Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1985 Sep;56(1):43–50.

Immunological responses to monoassociated Bifidobacterium longum and their relation to prevention of bacterial invasion.

S Yamazaki, K Machii, S Tsuyuki, H Momose, T Kawashima, K Ueda
PMCID: PMC1453656  PMID: 4043995

Abstract

After monoassociation of Bifidobacterium longum with germ-free BALB/c mice (nu/+, heterozygous to nu gene), B. longum was recovered (10(2)-10(4) viable units per organ) from the mesenteric lymph nodes, liver and kidneys for 2 weeks post intragastric inoculation, but not after 4 weeks and later. Cessation of bacterial translocation was not observed in monoassociated nude (nu/nu) mice. Anti-B. longum IgA antibody was detected by ELISA using B. longum cell wall antigen in bile from Week 1 and in ileac wall extract from Week 8 post-association in both nu/+ and nu/nu mice. Total IgA levels in bile, ileac wall extract and caecal contents were also elevated in both mice after monoassociation. Cell-mediated immunity measured by the footpad test and macrophage migration inhibition test using B. longum protein fraction was detected in nu/+ mice in Week 4 and later, but not in nu/nu mice. Nu/nu mice reconstituted 4 weeks prior to monoassociation with lymphocytes from flora bearing nu/+ mice developed delayed footpad reactivity and bacterial translocation stopped after 4 weeks. Cell-mediated immunity rather than IgA antibody correlated well with the cessation of translocation.

Full text

PDF
46

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe C., Homma Y., Ueda K., Saegusa J., Nakayama M. Studies on pathogenicity and persistence of Propionibacterium acnes in mice. Zentralbl Bakteriol Mikrobiol Hyg A. 1981 Dec;251(2):223–229. [PubMed] [Google Scholar]
  2. Berg R. D., Garlington A. W. Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. Infect Immun. 1979 Feb;23(2):403–411. doi: 10.1128/iai.23.2.403-411.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg R. D., Owens W. E. Inhibition of translocation of viable Escherichia coli from the gastrointestinal tract of mice by bacterial antagonism. Infect Immun. 1979 Sep;25(3):820–827. doi: 10.1128/iai.25.3.820-827.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bienenstock J., Befus A. D. Mucosal immunology. Immunology. 1980 Oct;41(2):249–270. [PMC free article] [PubMed] [Google Scholar]
  5. Carter P. B., Collins F. M. The route of enteric infection in normal mice. J Exp Med. 1974 May 1;139(5):1189–1203. doi: 10.1084/jem.139.5.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins F. M., Carter P. B. Cellular immunity in enteric disease. Am J Clin Nutr. 1974 Dec;27(12):1424–1433. doi: 10.1093/ajcn/27.12.1424. [DOI] [PubMed] [Google Scholar]
  7. Collins F. M., Mackaness G. B. Delayed hypersensitivity and arthus reactivity in relation to host resistance in salmonella-infected mice. J Immunol. 1968 Nov;101(5):830–845. [PubMed] [Google Scholar]
  8. Collins F. M. Mucosal defenses against Salmonella infection in the mouse. J Infect Dis. 1979 May;139(5):503–510. doi: 10.1093/infdis/139.5.503. [DOI] [PubMed] [Google Scholar]
  9. Fimmel P. J., Keast D. Studies on in vitro leucocyte migration inhibition. Aust J Exp Biol Med Sci. 1974 Oct;52(5):745–753. doi: 10.1038/icb.1974.74. [DOI] [PubMed] [Google Scholar]
  10. Frederick G. T., Bohl E. H. Local and systemic cell-mediated immunity against transmissible gastroenteritis, an intestinal viral infection of swine. J Immunol. 1976 Apr;116(4):1000–1004. [PubMed] [Google Scholar]
  11. Huntley J., Newby T. J., Bourne F. J. The cell-mediated immune response of the pig to orally administered antigen. Immunology. 1979 May;37(1):225–230. [PMC free article] [PubMed] [Google Scholar]
  12. Iwai H., Yamaguchi R., Otsuka Y., Ueda K., Saito M. Immunoglobulin classes of anti-Sendai virus antibody detected by ELISA in infected nude mouse serum. Microbiol Immunol. 1984;28(4):481–491. doi: 10.1111/j.1348-0421.1984.tb00699.x. [DOI] [PubMed] [Google Scholar]
  13. LeFevre M. E., Hammer R., Joel D. D. Macrophages of the mammalian small intestine: a review. J Reticuloendothel Soc. 1979 Nov;26(5):553–573. [PubMed] [Google Scholar]
  14. MacDonald T. T., Carter P. B. Cell-mediated immunity to intestinal infection. Infect Immun. 1980 May;28(2):516–523. doi: 10.1128/iai.28.2.516-523.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maejima K., Tajima Y. Association of gnotobiotic mice with various organisms isolated from conventional mice. Jpn J Exp Med. 1973 Aug;43(4):289–296. [PubMed] [Google Scholar]
  16. Nash D. R., Crabbé P. A., Bazin H., Eyssen H., Heremans J. F. Specific and non-specific immunoglobulin synthesis in germfree mice immunized with ferritin by different routes. Experientia. 1969 Oct 15;25(10):1094–1096. doi: 10.1007/BF01901452. [DOI] [PubMed] [Google Scholar]
  17. Owens W. E., Berg R. D. Bacterial translocation from the gastrointestinal tract of athymic (nu/nu) mice. Infect Immun. 1980 Feb;27(2):461–467. doi: 10.1128/iai.27.2.461-467.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Perrotto J. L., Hang L. M., Isselbacher K. J., Warren K. S. Systemic cellular hypersensitivity induced by an intestinally absorbed antigen. J Exp Med. 1974 Jul 1;140(1):296–299. doi: 10.1084/jem.140.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Spalding D. M., Williamson S. I., Koopman W. J., McGhee J. R. Preferential induction of polyclonal IgA secretion by murine Peyer's patch dendritic cell-T cell mixtures. J Exp Med. 1984 Sep 1;160(3):941–946. doi: 10.1084/jem.160.3.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ueda K., Yamazaki S., Someya S. Studies on tubercle bacillus infection in germ-free mice. J Reticuloendothel Soc. 1972 Nov;12(5):545–563. [PubMed] [Google Scholar]
  21. Ueda K., Yamazaki S., Yamamoto S., Someya S. Spleen cell transfer induces T cell-dependent granulomas in tuberculous nude mice. J Reticuloendothel Soc. 1982 Jun;31(6):469–478. [PubMed] [Google Scholar]
  22. Wolochow H., Hildebrand G. J., Lamanna C. Translocation of microorganisms across the intestinal wall of the rat: effect of microbial size and concentration. J Infect Dis. 1966 Oct;116(4):523–528. doi: 10.1093/infdis/116.4.523. [DOI] [PubMed] [Google Scholar]
  23. van der Waaij D., Berghuis-de Vries J. M., Lekkerkerk-van der Wees Colonization resistance of the digestive tract and the spread of bacteria to the lymphatic organs in mice. J Hyg (Lond) 1972 Jun;70(2):335–342. doi: 10.1017/s0022172400022385. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES