Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1975 Apr;28(4):635–651.

A study of idiotypic suppression in adult rabbits immunized with Salmonella abortus-equi.

G R Bordenave
PMCID: PMC1445840  PMID: 50283

Abstract

It is possible to induce idiotypic suppression in adult rabbits immunized with Salmonella abortus-equi (S.a.e.). Ten months after priming we injected the rabbit with anti-idiotypic serum prepared against its own antibodies to S.a.e. and, 3 weeks later, gave it a booster injection of bacteria. A new anti-idiotypic serum was preparedwith the serum to S.a.e. collected after this boost and was used for the following isiotypic suppression attempt made 10 months after the first one. Using this procedurewe succeeded in two successive idiotypic suppression attempts in the same rabbit. Inthe three attempts we carried out, idiotypic suppression was totally effective, i.e. idiotypes detected by the serum used for the suppression totally disappeared after thesuppression, and the suppression lasted during the life of the rabbits (maximum 10 months). This observation is consistent with a suppression resulti-g from an interaction of anti-idiotypic antibodies with the complementary receptors at the surface of memory cells. This suppression was without effect on antibody to S.a.e. titre and on IgG concentration. Idiotypes detected by the anti-idiotypic serum prepared withthe serum to S.a.e. collected after the suppression. These idiotypes were different from those detected by the anti-idiotypic serum used for the suppression. This observation confirms that idiotypic recognition is confined to a limited number of clonal products, despite the fact that a very heterogeneous antibody population was used forthe anti-idiotypic immunization. Thus we did not observe the appearance of new idiotypes produced previously silent cell clones. All the different idiotypes we detected during the successive idiotypic suppression attempts carried determinants shich remained peculiar to each individual rabbit.

Full text

PDF
639

Images in this article

639FIG. 1
on p.639
639FIG. 2
on p.639
640FIG. 3
on p.640
642FIG. 4
on p.642
643FIG. 5
on p.643
644FIG. 6
on p.644
647FIG. 7
on p.647

