Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1977 Oct;33(4):485–490.

IgG anti-hapten antibody secretion in vitro commences after extensive precursor proliferation.

J T Kemshead, J R North, B A Askonas
PMCID: PMC1445380  PMID: 336521

Abstract

We have investigated whether the intermediate cells that arise in primed mice after boosting require further cycles of division in culture before maturation into IgG antibody secreting cells. Killing of dividing cells between days 1--4 in culture, by exposure to BUdR-uv irradiation ablated the high IgG response observed on day 5 in control cultures. After T cell removal and replacement by a soluble factor (TRF) similar results were obtained. Thus B cell division over an extended period occurs prior to the appearance of IgG secreting cells. Furthermore, autoradiography of plaques from cultures briefly exposed to [3H]thymidine before harvest showed that some antibody secreting cells were synthesizing DNA at the time of assay.

Full text

PDF
490

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ada G. L., Humphrey J. H., Askonas B. A., McDevitt H. O., Nossal G. J. Correlation of grain counts with radioactivity (125I and tritium) in autoradiography. Exp Cell Res. 1966 Mar;41(3):557–572. doi: 10.1016/s0014-4827(66)80106-4. [DOI] [PubMed] [Google Scholar]
  2. Dutton R. W., Mishell R. I. Cell populations and cell proliferation in the in vitro response of normal mouse spleen to heterologous erythrocytes. Analysis by the hot pulse technique. J Exp Med. 1967 Sep 1;126(3):443–454. doi: 10.1084/jem.126.3.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hünig T., Schimpl A., Wecker E. Autoradiographic studies on the proliferation of antibody-producing cells in vitro. J Exp Med. 1974 Mar 1;139(3):754–760. doi: 10.1084/jem.139.3.754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kearney J. F., Lawton A. R. B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes. J Immunol. 1975 Sep;115(3):671–676. [PubMed] [Google Scholar]
  5. Kettman J., Yin E., Dutton R. W. Precursor cell division during the immune response in vitro: antigen-induced and "spontaneous" antibody-forming cells. Eur J Immunol. 1973 Oct;3(10):655–656. doi: 10.1002/eji.1830031014. [DOI] [PubMed] [Google Scholar]
  6. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. North J. R., Askonas B. A. IgG response in vitro. I. The requirement for an intermediate responsive cell type. Eur J Immunol. 1976 Jan;6(1):8–15. doi: 10.1002/eji.1830060104. [DOI] [PubMed] [Google Scholar]
  8. Sulitzeanu D., Marbrook J., Haskill J. S. Direct conversion of precursors of PFCs into active PFCs in vitro, without prior cell division. Immunology. 1973 Apr;24(4):707–710. [PMC free article] [PubMed] [Google Scholar]
  9. Zauderer M., Askonas B. A. Several proliferative phases precede maturation of IgG-secreting cells in mitogen-stimulated cultures. Nature. 1976 Apr 15;260(5552):611–613. doi: 10.1038/260611a0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES