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. 1976 Mar;30(3):419–424.

Antibody to Physarum myosin. I. Preparation and functional effects.

V T Nachmias, D Kessler
PMCID: PMC1445155  PMID: 130343

Abstract

Preparation of antibody to Physarum myosin is described, and evidence is presented that the antibody is specific for this molecule. A diffusion coefficient of 1 X 10(-7) cm2/s is estimated. The antibody interfered with myosin enzyme activity and with superprecipitation of actomyosin. It did not cross-react with rabbit striated muscle myosin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adelman M. R., Taylor E. W. Further purification and characterization of slime mold myosin and slime mold actin. Biochemistry. 1969 Dec;8(12):4976–4988. doi: 10.1021/bi00840a047. [DOI] [PubMed] [Google Scholar]
  2. DANIEL J. W., RUSCH H. P. The pure culture of Physarum polycephalum on a partially defined soluble medium. J Gen Microbiol. 1961 May;25:47–59. doi: 10.1099/00221287-25-1-47. [DOI] [PubMed] [Google Scholar]
  3. FINGER I., HELLER C. Immunogenetic analysis of proteins of Paramecium. I. Comparison of specificities controlled by alleles and by different loci. Genetics. 1962 Feb;47:223–239. doi: 10.1093/genetics/47.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hatano S., Tazawa M. Isolation, purification and characterization of byosin B from myxomycete plasmodium. Biochim Biophys Acta. 1968 Apr 9;154(3):507–519. doi: 10.1016/0005-2795(68)90011-1. [DOI] [PubMed] [Google Scholar]
  5. Nachmias V. T. Properties of Physarum myosin purified by a potassium iodide procedure. J Cell Biol. 1974 Jul;62(1):54–65. doi: 10.1083/jcb.62.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nachmias V. Filament formation by purified Physarum myosin. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2011–2014. doi: 10.1073/pnas.69.8.2011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PREER J. R., Jr A quantitative study of a technique of double diffusion in agar. J Immunol. 1956 Jul;77(1):52–60. [PubMed] [Google Scholar]
  8. Pepe F. A. Some aspects of the structural organization of the myofibril as revealed by antibody--staining methods. J Cell Biol. 1966 Mar;28(3):505–525. doi: 10.1083/jcb.28.3.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pepe F. A. The myosin filament. II. Interaction between myosin and actin filaments observed using antibody staining in fluorescent and electron microscopy. J Mol Biol. 1967 Jul 28;27(2):227–236. doi: 10.1016/0022-2836(67)90017-4. [DOI] [PubMed] [Google Scholar]

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