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. 1985 Nov;56(3):393–399.

Shared idiotypes are expressed on mouse and human anti-DNA autoantibodies.

A Morgan, D A Isenberg, Y Naparstek, J Rauch, D Duggan, R Khiroya, N A Staines, A Schattner
PMCID: PMC1453751  PMID: 3878322

Abstract

The expression of common idiotypes on human and mouse anti-DNA monoclonal autoantibodies made by hybridomas was examined by their competitive binding to anti-idiotype antibodies. Some murine autoantibodies inhibited the binding of a human anti-DNA autoantibody 16/6 to monoclonal or polyclonal anti-idiotypic antibodies. Another human antibody (134) was not inhibited in its binding to homologous anti-idiotypic antibodies. The expression of the human 16/6 idiotype on mouse antibodies was restricted to those that had a specificity similar to the 16/6 antibody itself, their major properties being that they reacted more strongly with single stranded DNA (ssDNA) than double stranded DNA (dsDNA). One mouse antibody expressing the 16/6 idiotype also bound weakly to RNA. The results imply structural similarities between the binding sites of the antibodies in the two species, and are consistent with evolutionary conservation of V genes coding for primitive ancestral antibodies that react with DNA and become diversified through somatic mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews B. S., Eisenberg R. A., Theofilopoulos A. N., Izui S., Wilson C. B., McConahey P. J., Murphy E. D., Roths J. B., Dixon F. J. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978 Nov 1;148(5):1198–1215. doi: 10.1084/jem.148.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andrzejewski C., Jr, Stollar B. D., Lalor T. M., Schwartz R. S. Hybridoma autoantibodies to DNA. J Immunol. 1980 Mar;124(3):1499–1502. [PubMed] [Google Scholar]
  3. André-Schwartz J., Datta S. K., Shoenfeld Y., Isenberg D. A., Stollar B. D., Schwartz R. S. Binding of cytoskeletal proteins by monoclonal anti-DNA lupus autoantibodies. Clin Immunol Immunopathol. 1984 May;31(2):261–271. doi: 10.1016/0090-1229(84)90246-0. [DOI] [PubMed] [Google Scholar]
  4. Diamond B., Scharff M. D. Somatic mutation of the T15 heavy chain gives rise to an antibody with autoantibody specificity. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5841–5844. doi: 10.1073/pnas.81.18.5841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eilat D., Fischel R., Zlotnick A. A central anti-DNA idiotype in human and murine systemic lupus erythematosus. Eur J Immunol. 1985 Apr;15(4):368–375. doi: 10.1002/eji.1830150412. [DOI] [PubMed] [Google Scholar]
  6. Faaber P., Capel P. J., Rijke G. P., Vierwinden G., van de Putte L. B., Koene R. A. Cross-reactivity of anti-DNA antibodies with proteoglycans. Clin Exp Immunol. 1984 Mar;55(3):502–508. [PMC free article] [PubMed] [Google Scholar]
  7. Griffiths G. M., Berek C., Kaartinen M., Milstein C. Somatic mutation and the maturation of immune response to 2-phenyl oxazolone. Nature. 1984 Nov 15;312(5991):271–275. doi: 10.1038/312271a0. [DOI] [PubMed] [Google Scholar]
  8. Hahn B. H., Ebling F. M. A public idiotypic determinant is present on spontaneous cationic IgG antibodies to DNA from mice of unrelated lupus-prone strains. J Immunol. 1984 Dec;133(6):3015–3019. [PubMed] [Google Scholar]
  9. Hahn B. H., Ebling F., Freeman S., Clevinger B., Davie J. Production of monoclonal murine antibodies to DNA by somatic cell hybrids. Arthritis Rheum. 1980 Aug;23(8):942–945. doi: 10.1002/art.1780230811. [DOI] [PubMed] [Google Scholar]
  10. Koike T., Nagasawa R., Nagata N., Shirai T. Specificity of mouse hybridoma antibodies to DNA. Immunol Lett. 1982 Feb;4(2):93–97. doi: 10.1016/0165-2478(82)90006-2. [DOI] [PubMed] [Google Scholar]
  11. Lafer E. M., Rauch J., Andrzejewski C., Jr, Mudd D., Furie B., Furie B., Schwartz R. S., Stollar B. D. Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med. 1981 Apr 1;153(4):897–909. doi: 10.1084/jem.153.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lake R. A., Morgan A., Henderson B., Staines N. A. A key role for fibronectin in the sequential binding of native dsDNA and monoclonal anti-DNA antibodies to components of the extracellular matrix: its possible significance in glomerulonephritis. Immunology. 1985 Feb;54(2):389–395. [PMC free article] [PubMed] [Google Scholar]
  13. Marion T. N., Lawton A. R., 3rd, Kearney J. F., Briles D. E. Anti-DNA autoantibodies in (NZB X NZW)F1 mice are clonally heterogeneous, but the majority share a common idiotype. J Immunol. 1982 Feb;128(2):668–674. [PubMed] [Google Scholar]
  14. Naparstek Y., Duggan D., Schattner A., Madaio M. P., Goni F., Frangione B., Stollar B. D., Kabat E. A., Schwartz R. S. Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies. J Exp Med. 1985 Jun 1;161(6):1525–1538. doi: 10.1084/jem.161.6.1525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rauch J., Massicotte H., Tannenbaum H. Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics. J Immunol. 1985 Jan;134(1):180–186. [PubMed] [Google Scholar]
  16. Rauch J., Tannenbaum H., Stollar B. D., Schwartz R. S. Monoclonal anti-cardiolipin antibodies bind to DNA. Eur J Immunol. 1984 Jun;14(6):529–534. doi: 10.1002/eji.1830140609. [DOI] [PubMed] [Google Scholar]
  17. Rubin R. L., Balderas R. S., Tan E. M., Dixon F. J., Theofilopoulos A. N. Multiple autoantigen binding capabilities of mouse monoclonal antibodies selected for rheumatoid factor activity. J Exp Med. 1984 May 1;159(5):1429–1440. doi: 10.1084/jem.159.5.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shoenfeld Y., Isenberg D. A., Rauch J., Madaio M. P., Stollar B. D., Schwartz R. S. Idiotypic cross-reactions of monoclonal human lupus autoantibodies. J Exp Med. 1983 Sep 1;158(3):718–730. doi: 10.1084/jem.158.3.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shoenfeld Y., Rauch J., Massicotte H., Datta S. K., André-Schwartz J., Stollar B. D., Schwartz R. S. Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. N Engl J Med. 1983 Feb 24;308(8):414–420. doi: 10.1056/NEJM198302243080802. [DOI] [PubMed] [Google Scholar]
  20. Solomon G., Schiffenbauer J., Keiser H. D., Diamond B. Use of monoclonal antibodies to identify shared idiotypes on human antibodies to native DNA from patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1983 Feb;80(3):850–854. doi: 10.1073/pnas.80.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tan E. M. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982;33:167–240. doi: 10.1016/s0065-2776(08)60836-6. [DOI] [PubMed] [Google Scholar]
  22. Tron F., Jacob L., Bach J. F. Murine monoclonal anti-DNA antibodies with an absolute specificity for DNA have a large amount of idiotypic diversity. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6024–6027. doi: 10.1073/pnas.80.19.6024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tron F., Le Guern C., Cazenave P. A., Bach J. F. Intrastrain recurrent idiotypes among anti-DNA antibodies of (NZB x NZW)F1 hybrid mice. Eur J Immunol. 1982 Sep;12(9):761–766. doi: 10.1002/eji.1830120911. [DOI] [PubMed] [Google Scholar]

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