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. 1985 Jun;55(2):317–324.

In vitro generated mast cells express natural cytotoxicity against tumour cells.

P Ghiara, D Boraschi, L Villa, G Scapigliati, C Taddei, A Tagliabue
PMCID: PMC1453603  PMID: 3924820

Abstract

Bone marrow cells from mice were cultured in vitro in the presence of interleukin-3 (IL-3). After 2 weeks, the majority of the cells differentiated towards mast cells as judged by morphological and biochemical criteria. When populations of 99% IL-3-dependent mast cells (IL-3 MC) were tested for their anti-tumour activity in vitro, it was found that they can express natural cytotoxicity (NC) but not other natural reactivities. Moreover, the effector cells were not positive for Thy 1, Lyt 1 and Lyt 2 markers. The capability of mast cells to express NC activity seems to be related to their in vitro differentiation, since mast cell deficient W/Wv mice had normal NC activity. Thus, IL-3 MC must be added to the variety of cells capable of expressing natural anti-tumour reactivities in vitro.

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Selected References

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