of antibody-coated columns. Fractionation of cells carrying immunoglobulins and blood group antigen. Scand J Immunol. 1972;1(1):75–87. doi: 10.1111/j.1365-3083.1972.tb03737.x. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES