Skip to main content
Immunology logoLink to Immunology
. 1977 Jul;33(1):101–107.

Detection and molecular characterization of the thymus-brain antigen in human brain.

R Arndt, R Stark, H G Thiele
PMCID: PMC1445415  PMID: 68924

Abstract

Anti-human brain sera were found to contain antibodies reacting with determinants present on murine thymocyte plasma membranes. This determinant is borne by the thymocyte-brain antigen of mouse thymocytes. The non species-specific determinant of the thymocyte-brain system is detectable on the thymocyte-brain antigen of human and mouse brain in comparable amounts. In contrast to these findings, the allogenic Thy-1 and the species-specific determinant of this system were only found on the thymocyte-brain antigen of mouse brain but not on the corresponding antigen of human brain. The molecular weight, Stokes radius and diffusion coefficient of the thymocyte-brain antigen of human and mouse brain are in good accordance with the data estimated for the corresponding antigen of murine thymocytes.

Full text

PDF
103

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arndt R., Stark R., Klein P., Müller A., Thiele H. G. Solubilization and molecular characterization of membrane-bound antigens shared by thymocytes and brain. Eur J Immunol. 1976 May;6(5):333–340. doi: 10.1002/eji.1830060506. [DOI] [PubMed] [Google Scholar]
  2. Barclay A. N., Letarte-Muirhead M., Williams A. F. Purification of the Thy-1 molecule from rat brain. Biochem J. 1975 Dec;151(3):699–706. doi: 10.1042/bj1510699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brouet J., Toben H. Characterization of a subpopulation of human T lymphocytes reactive with an heteroantiserum to human brain. J Immunol. 1976 Apr;116(4):1041–1044. [PubMed] [Google Scholar]
  4. Brown G., Greaves M. F. Cell surface markers for human T and B lymphocytes. Eur J Immunol. 1974 Apr;4(4):302–310. doi: 10.1002/eji.1830040414. [DOI] [PubMed] [Google Scholar]
  5. CRESTFIELD A. M., MOORE S., STEIN W. H. The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem. 1963 Feb;238:622–627. [PubMed] [Google Scholar]
  6. Doria G., Baroni C. D. Cross-reactivity between human thymus and mouse lymphoid tissues, as revealed by rabbit antiserum against human brain. Proc Soc Exp Biol Med. 1975 Apr;148(4):1126–1131. doi: 10.3181/00379727-148-38702. [DOI] [PubMed] [Google Scholar]
  7. Douglas T. C. Occurrence of a theta-like antigen in rats. J Exp Med. 1972 Nov 1;136(5):1054–1062. doi: 10.1084/jem.136.5.1054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Golub E. S. Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971 Aug;2(4):353–361. doi: 10.1016/0008-8749(71)90070-0. [DOI] [PubMed] [Google Scholar]
  9. Golub E. S. The distribution of brain-associated theta antigen cross-reactive with mouse in the brain of other species. J Immunol. 1972 Jul;109(1):168–170. [PubMed] [Google Scholar]
  10. Greaves M. F., Brown G. Letter: Antigenic correlations between brain and thymus. Lancet. 1974 Mar 16;1(7855):455–455. doi: 10.1016/s0140-6736(74)92409-x. [DOI] [PubMed] [Google Scholar]
  11. Kongshavn P. A., Gold P., Shuster J., Colquhoun B., Freedman S. O. Ability of anti-brain heteroantisera to distinguish thymus-derived lymphocytes in various species. Clin Immunol Immunopathol. 1974 Sep;3(1):1–15. doi: 10.1016/0090-1229(74)90019-1. [DOI] [PubMed] [Google Scholar]
  12. Letarte-Muirhead M., Barclay A. N., Williams A. F. Purification of the Thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes. Biochem J. 1975 Dec;151(3):685–697. doi: 10.1042/bj1510685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Micheel B., Pasternak G., Steuden J. Demonstration of theta-AKR differentiation antigen in rat tissue by mouse alloantiserum. Nat New Biol. 1973 Feb 14;241(111):221–222. doi: 10.1038/newbio241221a0. [DOI] [PubMed] [Google Scholar]
  14. Morris R. J., Letarte-Muirhead M., Williams A. F. Analysis in deoxycholate of three antigenic specificities associated with the rat Thy-1 molecule. Eur J Immunol. 1975 Apr;5(4):282–285. doi: 10.1002/eji.1830050413. [DOI] [PubMed] [Google Scholar]
  15. Morris R. J., Williams A. F. Antigens on mouse and rat lymphocytes recognized by rabbit antiserum against rat brain: the quantitative analysis of a xenogeneic antiserum. Eur J Immunol. 1975 Apr;5(4):274–281. doi: 10.1002/eji.1830050412. [DOI] [PubMed] [Google Scholar]
  16. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Takada A., Takada Y., Ito U., Minowada J. Shared antigenic determinants between human brain and human T-cell line. Clin Exp Immunol. 1974 Dec;18(4):491–498. [PMC free article] [PubMed] [Google Scholar]
  18. Thiele H. G., Stark R. Common antibody-induced redistribution on thymocytes of strain-, species- and non-species-specific antigenic determinants shared by brain and thymocytes of mice. Immunology. 1974 Nov;27(5):807–813. [PMC free article] [PubMed] [Google Scholar]
  19. Thiele H. G., Stark R., Földi M. Antigenic correlations between brain tissue and thymocytes in rats and mice. Naturwissenschaften. 1972 May;59(5):221–221. doi: 10.1007/BF00595522. [DOI] [PubMed] [Google Scholar]
  20. Thiele H. G., Stark R., Keeser D. Antigenic correlations between brain and thymus. I. Common antigenic structures in rat and mouse brain tissue and thymocytes. Eur J Immunol. 1972 Oct;2(5):424–429. doi: 10.1002/eji.1830020508. [DOI] [PubMed] [Google Scholar]
  21. Thiele H. G., Stark R., Keeser D., Zimpel H. Antigenic correlations between brain and thymus. II. Further evidence for the organ specificity of the thymus-brain system in mice. Z Immunitatsforsch Exp Klin Immunol. 1974 Feb;146(4):346–359. [PubMed] [Google Scholar]
  22. Thiele H. G., Stark R., Keeser D., Zimpel H. Letter: Lack of antigenic correlations between brain and thymus. Lancet. 1973 Dec 22;2(7843):1447–1447. doi: 10.1016/s0140-6736(73)92845-6. [DOI] [PubMed] [Google Scholar]
  23. Wick G., Schauenstein K. Letter: Lack of antigenic correlation between chicken brain and thymus. Lancet. 1974 Jul 27;2(7874):214–215. doi: 10.1016/s0140-6736(74)91507-4. [DOI] [PubMed] [Google Scholar]
  24. Zimpel H., Stark R., Thiele H. G. An approach to avoiding errors in cytotoxic testing. J Immunol Methods. 1973 Dec;3(4):411–416. doi: 10.1016/0022-1759(73)90043-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES