Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1977 Mar;32(3):237–245.

Cell-mediated and humoral immune responses in mice. IV. Difference of the functional cell population between helper activity and delayed-type hypersensitivity.

Y Katsura, M Takaoki, S Nishikawa, S Muramatsu
PMCID: PMC1445285  PMID: 321345

Abstract

Helper activity in the anti-hapten antibody response was studied in mice in reference to the induction of delayed-type hypersensitivity (DTH) to the carrier protein. Mice were immunized either by an i.v. injection of alum-precipitated bovine serum albumin (AP-BSA) plus bacterial endotoxin or by a s.c. injection of BSA in Freund's complete adjuvant, the latter being effective in inducing DTH. The helper activity was estimated by the antibody response to the challenge with dinitrophenylated BSA (DNP-BSA) given at varying intervals after the injection of BSA. The results indicated that the helper activity was independent of DTH to the carrier protein, suggesting that these two activities, are mediated by different populations of functional cells. A low dose of tolerogenic soluble BSA (sBSA) was sufficient to abrogate the helper activity in the response to DNP-BSA. In contrast, DTH to BSA was only partially depressed by the pretreatment with a low dose of sBSA and was completely depressed by a high dose. DTH reactivity in mice pretreated with a low dose of tolerogen and followed by the immunization with BSA in Freund's complete adjuvant was substantiated by the microscopic observation of mononuclear cell infiltration at the site of the test antigen injection. These results suggest that cells involved in the helper function and DTH may be derived from different precursors.

Full text

PDF
241

Images in this article

242Figure 2
on p.242
242Figure 3
on p.242

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asherson G. L. Selective and specific inhibition of 24-hour skin reactions in the guinea-pig. II. The mechanism of immune deviation. Immunology. 1966 Feb;10(2):179–186. [PMC free article] [PubMed] [Google Scholar]
  2. Asherson G. L., Stone S. H. Selective and specific inhibition of 24 hour skin reactions in the guinea-pig. I. Immune deviation: description of the phenomenon and the effect of splenectomy. Immunology. 1965 Sep;9(3):205–217. [PMC free article] [PubMed] [Google Scholar]
  3. Borel Y., Fauconnet M., Miescher P. A. Selective suppression of delayed hypersensitivity by the induction of immunologic tolerance. J Exp Med. 1966 Apr 1;123(4):585–598. doi: 10.1084/jem.123.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dauphinee M. J., Nordin A. A. Studies of the immunological capacity of germ-free mouse radiation chimeras. IV. Cell-mediated immunity. Cell Immunol. 1974 Dec;14(3):394–401. doi: 10.1016/0008-8749(74)90189-0. [DOI] [PubMed] [Google Scholar]
  5. Dennert G., Lennox E. S. Cooperation and cell-mediated cytotoxicity as functions of two subsets of T cells. J Immunol. 1974 Nov;113(5):1553–1561. [PubMed] [Google Scholar]
  6. Elliott B. E., Haskill J. S., Axelrad M. A. Rosette-forming ability of thymus-derived lymphocytes in cell-mediated immunity. I. Delayed hypersensitivity and in vitro cytotoxicity. J Exp Med. 1975 Mar 1;141(3):584–599. doi: 10.1084/jem.141.3.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elliott B. E., Haskill J. S. Rosette-forming ability of thymus-derived lymphocytes in humoral and cell-mediated immunity. II. Helper cell activity. J Exp Med. 1975 Mar 1;141(3):600–607. doi: 10.1084/jem.141.3.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Katsura Y. Cell-mediated and humoral immune responses in mice. III. Dynamic balance between delayed-type hypersensitivity and antibody response. Immunology. 1977 Mar;32(3):227–235. [PMC free article] [PubMed] [Google Scholar]
  9. Katsura Y. Studies on M and G antibody response of mice to bovine serum albumin. II. Induction of tolerance. Jpn J Microbiol. 1972 Jul;16(4):269–274. doi: 10.1111/j.1348-0421.1972.tb00659.x. [DOI] [PubMed] [Google Scholar]
  10. Liew F. Y., Parish C. R. Lack of a correlation between cell-mediated immunity to the carrier and the carrier-hapten helper effect. J Exp Med. 1974 Mar 1;139(3):779–784. doi: 10.1084/jem.139.3.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mackaness G. B., Lagrange P. H., Miller T. E., Ishibashi T. Feedback inhibition of specifically sensitized lymphocytes. J Exp Med. 1974 Mar 1;139(3):543–559. doi: 10.1084/jem.139.3.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Muramatsu S., Amagai T., Katura Y. Tolerance induction in TxXBT and TxXB mice. Immunology. 1975 May;28(5):943–957. [PMC free article] [PubMed] [Google Scholar]
  13. Nordin A. A., Farrar J. J. Studies of the immunological capacity of germfree mouse radiation chimeras. III. In vitro reconstitution of the T-helper cell deficiency. Cell Immunol. 1974 Feb;10(2):218–225. doi: 10.1016/0008-8749(74)90113-0. [DOI] [PubMed] [Google Scholar]
  14. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  15. Segal S., Cohen I. R., Feldman M. Thymus-derived lymphocytes: humoral and cellular reactions distinguished by hydrocortisone. Science. 1972 Mar 10;175(4026):1126–1128. doi: 10.1126/science.175.4026.1126. [DOI] [PubMed] [Google Scholar]
  16. Silver J., Benacerraf B. Dissociation of T cell helper function and delayed hypersensitivity. J Immunol. 1974 Dec;113(6):1872–1875. [PubMed] [Google Scholar]
  17. Takaoki M., Kawaguchi S., Katsura Y., Muramatsu S. Transition in the character of immunological memory in mice after immunization. I. Memory for IgM and IgG antibody responses. Jpn J Microbiol. 1976 Aug;20(4):255–261. doi: 10.1111/j.1348-0421.1976.tb00986.x. [DOI] [PubMed] [Google Scholar]
  18. Tamura S. I., Egashira Y. Cellular and humoral immune responses in mice. II. Effect of intraperitoneal or subcutaneous injection of carrier of anti-hapten antibody and delayed hypersensitivity responses. Immunology. 1975 May;28(5):909–924. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES