Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1977 Jun;32(6):975–980.

Nocardia water-soluble mitogen and lipopolysaccharide. Comparative study of two adjuvants and B-cell mitogens in mice.

I Lowy, C Bona, R Ciorbaru, L Chedid
PMCID: PMC1445450  PMID: 328390

Abstract

The activity of Nocardia water-soluble mitogen (NWSM) and LPS were compared in several experimental systems, since both compounds are B-cell mitogens and polyclonal activators in vitro. The results reported here demonstrated that NWSM like LPS also has a strong adjuvant activity in vivo if administered in saline with a strong antigen (heterologous red blood cells) or even with a weak immunogen such as theta alloantigen. However, in contrast to LPS, NWSM administered to mice failed to induce in vivo proliferation of lymphocytes, polyclonal activation and PFC against syngeneic bromelain-treated erythrocytes and thymocytes. It is possible therefore, that different mechanisms may be responsible for adjuvant activity of NWSM and LPS.

Full text

PDF
979

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Möller G., Sjöberg O. Selective induction of DNA synthesis in T and B lymphocytes. Cell Immunol. 1972 Aug;4(4):381–393. doi: 10.1016/0008-8749(72)90040-8. [DOI] [PubMed] [Google Scholar]
  2. Bona C., Chedid L., Damais C., Ciorbaru R., Shek P. N., Dubiski S., Cinader B. Blast transformation of rabbit B-derived lymphocytes by a mitogen extracted from Nocardia. J Immunol. 1975 Jan;114(1 Pt 2):348–353. [PubMed] [Google Scholar]
  3. Bona C., Damais C., Chedid L. Blastic transformtion of mouse spleen lymphocytes by a water-soluble mitogen extracted from Nocardia. Proc Natl Acad Sci U S A. 1974 May;71(5):1602–1606. doi: 10.1073/pnas.71.5.1602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chiller J. M., Skidmore B. J., Morrison D. C., Weigle W. O. Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2129–2133. doi: 10.1073/pnas.70.7.2129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ciorbaru R., Adam A., Petit J. F., Lederer E., Bona C., Chedid L. Isolation of mitogenic and adjuvant active fractions from various species of Nocardiae. Infect Immun. 1975 Feb;11(2):257–264. doi: 10.1128/iai.11.2.257-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ciorbaru R., Petit J. F., Lederer E., Zissman E., Bona C., Chedid L. Presence and subcellular localization of two distinct mitogenic fractions in the cells of Nocardia rubra and Nocardia opaca: preparation of soluble mitogenic peptidoglycan fractions. Infect Immun. 1976 Apr;13(4):1084–1090. doi: 10.1128/iai.13.4.1084-1090.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cunningham A. J. Large numbers of cells in normal mice produce antibody components of isologous erythrocytes. Nature. 1974 Dec 20;252(5485):749–751. doi: 10.1038/252749a0. [DOI] [PubMed] [Google Scholar]
  8. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  9. Fuji H., Zaleski M., Milgrom F. Immune response to alloantigens of thymus studied in mice with plaque assay. J Immunol. 1971 Jan;106(1):56–64. [PubMed] [Google Scholar]
  10. JOHNSON A. G., GAINES S., LANDY M. Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide. J Exp Med. 1956 Feb 1;103(2):225–246. doi: 10.1084/jem.103.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  12. Skidmore B. J., Chiller J. M., Morrison D. C., Weigle W. O. Immunologic properties of bacterial lipopolysaccharide (LPS): correlation between the mitogenic, adjuvant, and immunogenic activities. J Immunol. 1975 Feb;114(2 Pt 2):770–775. [PubMed] [Google Scholar]
  13. Skidmore B. J., Chiller J. M., Weigle W. O., Riblet R., Watson J. Immunologic properties of bacterial lipopolysaccharide (LPS). III. Genetic linkage between the in vitro mitogenic and in vivo adjuvant properties of LPS. J Exp Med. 1976 Jan 1;143(1):143–150. doi: 10.1084/jem.143.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES