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. 1976 Dec;31(6):913–919.

Kinetics and localization of IgE tetanus antibody response in mice immunized by the intratracheal, intraperitoneal and subcutaneous routes.

J L Gerbrandy, J Bienenstock
PMCID: PMC1445178  PMID: 992717

Abstract

The heterologous adoptive cutaneous anaphylaxis system was used to determine the kinetics of appearance of IgE-producing cells in various lymphoid tissues of mice following intratracheal (i.t.), intraperitoneal (i.p.), or subcutaneous (s.c.) immunization with tetanus toxoid and Bordetella pertussis organisms. Immunization, i.t. and i.p., produced similar patterns of response with the bronchial lymph nodes quantitatively exceeding the responses in other lymphoid tissues. In both cases the splenic lymphocyte response was second only to the bronchial and both appeared to parallel the serum PCA antibody. It is suggested that both responses represent draining lymph node responses since the bronchial lymph node drains both sites of immunization. After s.c. immunization a primary response of low order was found in the draining popliteal lymph node but not elsewhere. Although a dissociation was seen between responses obtained in various lymphoid tissues following s.c. and i.p. or i.t. immunization, no real evidence for a local mucosal response, such as has been reported for IgA, was obtained. These results lend experimental support to the observations that intratracheal and intraperitoneal immunization routes are most effective in production of IgE antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazin H., Malet F. The metabolism of different immunoglobulin classes in irradiated mice. Immunology. 1969 Sep;17(3):345–365. [PMC free article] [PubMed] [Google Scholar]
  2. Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Deuschl H., Johansson S. G. Immunoglobulins in tracheo-bronchial secretion with special reference to IgE. Clin Exp Immunol. 1974 Mar;16(3):401–412. [PMC free article] [PubMed] [Google Scholar]
  4. Dolezel J., Bienenstock J. A and non- A immune response after oral and parenteral immunization of the hamster. Cell Immunol. 1971 Oct;2(5):458–468. doi: 10.1016/0008-8749(71)90056-6. [DOI] [PubMed] [Google Scholar]
  5. Gerbrandy J. L., van Dura E. A. Anamnestic secretory antibody response in respiratory secretions of intranasally immunized mice. J Immunol. 1972 Nov;109(5):1146–1148. [PubMed] [Google Scholar]
  6. Ishizaka K., Newcomb R. W. Presence of gammaE in nasal washings and sputum from asthmatic patients. J Allergy. 1970 Oct;46(4):197–204. doi: 10.1016/0021-8707(70)90023-7. [DOI] [PubMed] [Google Scholar]
  7. Kaltreider H. B., Kyselka L., Salmon S. E. Immunology of the lower respiratory tract. II. The plaque-forming response of canine lymphoid tissues to sheep erythrocytes after intrapulmonary or intravenous immunization. J Clin Invest. 1974 Aug;54(2):263–270. doi: 10.1172/JCI107761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kind L. S., Macedo-Sobrinho B. Communications. Heterologous adoptive cutaneous anaphylaxis: a method for detecting reaginic antibody formation by cells of the mouse. J Immunol. 1973 Aug;111(2):638–640. [PubMed] [Google Scholar]
  9. Kind L. S., Malloy W. F. Development of reaginic antibody-forming cells in the spleen and bone marrow of immunized mice. J Immunol. 1974 Apr;112(4):1609–1612. [PubMed] [Google Scholar]
  10. Lee W. Y., Sehon A. H. Suppression of reaginic antibody formation. II. The use of adoptive transfer system for the study of immunological unresponsiveness. J Immunol. 1975 Feb;114(2 Pt 2):837–842. [PubMed] [Google Scholar]
  11. Mota I., Wong D. Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. Life Sci. 1969 Aug 15;8(16):813–820. doi: 10.1016/0024-3205(69)90099-x. [DOI] [PubMed] [Google Scholar]
  12. Nash D. R. Direct and indirect plaque forming cells in extrapulmonary lymphoid tissue following local vs systemic injection of soluble antigen. Cell Immunol. 1973 Nov;9(2):234–241. doi: 10.1016/0008-8749(73)90074-9. [DOI] [PubMed] [Google Scholar]
  13. Nash D. R., Holle B. Local and systemic cellular immune responses in guinea-pigs given antigen parenterally or directly into the lower respiratory tract. Clin Exp Immunol. 1973 Apr;13(4):573–583. [PMC free article] [PubMed] [Google Scholar]
  14. Okudaira H., Ishizaka K. Reaginic antibody formation in the mouse. IV. Adoptive anti-hapten IgE antibody response in irradiated recipients of hapten-primed cells and carrier-specific cells. J Immunol. 1974 Aug;113(2):563–573. [PubMed] [Google Scholar]
  15. Okumura K., Tada T. Regulation of homocytotropic antibody formation in the rat. IX. Further characterization of the antigen-specific inhibitory T cell factor in hapten-specific homocytotropic antibody response. J Immunol. 1974 Feb;112(2):783–791. [PubMed] [Google Scholar]
  16. Rudzik R., Clancy R. L., Perey D. Y., Day R. P., Bienenstock J. Repopulation with IgA-containing cells of bronchial and intestinal lamina propria after transfer of homologous Peyer's patch and bronchial lymphocytes. J Immunol. 1975 May;114(5):1599–1604. [PubMed] [Google Scholar]
  17. Tada T., Ishizaka K. Distribution of gamma E-forming cells in lymphoid tissues of the human and monkey. J Immunol. 1970 Feb;104(2):377–387. [PubMed] [Google Scholar]
  18. Tada T., Okumura K. Regulation of homocytotropic antibody formation in the rat. I. Feed-back regulation by passively administered antibody. J Immunol. 1971 Apr;106(4):1002–1011. [PubMed] [Google Scholar]
  19. Van Hout C. A., Johnson H. G. Synthesis of rat IgE by aerosol immunization. J Immunol. 1972 Mar;108(3):834–836. [PubMed] [Google Scholar]
  20. Waldman R. H., Henney C. S. Cell-mediated immunity and antibody responses in the respiratory tract after local and systemic immunization. J Exp Med. 1971 Aug 1;134(2):482–494. doi: 10.1084/jem.134.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Waldman R. H., Virchow C., Rowe D. S. IgE levels in external secretions. Int Arch Allergy Appl Immunol. 1973;44(2):242–248. doi: 10.1159/000230933. [DOI] [PubMed] [Google Scholar]
  22. Waldmann T. A. Disorders of immunoglobulin metabolism. N Engl J Med. 1969 Nov 20;281(21):1170–1177. doi: 10.1056/NEJM196911202812107. [DOI] [PubMed] [Google Scholar]
  23. Werner H. J., Lefèvre H., Raettig H. Biphasic occurrence of IgM-forming cells in the intestine after oral immunization. Nature. 1971 Jul 9;232(5306):119–120. doi: 10.1038/232119a0. [DOI] [PubMed] [Google Scholar]

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