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. 1985 Apr;54(4):777–784.

Isolation of follicular dendritic cells from human tonsils and adenoids. III. Analysis of their Fc receptors.

E Heinen, D Radoux, C Kinet-Denoel, M Moeremans, J De Mey, L J Simar
PMCID: PMC1453568  PMID: 3156811

Abstract

Follicular dendritic cells (FDC), isolated from human tonsils or adenoids, were tested for their capacity to retain monomeric, aggregated or antigen-bound human antibodies in the absence of serum. FDC retain fluorescein-labelled heat-aggregated human immunoglobulins, but not monomeric ones nor fluorescein-labelled F(ab')2 in monomeric or aggregated form. Ultrastructural observations showed that colloidal gold-labelled monomeric, or antigen-bound, antibodies directed against tetanus toxoid are retained by dendrites and membrane infoldings of FDC but are never located in cytoplasmic vesicles. This retention was inhibited by incubating FDC with unlabelled aggregated or antigen-bound antibodies. When gold-labelled anti-tetanus toxoid antibodies were incubated in the presence of protein-A before the contact with FDC, a strong reduction of their retention occurred. This further suggested the presence of Fc receptors on isolated tonsillar FDC. Endocytosis was not observed in isolated FDC, even after prolonged incubation in presence of labelled immune complexes: their Fc receptors are, thus, not related to a phagocytic activity as they are in macrophages. Simultaneous ultrastructural labelling of Fc and C3b receptors with colloidal gold particles of different sizes did not reveal any clear relations between these two receptors on the surface of FDC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen L. L., Frank A. M., Adams J. C., Steinman R. M. Distribution of horseradish peroxidase (HRP)-anti-HRP immune complexes in mouse spleen with special reference to follicular dendritic cells. J Cell Biol. 1978 Oct;79(1):184–199. doi: 10.1083/jcb.79.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Curran R. C., Gregory J., Jones E. L. The distribution of immunoglobulin and other plasma proteins in human reactive lymph nodes. J Pathol. 1982 Apr;136(4):307–332. doi: 10.1002/path.1711360406. [DOI] [PubMed] [Google Scholar]
  3. Davies D. R., Metzger H. Structural basis of antibody function. Annu Rev Immunol. 1983;1:87–117. doi: 10.1146/annurev.iy.01.040183.000511. [DOI] [PubMed] [Google Scholar]
  4. Fearon D. T., Wong W. W. Complement ligand-receptor interactions that mediate biological responses. Annu Rev Immunol. 1983;1:243–271. doi: 10.1146/annurev.iy.01.040183.001331. [DOI] [PubMed] [Google Scholar]
  5. Gerdes J., Stein H. Complement (C3) receptors on dendritic reticulum cells of normal and malignant lymphoid tissue. Clin Exp Immunol. 1982 May;48(2):348–352. [PMC free article] [PubMed] [Google Scholar]
  6. Heinen E., Lilet-Leclercq C., Mason D. Y., Stein H., Boniver J., Radoux D., Kinet-Denoël C., Simar L. J. Isolation of follicular dendritic cells from human tonsils and adenoids. II. Immunocytochemical characterization. Eur J Immunol. 1984 Mar;14(3):267–273. doi: 10.1002/eji.1830140312. [DOI] [PubMed] [Google Scholar]
  7. Heinen E., Radoux D., Kinet-Denoël C., Simar L. J. Colloidal gold, a useful marker for antigen localization on follicular dendritic cells. J Immunol Methods. 1983 May 13;59(3):361–368. doi: 10.1016/0022-1759(83)90196-5. [DOI] [PubMed] [Google Scholar]
  8. Herd Z. L., Ada G. L. The retention of 125I-immunoglobulins, IgG subunits and antigen-antibody complexes in rat footpads and draining lymph nodes. Aust J Exp Biol Med Sci. 1969 Feb;47(1):63–72. doi: 10.1038/icb.1969.5. [DOI] [PubMed] [Google Scholar]
  9. Klaus G. G., Humphrey J. H., Kunkl A., Dongworth D. W. The follicular dendritic cell: its role in antigen presentation in the generation of immunological memory. Immunol Rev. 1980;53:3–28. doi: 10.1111/j.1600-065x.1980.tb01038.x. [DOI] [PubMed] [Google Scholar]
  10. Kunkl A., Klaus G. G. The generation of memory cells. IV. Immunization with antigen-antibody complexes accelerates the development of B-memory cells, the formation of germinal centres and the maturation of antibody affinity in the secondary response. Immunology. 1981 Jun;43(2):371–378. [PMC free article] [PubMed] [Google Scholar]
  11. Kölsch E., Oberbarnscheidt J., Brüner K., Heuer J. The Fc-receptor: its role in the transmission of differentiation signals. Immunol Rev. 1980;49:61–78. doi: 10.1111/j.1600-065x.1980.tb00426.x. [DOI] [PubMed] [Google Scholar]
  12. Lilet-Leclercq C., Radoux D., Heinen E., Kinet-Denoël C., Defraigne J. O., Houben-Defresne M. P., Simar L. J. Isolation of follicular dendritic cells from human tonsils and adenoids. I. Procedure and morphological characterization. J Immunol Methods. 1984 Feb 10;66(2):235–244. doi: 10.1016/0022-1759(84)90334-x. [DOI] [PubMed] [Google Scholar]
  13. Michl J., Unkeless J. C., Pieczonka M. M., Silverstein S. C. Modulation of Fc receptors of mononuclear phagocytes by immobilized antigen-antibody complexes. Quantitative analysis of the relationship between ligand number and Fc receptor response. J Exp Med. 1983 Jun 1;157(6):1746–1757. doi: 10.1084/jem.157.6.1746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morgan E. L., Weigle W. O. Potentiation of specific human in vitro immune responses by the Fc portion of human immunoglobulin. Clin Exp Immunol. 1983 Aug;53(2):505–511. [PMC free article] [PubMed] [Google Scholar]
  15. Nossal G. J., Ada G. L., Austin C. M., Pye J. Antigens in immunity. 8. Localization of 125-I-labelled antigens in the secondary response. Immunology. 1965 Oct;9(4):349–357. [PMC free article] [PubMed] [Google Scholar]
  16. Papamichail M., Gutierrez C., Embling P., Johnson P., Holborow E. J., Pepys M. B. Complement dependence of localisation of aggregated IgG in germinal centres. Scand J Immunol. 1975;4(4):343–347. doi: 10.1111/j.1365-3083.1975.tb02635.x. [DOI] [PubMed] [Google Scholar]
  17. Radoux D., Heinen E., Kinet-Denoël C., Tihange E., Simar L. Precise localization of antigens on follicular dendritic cells. Cell Tissue Res. 1984;235(2):267–274. doi: 10.1007/BF00217850. [DOI] [PubMed] [Google Scholar]
  18. Revillard J. P., Lê Thi Bich-Thuy L. T. Régulation de la production d'immunoglobulines: rôle des récepteurs cellulaires de la partie Fc de ces molécules. Ann Immunol (Paris) 1982 Sep-Oct;133D(2):199–210. [PubMed] [Google Scholar]
  19. Romball C. G., Ulevitch R. J., Weigle W. O. Role of C3 in the regulation of a splenic PFC response in rabbits. J Immunol. 1980 Jan;124(1):151–155. [PubMed] [Google Scholar]
  20. Suzuki T., Sadasivan R., Saito-Taki T., Stechschulte D. J., Balentine L., Helmkamp G. M., Jr Studies of Fc gamma receptors of human B lymphocytes: phospholipase A2 activity of Fc gamma receptors. Biochemistry. 1980 Dec 23;19(26):6037–6044. doi: 10.1021/bi00567a014. [DOI] [PubMed] [Google Scholar]
  21. Tew J. G., Phipps R. P., Mandel T. E. The maintenance and regulation of the humoral immune response: persisting antigen and the role of follicular antigen-binding dendritic cells as accessory cells. Immunol Rev. 1980;53:175–201. doi: 10.1111/j.1600-065x.1980.tb01044.x. [DOI] [PubMed] [Google Scholar]

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