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. 1985 Oct;56(2):359–366.

Glucocorticoids inhibit IgE receptor expression on the human monocyte cell line U937.

A Náray-Fejes-Tóth, G G Cornwell, P M Guyre
PMCID: PMC1453685  PMID: 2932386

Abstract

Cells of a human monocyte-like cell line (U937) were analysed for IgE Fc receptors (Fc epsilon R) before and after glucocorticoid treatment. Specific binding of human myeloma IgE (Sha) was measured by 125I-labelled IgE, and by fluorescein-labelled IgE monitored by flow cytometry. Treatment of cells with dexamethasone or other steroids with glucocorticoid activity caused a significant decrease in Fc epsilon R expression. The inhibition was dose dependent, with a half-maximal effect at 20 nM dexamethasone, a concentration which is near to the dissociation constant of glucocorticoid receptors for dexamethasone. Inhibition of Fc epsilon R was significant beginning 8 h following glucocorticoid treatment and reached a plateau at 24 hr. The Ka for IgE binding was similar for control and dexamethasone-treated cells, while the number of IgE binding sites was decreased by 50-60%. Culture supernatants from dexamethasone-treated U937 cells which were concentrated 10-fold and depleted of free steroid did not affect Fc epsilon R expression. These results demonstrate that glucocorticoids can directly decrease the number of Fc epsilon R. This effect could participate in the glucocorticoid-induced suppression of IgE-mediated allergic reactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. L., Spiegelberg H. L. Macrophage receptors for IgE: binding of IgE to specific IgE Fc receptors on a human macrophage cell line, U937. J Immunol. 1981 Jun;126(6):2470–2473. [PubMed] [Google Scholar]
  2. Boltz-Nitulescu G., Plummer J. M., Spiegelberg H. L. Fc receptors for IgE on mouse macrophages and macrophage-like cell lines. J Immunol. 1982 May;128(5):2265–2268. [PubMed] [Google Scholar]
  3. Capron A., Dessaint J. P., Joseph M., Rousseaux R., Capron M., Bazin H. Interaction between IgE complexes and macrophages in the rat: a new mechanism of macrophage activation. Eur J Immunol. 1977 May;7(5):315–322. doi: 10.1002/eji.1830070515. [DOI] [PubMed] [Google Scholar]
  4. Crabtree G. R., Munck A., Smith K. A. Glucocorticoids inhibit expression of Fc receptors on the human granulocytic cell line HL-60. Nature. 1979 May 24;279(5711):338–339. doi: 10.1038/279338a0. [DOI] [PubMed] [Google Scholar]
  5. Gonzalez-Molina A., Spiegelberg H. L. A subpopulation of normal human peripheral B lymphcytes that bind IgE. J Clin Invest. 1977 Apr;59(4):616–624. doi: 10.1172/JCI108679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guyre P. M., Morganelli P. M., Miller R. Recombinant immune interferon increases immunoglobulin G Fc receptors on cultured human mononuclear phagocytes. J Clin Invest. 1983 Jul;72(1):393–397. doi: 10.1172/JCI110980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hattori T., Hirata F., Hoffman T., Hizuta A., Herberman R. B. Inhibition of human natural killer (NK) activity and antibody dependent cellular cytotoxicity (ADCC) by lipomodulin, a phospholipase inhibitory protein. J Immunol. 1983 Aug;131(2):662–665. [PubMed] [Google Scholar]
  8. Hattori T., Hoffman T., Hirata F. Differentiation of a histiocytic lymphoma cell line by lipomodulin, a phospholipase inhibitory protein. Biochem Biophys Res Commun. 1983 Mar 16;111(2):551–559. doi: 10.1016/0006-291x(83)90342-x. [DOI] [PubMed] [Google Scholar]
  9. Hirata F., Iwata M. Role of lipomodulin, a phospholipase inhibitory protein, in immunoregulation by thymocytes. J Immunol. 1983 Apr;130(4):1930–1936. [PubMed] [Google Scholar]
  10. Melewicz F. M., Plummer J. M., Spiegelberg H. L. Comparison of the Fc receptors for IgE on human lymphocytes and monocytes. J Immunol. 1982 Aug;129(2):563–569. [PubMed] [Google Scholar]
  11. Melewicz F. M., Spiegelberg H. L. Fc receptors for IgE on a subpopulation of human peripheral blood monocytes. J Immunol. 1980 Sep;125(3):1026–1031. [PubMed] [Google Scholar]
  12. Melewicz F. M., Zeiger R. S., Mellon M. H., O'Connor R. D., Spiegelberg H. L. Increased IgE-dependent cytotoxicity by blood mononuclear cells of allergic patients. Clin Exp Immunol. 1981 Mar;43(3):526–533. [PMC free article] [PubMed] [Google Scholar]
  13. Munson P. J., Rodbard D. Ligand: a versatile computerized approach for characterization of ligand-binding systems. Anal Biochem. 1980 Sep 1;107(1):220–239. doi: 10.1016/0003-2697(80)90515-1. [DOI] [PubMed] [Google Scholar]
  14. Náray-Fejes-Tóth A., Guyre P. M. Recombinant human immune interferon induces increased IgE receptor expression on the human monocyte cell line U-937. J Immunol. 1984 Oct;133(4):1914–1919. [PubMed] [Google Scholar]
  15. Paavonen T., Andersson L. C., Kontula K. Lack of correlation between the glucocorticoid receptor density and the in vitro growth-inhibitory effect of dexamethasone in human leukemia cell lines. J Recept Res. 1980;1(3):459–472. doi: 10.3109/10799898009038793. [DOI] [PubMed] [Google Scholar]
  16. Rouzer C. A., Scott W. A., Hamill A. L., Liu F. T., Katz D. H., Cohn Z. A. Secretion of leukotriene C and other arachidonic acid metabolites by macrophages challenged with immunoglobulin E immune complexes. J Exp Med. 1982 Oct 1;156(4):1077–1086. doi: 10.1084/jem.156.4.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schleimer R. P., Lichtenstein L. M., Gillespie E. Inhibition of basophil histamine release by anti-inflammatory steroids. Nature. 1981 Jul 30;292(5822):454–455. doi: 10.1038/292454a0. [DOI] [PubMed] [Google Scholar]
  18. Schreiber A. D., Parsons J., McDermott P., Cooper R. A. Effect of corticosteroids on the human monocyte IgG and complement receptors. J Clin Invest. 1975 Nov;56(5):1189–1197. doi: 10.1172/JCI108196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Spiegelberg H. L., O'Connor R. D., Simon R. A., Mathison D. A. Lymphocytes with immunoglobulin E Fc receptors in patients with atopic disorders. J Clin Invest. 1979 Sep;64(3):714–720. doi: 10.1172/JCI109514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Titus J. A., Haynes B. F., Thomas C. A., Fauci A. S., Segal D. M. Analysis of Fc gamma receptors on human peripheral blood leukocytes by flow microfluorometry. I. Receptor distributions on monocytes, T gamma cells and cells labeled with the 3Al anti-T cell monoclonal antibody. Eur J Immunol. 1982 Jun;12(6):474–479. doi: 10.1002/eji.1830120605. [DOI] [PubMed] [Google Scholar]
  21. Vallee E., Gougat J., Navarro J., Delahayes J. F. Anti-inflammatory and platelet anti-aggregant activity of phospholipase-A2 inhibitors. J Pharm Pharmacol. 1979 Sep;31(9):588–592. doi: 10.1111/j.2042-7158.1979.tb13597.x. [DOI] [PubMed] [Google Scholar]
  22. Yodoi J., Hirashima M., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. VI. Suppressive effect of glucocorticoids on the expression of Fc epsilon receptors and glycosylation of IgE-binding factors. J Immunol. 1981 Aug;127(2):471–476. [PubMed] [Google Scholar]
  23. Yodoi J., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fc epsilon receptors. J Immunol. 1979 Jun;122(6):2577–2583. [PubMed] [Google Scholar]

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