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., Williamson A. R. Dominance of a cell clone forming antibody to DNP. Nature. 1972 Aug 11;238(5363):339–341. doi: 10.1038/238339a0. [DOI] [PubMed] [Google Scholar]
  2. Askonas B. A., Williamson A. R., Wright B. E. Selection of a single antibody-forming cell clone and its propagation in syngeneic mice. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1398–1403. doi: 10.1073/pnas.67.3.1398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Awdeh Z. L., Williamson A. R., Askonas B. A. Isoelectric focusing in polyacrylamide gel and its application to immunoglobulins. Nature. 1968 Jul 6;219(5149):66–67. doi: 10.1038/219066a0. [DOI] [PubMed] [Google Scholar]
  4. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  5. Bordenave G., Askonas B. A. Isoelectric focusing spectra of rabbit antibodies to Salmonella abortus-equi detected by anti-idiotypic sera. Immunology. 1974 Jul;27(1):1–9. [PMC free article] [PubMed] [Google Scholar]
  6. Cosenza H., Köhler H. Specific suppression of the antibody response by antibodies to receptors. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2701–2705. doi: 10.1073/pnas.69.9.2701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DRAY S. Effect of maternal isoantibodies on the quantitative expression of two allelic genes controlling gamma-globulin allotypic specificities. Nature. 1962 Aug 18;195:677–680. doi: 10.1038/195677a0. [DOI] [PubMed] [Google Scholar]
  8. Freeman G. G. The preparation and properties of a specific polysaccharide from Bact. typhosum Ty(2): With an addendum by J. St L. Philpot, From the Department of Biochemistry, Oxford. Biochem J. 1942 Apr;36(3-4):340–356. doi: 10.1042/bj0360340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  10. Hart D. A., Pawlak L. L., Nisonoff A. Nature of anti-hapten antibodies arising after immune suppression of a set of cross-raactive idiotypic specificities. Eur J Immunol. 1973 Jan;3(1):44–48. doi: 10.1002/eji.1830030110. [DOI] [PubMed] [Google Scholar]
  11. Hart D. A., Wang A. L., Pawlak L. L., Nisonoff A. Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody. J Exp Med. 1972 Jun 1;135(6):1293–1300. doi: 10.1084/jem.135.6.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herzenberg L. A., Jones P. P., Herzenberg L. A. Mouse immunoglobulin allotypes: characterization and use in cellular immunology. Ann Immunol (Paris) 1974 Jan;125C(1-2):71–83. [PubMed] [Google Scholar]
  13. KABAT E. A., MCDUFFIE F. C. A comparative study of methods used for analysis of specific precipitates in quantitative immunochemistry. J Immunol. 1956 Sep;77(3):193–197. [PubMed] [Google Scholar]
  14. LEVY H. B., SOBER H. A. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960 Jan;103:250–252. doi: 10.3181/00379727-103-25476. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lee W., Cosenza H., Köhler H. Clonal restriction of the immune response to phosphorylcholine. Nature. 1974 Jan 4;247(5435):55–57. doi: 10.1038/247055a0. [DOI] [PubMed] [Google Scholar]
  17. MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. 3. Persistence and variations of idiotypic specificities during the course of immunization. J Exp Med. 1970 Mar 1;131(3):583–601. doi: 10.1084/jem.131.3.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mitchell G. F., Humphrey J. H., Wiliamson A. R. Inhibition of secondary anti-hapten responses with the hapten conjugated to type 3 pneumococcal polysaccharide. Eur J Immunol. 1972 Oct;2(5):460–467. doi: 10.1002/eji.1830020516. [DOI] [PubMed] [Google Scholar]
  19. OUDIN J. Allotypy of rabbit serum proteins. I. Immuno-chemical analysis leading to the individualization of seven main allotypes. J Exp Med. 1960 Jul 1;112:107–124. doi: 10.1084/jem.112.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. OUDIN J. Allotypy of rabbit serum proteins. II. Relationships between various allotypes: their common antigenic specificity, their distribution in a sample population; genetic implications. J Exp Med. 1960 Jul 1;112:125–142. doi: 10.1084/jem.112.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. OUDIN J. Immunochemical analysis of human serum and its fractions. II. Qualitative and quantitative analysis of the fraction soluble in two-thirds saturated ammonium sulphate. J Immunol. 1958 Nov;81(5):376–388. [PubMed] [Google Scholar]
  22. OUDIN J. L'allotypie de certains antigènes protéidiques du sérum. C R Hebd Seances Acad Sci. 1956 May 23;242(21):2606–2608. [PubMed] [Google Scholar]
  23. OUDIN J., MICHEL M. [A new allotype form of rabbit serum gamma-globulins, apparently associated with antibody function and specificity]. C R Hebd Seances Acad Sci. 1963 Jul 17;257:805–808. [PubMed] [Google Scholar]
  24. OUDIN J. Réaction de précipitation spécifique entre des sérums d'animaux de même espèce. C R Hebd Seances Acad Sci. 1956 May 14;242(20):2489–2490. [PubMed] [Google Scholar]
  25. OUDIN J. [Allotypes of certain serum protein antigens. Immuno-chemical and genetic relationships between 6 principal allotypes observed in rabbit serum]. C R Hebd Seances Acad Sci. 1960 Jan 25;250:770–772. [PubMed] [Google Scholar]
  26. Oudin J., Michel M. Idiotypy of rabbit antibodies. I. Comparison of idiotypy of antibodies against Salmonella typhi with that of antibodies against other bacteria in the same rabbits, or of antibodies against Salmonella typhi in various rabbits. J Exp Med. 1969 Sep 1;130(3):595–617. doi: 10.1084/jem.130.3.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Oudin J., Michel M. Idiotypy of rabbit antibodies. II. Comparison of idiotypy of various kinds of antibodies formed in the same rabbits against Salmonella typhi. J Exp Med. 1969 Sep 1;130(3):619–642. doi: 10.1084/jem.130.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Oudin J., Michel M. Sur les spécificités idiotypiques des anticorps de lapin anti-S. typhi. C R Acad Sci Hebd Seances Acad Sci D. 1969 Jan 6;268(1):230–233. [PubMed] [Google Scholar]
  29. Oudin J. The genetic control of immunoglobulin synthesis. Proc R Soc Lond B Biol Sci. 1966 Nov 22;166(1003):207–221. doi: 10.1098/rspb.1966.0094. [DOI] [PubMed] [Google Scholar]
  30. Pawlak L. L., Hart D. A., Nisonoff A. Requirements for prolonged suppression of an idiotypic specificity in adult mice. J Exp Med. 1973 Jun 1;137(6):1442–1458. doi: 10.1084/jem.137.6.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Pawlak L. L., Hart D. A., Nisonoff A. Suppression of immunological memory for a cross-reactive idiotype in adult mice. Eur J Immunol. 1974 Jan;4(1):10–13. doi: 10.1002/eji.1830040104. [DOI] [PubMed] [Google Scholar]
  32. ROWLEY D. A., FITCH F. W. HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT. J Exp Med. 1964 Dec 1;120:987–1005. doi: 10.1084/jem.120.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Rowley D. A., Fitch F. W., Stuart F. P., Köhler H., Cosenza H. Specific suppression of immune responses. Science. 1973 Sep 21;181(4105):1133–1141. doi: 10.1126/science.181.4105.1133. [DOI] [PubMed] [Google Scholar]
  34. SPIEGELBERG H. L., WEIGLE W. O. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS. J Exp Med. 1965 Mar 1;121:323–338. doi: 10.1084/jem.121.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES