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. 1986 Aug;58(4):627–634.

Functional characteristics of intraepithelial lymphocytes from mouse small intestine. II. In vivo and in vitro responses of intraepithelial lymphocytes to mitogenic and allogeneic stimuli.

A M Mowat, S MacKenzie, M E Baca, M V Felstein, D M Parrott
PMCID: PMC1453117  PMID: 2942464

Abstract

Although isolated intraepithelial lymphocytes (IEL) have been shown to have specific and non-specific cytolytic functions, their ability to proliferate in response to T-cell mitogens or alloantigens is controversial. Here we show that IEL from mouse small intestine do not respond to mitogens such as concanavalin A and phytohaemagglutinin A or in mixed lymphocyte reactions unless an accessory spleen cell is also present. Adherent spleen cells possess the most potent helper function, but a dividing accessory cell may also be required. Supernatants from stimulated lymphocytes also assist IEL to proliferate in vitro, particularly in the presence of adherent accessory cells. IEL could not mediate lethal graft-versus-host disease in irradiated hosts, but could produce popliteal lymph node hypertrophy or splenomegaly in unirradiated hosts. Thus, IEL have the potential for proliferative activities characteristic of T cells, but they require accessory cells and/or factors such as interleukin-2 for their function in vitro and in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnaud-Battandier F., Bundy B. M., O'Neill M., Bienenstock J., Nelson D. L. Cytotoxic activities of gut mucosal lymphoid cells in guinea pigs. J Immunol. 1978 Sep;121(3):1059–1065. [PubMed] [Google Scholar]
  2. Arnaud-Battandier F., Nelson D. L. Immunologic characteristics of intestinal lymphoid cells of the guinea pig. Gastroenterology. 1982 Feb;82(2):248–253. [PubMed] [Google Scholar]
  3. Cerf-Bensussan N., Schneeberger E. E., Bhan A. K. Immunohistologic and immunoelectron microscopic characterization of the mucosal lymphocytes of human small intestine by the use of monoclonal antibodies. J Immunol. 1983 Jun;130(6):2615–2622. [PubMed] [Google Scholar]
  4. Davies M. D., Parrott D. M. Cytotoxic T cells in small intestine epithelial, lamina propria and lung lymphocytes. Immunology. 1981 Oct;44(2):367–371. [PMC free article] [PubMed] [Google Scholar]
  5. Davies M. D., Parrott D. M. Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine. Gut. 1981 Jun;22(6):481–488. doi: 10.1136/gut.22.6.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dillon S. B., MacDonald T. T. Functional properties of lymphocytes isolated from murine small intestinal epithelium. Immunology. 1984 Jul;52(3):501–509. [PMC free article] [PubMed] [Google Scholar]
  7. Farrar J. J., Fuller-Farrar J., Simon P. L., Hilfiker M. L., Stadler B. M., Farrar W. L. Thymoma production of T cell growth factor (Interleukin 2). J Immunol. 1980 Dec;125(6):2555–2558. [PubMed] [Google Scholar]
  8. Ferguson A. Intraepithelial lymphocytes of the small intestine. Gut. 1977 Nov;18(11):921–937. doi: 10.1136/gut.18.11.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferguson A., McClure J. P., Townley R. R. Intraepithelial lymphocyte counts in small intestinal biopsies from children with diarrhoea. Acta Paediatr Scand. 1976 Sep;65(5):541–546. doi: 10.1111/j.1651-2227.1976.tb04929.x. [DOI] [PubMed] [Google Scholar]
  10. Fiocchi C., Hilfiker M. L., Youngman K. R., Doerder N. C., Finke J. H. Interleukin 2 activity of human intestinal mucosa mononuclear cells. Decreased levels in inflammatory bowel disease. Gastroenterology. 1984 Apr;86(4):734–742. [PubMed] [Google Scholar]
  11. Flexman J. P., Shellam G. R., Mayrhofer G. Natural cytotoxicity, responsiveness to interferon and morphology of intra-epithelial lymphocytes from the small intestine of the rat. Immunology. 1983 Apr;48(4):733–741. [PMC free article] [PubMed] [Google Scholar]
  12. Ford W. L., Burr W., Simonsen M. A lymph node weight assay for the graft-versus-host activity of rat lymphoid cells. Transplantation. 1970 Sep;10(3):258–266. doi: 10.1097/00007890-197009000-00007. [DOI] [PubMed] [Google Scholar]
  13. Greenwood J. H., Austin L. L., Dobbins W. O., 3rd In vitro characterization of human intestinal intraepithelial lymphocytes. Gastroenterology. 1983 Nov;85(5):1023–1035. [PubMed] [Google Scholar]
  14. Guy-Grand D., Griscelli C., Vassalli P. The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978 Dec 1;148(6):1661–1677. doi: 10.1084/jem.148.6.1661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klein J. R., Kagnoff M. F. Nonspecific recruitment of cytotoxic effector cells in the intestinal mucosa of antigen-primed mice. J Exp Med. 1984 Dec 1;160(6):1931–1936. doi: 10.1084/jem.160.6.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lyscom N., Brueton M. J. Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies. Immunology. 1982 Apr;45(4):775–783. [PMC free article] [PubMed] [Google Scholar]
  17. Marsh M. N. Studies of intestinal lymphoid tissue. III. Quantitative analyses of epithelial lymphocytes in the small intestine of human control subjects and of patients with celiac sprue. Gastroenterology. 1980 Sep;79(3):481–492. [PubMed] [Google Scholar]
  18. Mayrhofer G., Whately R. J. Granular intraepithelial lymphocytes of the rat small intestine. I. Isolation, presence in T lymphocyte-deficient rats and bone marrow origin. Int Arch Allergy Appl Immunol. 1983;71(4):317–327. doi: 10.1159/000233414. [DOI] [PubMed] [Google Scholar]
  19. Mowat A. M., Tait R. C., MacKenzie S., Davies M. D., Parrott D. M. Analysis of natural killer effector and suppressor activity by intraepithelial lymphocytes from mouse small intestine. Clin Exp Immunol. 1983 Apr;52(1):191–198. [PMC free article] [PubMed] [Google Scholar]
  20. Nauss K. M., Pavlina T. M., Kumar V., Newberne P. M. Functional characteristics of lymphocytes isolated from the rat large intestine. Response to T-cell mitogens and natural killer cell activity. Gastroenterology. 1984 Mar;86(3):468–475. [PubMed] [Google Scholar]
  21. Parrott D. M., Tait C., MacKenzie S., Mowat A. M., Davies M. D., Micklem H. S. Analysis of the effector functions of different populations of mucosal lymphocytes. Ann N Y Acad Sci. 1983 Jun 30;409:307–320. doi: 10.1111/j.1749-6632.1983.tb26879.x. [DOI] [PubMed] [Google Scholar]
  22. Persson U., Hammarström L., Möller E., Möller G., Smith C. I. The role of adherent cells in B and T lymphocyte activation. Immunol Rev. 1978;40:78–101. doi: 10.1111/j.1600-065x.1978.tb00402.x. [DOI] [PubMed] [Google Scholar]
  23. Phillips A. D., Rice S. J., France N. E., Walker-Smith J. A. Small intestinal intraepithelial lymphocyte levels in cow's milk protein intolerance. Gut. 1979 Jun;20(6):509–512. doi: 10.1136/gut.20.6.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SIMONSEN M. Graft versus host reactions. Their natural history, and applicability as tools of research. Prog Allergy. 1962;6:349–467. [PubMed] [Google Scholar]
  25. Selby W. S., Janossy G., Jewell D. P. Immunohistological characterisation of intraepithelial lymphocytes of the human gastrointestinal tract. Gut. 1981 Mar;22(3):169–176. doi: 10.1136/gut.22.3.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shields J. G., Parrott D. M. Appearance of delayed-type hypersensitivity effector cells in murine gut mucosa. Immunology. 1985 Apr;54(4):771–776. [PMC free article] [PubMed] [Google Scholar]
  27. Tagliabue A., Befus A. D., Clark D. A., Bienenstock J. Characteristics of natural killer cells in the murine intestinal epithelium and lamina propria. J Exp Med. 1982 Jun 1;155(6):1785–1796. doi: 10.1084/jem.155.6.1785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tagliabue A., Luini W., Soldateschi D., Boraschi D. Natural killer activity of gut mucosal lymphoid cells in mice. Eur J Immunol. 1981 Nov;11(11):919–922. doi: 10.1002/eji.1830111112. [DOI] [PubMed] [Google Scholar]
  29. Wilders M. M., Sminia T., Janse E. M. Ontogeny of non-lymphoid and lymphoid cells in the rat gut with special reference to large mononuclear Ia-positive dendritic cells. Immunology. 1983 Oct;50(2):303–314. [PMC free article] [PubMed] [Google Scholar]

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