Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1974 Oct;27(4):577–587.

Cytophilic activity of IgG2 from sera of unimmunized guinea-pigs

R G Q Leslie, S Cohen
PMCID: PMC1445709  PMID: 4435833

Abstract

The interaction between 7S IgG2 from unimmunized guinea-pigs and macrophage-rich peritoneal exudate cells from oil-stimulated guinea-pigs was examined by 125I-labelled IgG2 uptake.

The cell uptake of 125I-labelled IgG2 reaches a maximum after 90 minutes at 20°; increased levels of labelling (1 to 23 atoms of iodine per immunoglobulin molecule) lead to slightly enhanced uptake, but the effect does not exceed experimental variability.

Peripheral white blood cells bind relatively low levels of IgG2; the uptake by peritoneal cells is attributable primarily to a subpopulation of glass-adherent cells, presumably macrophages.

Binding of IgG2 to macrophages is reversible even with a mild washing procedure. Repeated washing of cells did not affect the association constant, but the apparent number of receptor sites per cell was progressively reduced. After correction for the washing procedure employed, the number of IgG2 receptor sites was estimated as 2.5±0.4 x 106 per cell; the association constant (Ka) was 1.46±0.45 x 106 L/M at 20°.

Full text

PDF
587

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arend W. P., Mannik M. The macrophage receptor for IgG: number and affinity of binding sites. J Immunol. 1973 Jun;110(6):1455–1463. [PubMed] [Google Scholar]
  2. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berken A., Benacerraf B. Properties of antibodies cytophilic for macrophages. J Exp Med. 1966 Jan 1;123(1):119–144. doi: 10.1084/jem.123.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Del Guercio P., Tolone G., Andrade F. B., Biozzi G., Binaghi R. A. Opsonic, cytophilic and agglutinating activity of guinea-pig gamma-2 and gamma-M anti-Salmonella antibodies. Immunology. 1969 Mar;16(3):361–371. [PMC free article] [PubMed] [Google Scholar]
  6. Dobson C., Morseth D. J., Soulsby J. L. Immunoglobulin E-type antibodies induced by Ascaris suum infections in guinea pigs. J Immunol. 1971 Jan;106(1):128–133. [PubMed] [Google Scholar]
  7. Gowland E. The physico-chemical properties of cytophilic antibody. Aust J Exp Biol Med Sci. 1968 Feb;46(1):73–81. doi: 10.1038/icb.1968.6. [DOI] [PubMed] [Google Scholar]
  8. Lay W. H., Nussenzweig V. Ca++-dependent binding of antigen-19 S antibody complexes to macrophages. J Immunol. 1969 May;102(5):1172–1178. [PubMed] [Google Scholar]
  9. Leslie R. G., Cohen S. Chemical properties of guinea-pig immunoglobulins gamma-1 G, gamma-2 G and gamma M. Biochem J. 1970 Dec;120(4):787–795. doi: 10.1042/bj1200787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leslie R. G., Cohen S. Cytophilic activity of IgG2 from sera of guinea-pigs immunized with bovine gamma-globulin. Immunology. 1974 Oct;27(4):589–599. [PMC free article] [PubMed] [Google Scholar]
  11. Leslie R. G., Melamed M. D., Cohen S. The products from papain and pepsin hydrolyses of guinea-pig immunoglobulins gamma 1G and gamma 2 G. Biochem J. 1971 Mar;121(5):829–837. doi: 10.1042/bj1210829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Liew F. Y. The binding of the Fc fragment of guinea-pig cytophilic antibody to peritoneal macrophages. Immunology. 1971 May;20(5):817–829. [PMC free article] [PubMed] [Google Scholar]
  13. Linscott W. D., Hansen S. S. Non-complement-fixing IgM antibodies in the guinea pig. J Immunol. 1969 Sep;103(3):423–428. [PubMed] [Google Scholar]
  14. Nelson D. S., Mildenhall P. Studies on cytophilic antibodies. II. The production by guinea-pigs of macrophage cytophilic antibodies to sheep erythrocytes and human serum albumin: relationship to the production of other antibodies and the development of delayed-type hypersensitivity. Aust J Exp Biol Med Sci. 1968 Feb;46(1):33–49. [PubMed] [Google Scholar]
  15. OVARY Z., BENACERRAF B., BLOCH K. J. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis. J Exp Med. 1963 Jun 1;117:951–964. doi: 10.1084/jem.117.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reisfeld R. A., Hyslop N. E. Separation of 7S gamma-1-globulin, 7S gamma-2-globulin and macroglobulin guinea pig antibodies. Proc Soc Exp Biol Med. 1966 Feb;121(2):508–514. doi: 10.3181/00379727-121-30817. [DOI] [PubMed] [Google Scholar]
  17. Sandberg A. L., Oliveira B., Osler A. G. Two complement interaction sites in guinea pig immunoglobulins. J Immunol. 1971 Jan;106(1):282–285. [PubMed] [Google Scholar]
  18. Sandberg A. L., Osler A. G., Shin H. S., Oliveira B. The biologic activities of guinea pig antibodies. II. Modes of complement interaction with gamma 1 and gamma 2-immunoglobulins. J Immunol. 1970 Feb;104(2):329–334. [PubMed] [Google Scholar]
  19. Shortman K., Williams N., Jackson H., Russell P., Byrt P., Diener E. The separation of different cell classes from lymphoid organs. IV. The separation of lymphocytes from phagocytes on glass bead columns, and its effect on subpopulations of lymphocytes and antibody-forming cells. J Cell Biol. 1971 Mar;48(3):566–579. doi: 10.1083/jcb.48.3.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strejan G., Campbell D. H. Hypersensitivity to Ascaris antigens. II. The skin-sensitizing properties of 7 S-gamma-2 antibody from sensitized guinea pigs as tested in guinea pigs. J Immunol. 1967 Aug;99(2):347–356. [PubMed] [Google Scholar]
  21. Strejan G., Campbell D. H. Skin-sensitizing properties of guinea pig antibodies to keyhole limpet hemocyanin. J Immunol. 1968 Jun;100(6):1245–1254. [PubMed] [Google Scholar]
  22. Vaerman J. P., Heremans J. F. The IgA system of the guinea pig. J Immunol. 1972 Mar;108(3):637–648. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES