Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1974 Jul;27(1):1–9.

Isoelectric focusing spectra of rabbit antibodies to Salmonella abortus-equi detected by anti-idiotypic sera

G Bordenave, Brigitte A Askonas
PMCID: PMC1445591  PMID: 4852579

Abstract

Serum antibodies from rabbits hyperimmunized with Salmonella abortus-equi (S.a-e) were examined by isoelectric focusing in polyacrylamide gel, followed by the coating of the gel with iodinated polysaccharide extracted from bacterial cell walls. The antibody populations in these sera were highly heterogeneous. Anti-idiotypic sera, prepared in allotypically matched rabbits, recognized only a fraction of these antibody populations (generally from one to three clonal antibodies per serum). Different rabbits subjected to the same anti-idiotypic immunization can recognize different antibodies in the same serum to S.a-e.

Homologous and heterologous idiotypes recognized by the same anti-idiotypic serum have shown very similar but never identical pI spectra. It appears that the same group of idiotypic determinants can be markers for several clonal antibodies and are not unique to a single clone.

Two rabbits were studied throughout an immunization course lasting 2 years; we did not observe any change in the clonal product of antibody-producing cells carrying the idiotypic determinants.

Full text

PDF
7

Images in this article

3FIG. 1
on p.3
4FIG. 2
on p.4
5FIG. 3
on p.5
6FIG. 4
on p.6
7FIG. 5
on p.7

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., Williamson A. R., Wright B. E. Selection of a single antibody-forming cell clone and its propagation in syngeneic mice. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1398–1403. doi: 10.1073/pnas.67.3.1398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Awdeh Z. L., Williamson A. R., Askonas B. A. One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins. Biochem J. 1970 Jan;116(2):241–248. doi: 10.1042/bj1160241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyer S. H. Extraordinary incidence of electrophoretically silent genetic polymorphisms. Nature. 1972 Oct 20;239(5373):453–454. doi: 10.1038/239453a0. [DOI] [PubMed] [Google Scholar]
  4. Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Anal Biochem. 1967 Jul;20(1):150–154. doi: 10.1016/0003-2697(67)90272-2. [DOI] [PubMed] [Google Scholar]
  5. Daugharty H., Hopper J. E., MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. I. Analysis of precipitating antibody populations. J Exp Med. 1969 Nov 1;130(5):1047–1062. doi: 10.1084/jem.130.5.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eichmann K., Braun D. G., Feizi T., Krause R. M. The emergence of antibodies with either identical or unrelated individual antigenic specificity during repeated immunizations with streptococcal vaccines. J Exp Med. 1970 Jun 1;131(6):1169–1189. doi: 10.1084/jem.131.6.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Freeman G. G. The preparation and properties of a specific polysaccharide from Bact. typhosum Ty(2): With an addendum by J. St L. Philpot, From the Department of Biochemistry, Oxford. Biochem J. 1942 Apr;36(3-4):340–356. doi: 10.1042/bj0360340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kindt T. J., Klapper D. G., Waterfield M. D. An idiotypic cross-reaction between allotype a3 and allotype a negative rabbit antibodies to streptococcal carbohydrate. J Exp Med. 1973 Mar 1;137(3):636–648. doi: 10.1084/jem.137.3.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. 3. Persistence and variations of idiotypic specificities during the course of immunization. J Exp Med. 1970 Mar 1;131(3):583–601. doi: 10.1084/jem.131.3.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitchell G. F., Humphrey J. H., Wiliamson A. R. Inhibition of secondary anti-hapten responses with the hapten conjugated to type 3 pneumococcal polysaccharide. Eur J Immunol. 1972 Oct;2(5):460–467. doi: 10.1002/eji.1830020516. [DOI] [PubMed] [Google Scholar]
  11. Nisonoff A., Macdonald A. B., Hopper J. E., Daugharty H. Quantitative studies of idiotypic antibodies. Fed Proc. 1970 Jan-Feb;29(1):72–77. [PubMed] [Google Scholar]
  12. OUDIN J., MICHEL M. [A new allotype form of rabbit serum gamma-globulins, apparently associated with antibody function and specificity]. C R Hebd Seances Acad Sci. 1963 Jul 17;257:805–808. [PubMed] [Google Scholar]
  13. Oudin J., Cazenave P. A. Similar idiotypic specificities in immunoglobulin fractions with different antibody functions or even without detectable antibody function. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2616–2620. doi: 10.1073/pnas.68.10.2616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oudin J., Michel M. Idiotypy of rabbit antibodies. II. Comparison of idiotypy of various kinds of antibodies formed in the same rabbits against Salmonella typhi. J Exp Med. 1969 Sep 1;130(3):619–642. doi: 10.1084/jem.130.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Williamson A. R. Antibody isoelectric spectra. Analysis of the heterogeneity of antibody molecules in serum by isoelectric focusing in gel and specific detection with hapten. Eur J Immunol. 1971 Nov;1(5):390–394. doi: 10.1002/eji.1830010517. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES