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. 1975 Jan;28(1):59–70.

The effect of various immunosuppressive agents on mouse peritoneal macrophages and on the in vitro phagocytosis of Escherichia coli O4:K3:H5 and degradation of 125I-labelled HSA-antibody complexes by these cells.

O V Gadeberg, J M Rhodes, S O Larsen
PMCID: PMC1445766  PMID: 1090520

Abstract

Large doses of hydrocortisone, cyclophosphamide, and methotrexate injected subcutaneously, and whole-body irradiation (500 rads) caused a reduction in the number of peritoneal cells (PE cells) obtained after intraperitoneal injection of the treated mice with proteose-peptone. The same dose of cyclophosphamide and irradiation induced morphological changes in PE macrophages. There were more giant cells in the peritoneal exudates from treated mice as compared to control mice. 'Pharmacological' and larger doses of hydrocortisone, methotrexate and azathioprine or anti-lymphocyte globulin had no effect on the in vitro phagocytic capacity of proteose-peptone-stimulated mouse PE macrophages. This also applied to doses of up to 50 mg/kg of cyclophosphamide. In contrast, whole-body irradiation (500 rad) and 100 mg/kg of cyclophosphamide decreased the phagocytic capacity of mouse macrophages in vitro and reduced the ability of PE cells to degrade 125I-labelled HSA-antibody complexes in vitro. The greatest effect was noted 4-5 days after whole-body irradiation or four to five subcutaneous injections of cyclophosphamide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK L., BERENBAUM M. C. FACTORS AFFECTING THE DYE EXCLUSION TEST FOR CELL VIABILITY. Exp Cell Res. 1964 Jun;35:9–13. doi: 10.1016/0014-4827(64)90066-7. [DOI] [PubMed] [Google Scholar]
  2. BREWER H. B., Jr, COMSTOCK J. P., ARONOW L. Effects of nitrogen mustard on protein and nucleic acid synthesis in mouse fibroblasts growing in vitro. Biochem Pharmacol. 1961 Nov;8:281–287. doi: 10.1016/0006-2952(61)90102-2. [DOI] [PubMed] [Google Scholar]
  3. Castaldi G., Zavagli G., Fiocchi O., Trotta F. Giant histiocytes after cyclophosphamide. Experientia. 1970 Mar 15;26(3):300–301. doi: 10.1007/BF01900109. [DOI] [PubMed] [Google Scholar]
  4. Doré C. F., Balfour B. M. A device for preparing cell spreads. Immunology. 1965 Oct;9(4):403–405. [PMC free article] [PubMed] [Google Scholar]
  5. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  6. Kornfeld L., Greenman V. Effects of total-body x-irradiation on peritoneal cells of mice. Radiat Res. 1966 Nov;29(3):433–444. [PubMed] [Google Scholar]
  7. NELSON E. L., BECKER J. R. The effect of whole-body x irradiation on the bactericidal activity of phagocytic cells. I. Survival of Pseudomonas aeruginosa within phagocytes from peritoneal exudates of mice. J Infect Dis. 1959 Jan-Feb;104(1):13–19. doi: 10.1093/infdis/104.1.13. [DOI] [PubMed] [Google Scholar]
  8. PUCK T. T., MARCUS P. I. Action of x-rays on mammalian cells. J Exp Med. 1956 May 1;103(5):653–666. doi: 10.1084/jem.103.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SORKIN E., BOYDEN S. V. Studies on the fate of antigens in vitro. I. The effect of specific antibody on the fate of I 131 trace labeled human serum albumin in vitro in the presence of guinea pig monocytes. J Immunol. 1959 Apr;82(4):332–339. [PubMed] [Google Scholar]
  10. Thompson J., van Furth R. The effect of glucocorticosteroids on the kinetics of mononuclear phagocytes. J Exp Med. 1970 Mar 1;131(3):429–442. doi: 10.1084/jem.131.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wiener E., Marmary Y., Curelaru Z. The in vitro effect of hydrocortisone on the uptake and intracellular digestion of particulate matter by macrophages in culture. Lab Invest. 1972 Feb;26(2):220–226. [PubMed] [Google Scholar]
  12. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

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