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. 1976 Sep;31(3):417–424.

Immune response in the garter snake (Thamnophis ordinoides).

J E Coe, D Leong, J L Portis, L A Thomas
PMCID: PMC1445261  PMID: 1027724

Abstract

Garter snakes (Thamnophis ordinoides) were immunized with hen egg albumin, human gamma-globulin and Keyhole limpet haemocyanin in Freund's adjuvant. Antibody was consistently detected by radioimmunoelectrophoresis and in three different gamma- and beta-globulin precipitin lines called IgM (approximately or equal to 20S), Ig-1 (approximately or equal to 9S) and Ig-2 (approximately or equal to 8-5S). Early antibody (day 31 after immunization) was frequently Ig-M whereas Ig-2 and especially Ig-1 were detectable for the longest duration (992 days). After immunization with antigen in Freund's adjuvant, Ig-1 serum concentration showed the greatest increase, from almost undetectable levels to the most prominent immunoglobulin in immune serum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acton R. T., Evans E. E., Weinheimer P. F., Niedermeier W., Bennett J. C. Purification and characterization of two classes of immunoglobulins from the marine toad, Bufo marinus. Biochemistry. 1972 Jul 18;11(15):2751–2759. doi: 10.1021/bi00765a003. [DOI] [PubMed] [Google Scholar]
  2. Allison A. C., Davies A. J. Requirement of thymus-dependent lymphocytes for potentiation by adjuvants of antibody formation. Nature. 1971 Oct 1;233(5318):330–332. doi: 10.1038/233330a0. [DOI] [PubMed] [Google Scholar]
  3. Coe J. E., Peel L. F. Antibody production in the bullfrog (Rana catesbeiana). Immunology. 1970 Oct;19(4):539–550. [PMC free article] [PubMed] [Google Scholar]
  4. Coe J. E. The immune response in the hamster. I. Definition of two 7 S globulin classes: 7 S-gamma-1 and 7 S-gamma-2. J Immunol. 1968 Mar;100(3):507–515. [PubMed] [Google Scholar]
  5. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  6. Du Pasquier L., Weiss N., Loor F. Direct evidence for immunoglobulins on the surface of thymus lymphocytes of amphibian larvae. Eur J Immunol. 1972 Aug;2(4):366–370. doi: 10.1002/eji.1830020414. [DOI] [PubMed] [Google Scholar]
  7. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  8. Linna T. J., Finstad J., Fichtelius K. E., Good R. A. Proliferative responses of cyclostomes to stimulation with antigen in adjuvant. Acta Pathol Microbiol Scand A. 1970;78(2):169–178. doi: 10.1111/j.1699-0463.1970.tb00251.x. [DOI] [PubMed] [Google Scholar]
  9. Lykakis J. J., Cox F. E. Immunological responses of the toad, Xenopus laevis, to the antigens of the ciliate, Tetrahymena pyriformis. Immunology. 1968 Sep;15(3):429–437. [PMC free article] [PubMed] [Google Scholar]
  10. Marchalonis J. J., Edelman G. M. Phylogenetic origins of antibody structure. 3. Antibodies in the primary immune response of the sea lamprey, Petromyzon marinus. J Exp Med. 1968 May 1;127(5):891–914. doi: 10.1084/jem.127.5.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marchalonis J., Edelman G. M. Phylogenetic origins of antibody structure. II. Immunoglobulins in the primary immune response of the bullfrog, Rana catesbiana. J Exp Med. 1966 Nov 1;124(5):901–913. doi: 10.1084/jem.124.5.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  13. Portis J. L., Coe J. E. IgM the secretory immunoglobulin of reptiles and amphibians. Nature. 1975 Dec 11;258(5535):547–548. doi: 10.1038/258547a0. [DOI] [PubMed] [Google Scholar]
  14. THOMAS L. A., EKLUND C. M. Overwintering of western equine encephalomyelitis virus in garter snakes experimentally infected by Culex tarsalis. Proc Soc Exp Biol Med. 1962 Feb;109:421–424. doi: 10.3181/00379727-109-27225. [DOI] [PubMed] [Google Scholar]
  15. Tam L. Q., Benedict A. A. Elevated 7S immunoglobulin and acute phase proteins in adjuvant-injected chickens. Proc Soc Exp Biol Med. 1975 Nov;150(2):340–346. doi: 10.3181/00379727-150-39032. [DOI] [PubMed] [Google Scholar]
  16. WEIGLE W. O. IMMUNOCHEMICAL PROPERTIES OF HEMOCYANIN. Immunochemistry. 1964 Dec;1:295–302. doi: 10.1016/0019-2791(64)90030-8. [DOI] [PubMed] [Google Scholar]

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